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VMRD Products Catalog
Stilts Adams
About About
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Veterinary Diagnostic
Test Kits & Reagents
Veterinary Diagnostic
Test Kits & Reagents
On behalf of the whole VMRD team, thank you for your business!
VMRD was founded with the goal of bringing better tests to you, our
customers. Better tests yield better diagnoses, better relationships,
better outcomes, better bottom lines, and better days.
Our products and services team members strive every day to
accomplish the better test goal. If we ever fail to achieve this goal,
please do not hesitate to contact any member of the VMRD team,
including me personally, so that we can make it right. That is the
way we would want to be treated, and we will do our best to treat
you that way.
May you have only good days at the bench!
Soli Deo gloria,
Ethan Adams, CEO
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CONTENTS
Diagnostic Test Kits ......................................................................................................................... 1-10
Point-of-Care.....................................................................................................................................11-13
Molecular .......................................................................................................................................... 14-16
Immunology Reagents ......................................................................................................................17
FA Reagents ................................................................................................................................... 18-26
Antibodies ....................................................................................................................................... 27-28
Equipment .......................................................................................................................................29-30
Biologics Testing Services .............................................................................................................. 31
Ordering Information ........................................................................................................................32
Sensitivity & Specificity in Perspective
Relative sensitivity and specificity values are
calculated from data generated by diagnostic
laboratory field testing (available upon request).
These values are provided as guidelines only and
should not be construed as the absolute sensitivity
and specificity of the test in question for any
population subset.
Component 283-2
A. Antigen-Coated Plates 2 plate
B. Positive Control 3.6 ml
C. Negative Control 3.6 ml
D. 100X Antibody-Peroxidase Conjugate 0.3 ml
E. Conjugate Diluting Buffer 30 ml
F. 10X Wash Solution Concentrate 120 ml
G. Substrate Solution 30 ml
H. Stop Solution 30 ml
Test Kit Insert
KIT CONTENTS
OVERVIEW OF KIT PROCEDURE
1. Transfer 50 µl of samples and controls
into wells of Antigen-Coated Plate
2. Incubate 60 minutes at room temperature
3. Wash 2 times with Wash Solution
4. Add 50 µl of Antibody-Peroxidase Conjugate
5. Incubate 20 minutes at room temperature
6. Wash 4 times with Wash Solution
7. Add 50 µl of Substrate Solution
8. Incubate 20 minutes at room temperature
9. Add 50 µl of Stop Solution
10. Read at 620-650 nm
Setting the Standard
in the Diagnosis of Anaplasmosis
VMRD’s
Anaplasma
Antibody Test Kit is a
competitive, enzyme-linked, immunosorbent
assay (cELISA) for the detection of antibodies
specific for
Anaplasma
in bovine serum
samples. It is intended to provide results
that will give guidance about the presence
of
Anaplasma
infection in bovine species.
Sensitivity and specificity are more than four-
fold better than the complement fixation test
(CFT) which was the former gold standard test.
This OIE-recommended cELISA is a
breakthrough in diagnosis of anaplasmosis
in persistently-infected animals. It detects
antibodies to
Anaplasma
marginale,
Anaplasma ovis,
and
Anaplasma centrale.
Notwithstanding some recent publications, we
do not believe that the assay should be relied
upon for detection of antibodies to
Anaplasma
phagocytophilum.
The kit is available in
2-plate format with breakaway stripwells.
About Anaplasmosis
Anaplasmosis is a non-contagious, arthropod-
borne, parasitic disease of ruminants that
results in significant economic losses to the
cattle industry. The disease in cattle is caused
by
Anaplasma marginale,
recently classified
in geno-group II of
Ehrlichiae. Anaplasma
marginale
is an intra-erythrocytic parasite that
causes severe anemia, abortion, weight loss,
jaundice and death. Diagnosis of the acute
disease is based upon clinical signs, anemia
and finding of
Anaplasma
inclusion bodies
in erythrocytes. Animals surviving the acute
phase become lifelong carriers. Ticks transmit
the infection from carriers to naïve cattle, which
develop clinical disease. Cycles of rickettsemia
in carriers fluctuate between 10
2.5
and 10
7
infected erythrocytes per ml, levels generally
undetectable by Giemsa staining. Carriers can
be identified by detection of serum antibodies
to
A. marginale
with VMRD’s
Anaplasma
Antibody Test Kit.
Formula for calculating % inhibition:
%I = 100 [1-(Sample OD ÷ Negative Control OD)]
Samples producing <30% inhibition are negative. Samples
producing 30% inhibition are positive.
For the test to be valid, the mean OD of the Negative
Control must range from 0.40 to 2.10. The percent
inhibition of the Positive Control must be 30%.
DIAGNOSTIC TEST KITS | BOVINE
Veterinary Medical Research & Development
CATALOG NO.
283-2
SPECIES SAMPLE
Bovine
SAMPLE
Serum
SENSITIVITY
100%
SPECIFICITY
99.7%
ASSAY TIME
100 minutes
CONFIGURATION
2 stripwell plates
TESTS
182
CATALOG NO.
283-WASH
120mL of
lot-specific 10X
Wash Solution
Concentrate
See Sensitivity & Specificity
in Perspective on TOC page
ANAPLASMOSIS
Anaplasma
Antibody
Test Kit
cELISA v2
1
VMRD’s highly-sensitive and specific enzyme-
linked, immunosorbent assay (ELISA) kit
detects antibodies to bovine leukemia virus
(BLV) glycoprotein 51 (gp51) in bovine sera.
Sample serum antibodies bind to BLV gp51
molecules attached to the plastic wells of
the microtiter plate. Binding of these serum
antibodies is detected by reaction with
horseradish peroxidase (HRP)-affinity-purified
goat antibodies to bovine immunoglobulins.
Attached HRP-antibodies are detected
by addition of enzyme substrate and
quantitated by subsequent blue color product
development. Strong color development
indicates the presence of antibodies to BLV
gp51 in the sample serum. Very weak or no
color development indicates the absence
of detectable antibodies to BLV gp51 in the
sample serum. VMRD’s Bovine Leukemia Virus
Antibody Test Kit is USDA-approved for export
testing and is available in breakaway stripwell
format. The assay requires that an ELISA plate
reader be used for accurate results.
About Bovine Leukosis
Enzootic Bovine Leukosis (EBL) is an infectious,
non-contagious viral disease of cattle. It is
caused by BLV, an oncogenic delta retrovirus,
which results in proliferation of B lymphocytes.
Infection with BLV may lead to persistent
lymphocytosis and in some adult cattle to the
development of tumors with associated signs.
The spread of disease from the introduction
into a herd may reach enzootic proportions.
Transmission of BLV occurs between animals
primarily by transfer of B lymphocytes in
blood. Trauma, use of common bleeding
needles, and surgical procedures are the main
means of transmission. It is rarely vertically
transmitted. Most BLV infections are inapparent,
with approximately 5% of animals developing
clinical signs. AGID and ELISA tests are used to
identify carrier cattle. Control programs for EBL
include testing and removal of positive animals.
Several European countries which have
instituted eradication programs also require
that imported cattle be free of BLV.
Component 283-2
A. Antigen-Coated Plates 2 plate
B. Positive Control 3.6 ml
C. Negative Control 3.6 ml
D. 100X Antibody-Peroxidase Conjugate 0.3 ml
E. Conjugate Diluting Buffer 30 ml
F. 10X Wash Solution Concentrate 120 ml
G. Substrate Solution 30 ml
H. Stop Solution 30 ml
Test Kit Insert
DIAGNOSTIC TEST KITS | BOVINE
800.222.8673 | www.vmrd.com
CATALOG NO.
284-2
SPECIES SAMPLE
Bovine
SAMPLE
Serum
SENSITIVITY
98%
SPECIFICITY
100%
ASSAY TIME
60 minutes
CONFIGURATION
2 stripwell plates
TESTS
182
CATALOG NO.
284-WASH
120mL of
lot-specific 10X
Wash Solution
Concentrate
See Sensitivity & Specificity
in Perspective on TOC page
BOVINE LEUKOSIS
Bovine Leukemia Virus
Antibody Test Kit
ELISA
Component 284-2
A. Antigen-Coated Plates 2 plate
B. Positive Control 4 ml
C. Negative Control 4 ml
D. 100X Antibody-Peroxidase Conjugate 0.3 ml
E. Conjugate Diluting Buffer 30 ml
F. 10X Wash Solution Concentrate 120 ml
G. Serum Diluting Buffer 60 ml
H. Substrate Solution 30 ml
I. Stop Solution 30 ml
Test Kit Insert
KIT CONTENTS
OVERVIEW OF KIT PROCEDURE
1. Dilute serum samples 1/25 with Serum Diluting
Buffer
2. Transfer 50 µl of each sample and controls into
wells of the Antigen-Coated Plate
3. Incubate 20 minutes at room temperature
4. Wash 3 times with Wash Solution
5. Add 50 µl of Antibody-Peroxidase Conjugate
6. Incubate 20 minutes at room temperature
7. Wash 3 times with Wash Solution
8. Add 50 µl of Substrate Solution
9. Incubate 20 minutes at room temperature
10. Add 50 µl of Stop Solution
11. Read at 620-650 nm
All samples with mean OD values greater than or equal
to the mean OD of the Positive Controls are considered
positive for BLV. All samples with mean OD values less
than the mean of the Positive Controls are negative for
BLV.
For the test to be valid, the mean OD of the Negative
Controls must be less than 0.200. The mean OD of the
Positive Controls must be 0.250 and <2.000.
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DIAGNOSTIC TEST KITS | BOVINE
Veterinary Medical Research & Development
CATALOG NO.
280-2
SPECIES SAMPLE
Bovine
SAMPLE
Serum
SENSITIVITY
96%
SPECIFICITY
99%
ASSAY TIME
100 minutes
CONFIGURATION
2 stripwell plates
TESTS
184
CATALOG NO.
280-WASH
120mL of
lot-specific 10X
Wash Solution
Concentrate
See Sensitivity & Specificity
in Perspective on TOC page
VMRD’s
Neospora
test is a competitive,
enzyme-linked immunosorbent assay (cELISA)
that detects antibodies against
Neospora
caninum
in cattle sera. Our competitive ELISA
format allows other species to be tested,
but validation has been completed only on
cattle. An immunodominant surface protein
of 65 kDa is captured on the antigen plate
using a monoclonal antibody. Another HRP-
conjugated monoclonal antibody competes
with serum antibodies for a specific epitope
on p65. Sensitivity and specificity studies
confirm the high accuracy of this kit. In a mass
screening of 4,323 sera of unknown serologic
status, only 5% of sera fell within ±5% of the
cut-off value, demonstrating a clear distinction
between positive and negative sera (bimodal
distribution).
About Neosporosis
Neosporosis has been identified across the
world in various species, including dogs,
cattle, sheep, goats, and horses. It is caused
by
Neospora
caninum,
a protozoan parasite
closely related to
Toxoplasma gondii.
Although
canids have been identified as the definitive
host for
N. caninum,
it is not known if there
are other definitive hosts. No clinical signs are
noted in cows that abort due to
N. caninum
either prior to the abortion or post-abortion.
Aborted fetuses are usually autolyzed with no
gross lesions and placentas are not retained.
Abortions have been diagnosed in both heifers
and cows from 3 months gestation to term. A
majority (78%) of
N. caninum
abortions occur
between 4 and 6 months gestation. This
pattern of mid-gestation abortion is distinct
from other diagnosed causes of infectious
abortion in dairy cattle which tend to occur
later in gestation. In dogs,
N. caninum
infection
causes neuromuscular paralysis. Identification
of carrier animals is based upon detection
of specific antibody with serological tests
while diagnosis of abortions is based upon
microscopic examination of the fetus and
immunohistochemistry.
NEOSPOROSIS
Neospora caninum
Antibody Test Kit
cELISA
Component 280-2
A. Antigen-Coated Plates 2 plate
B. Positive Control 3.6 ml
C. Negative Control 3.6 ml
D. 100X Antibody-Peroxidase Conjugate 0.3 ml
E. Conjugate Diluting Buffer 30 ml
F. 10X Wash Solution Concentrate 120 ml
G. Substrate Solution 30 ml
H. Stop Solution 30 ml
Test Kit Insert
KIT CONTENTS
1. Transfer 50 µl of samples and controls into wells
of the Antigen-Coated Plate
2. Incubate 60 minutes at room temperature
3. Wash 3 times with Wash Solution
4. Add 50 µl of Antibody-Peroxidase Conjugate
5. Incubate 20 minutes at room temperature
6. Wash 3 times with Wash Solution
7. Add 50 µl of Substrate Solution
8. Incubate 20 minutes at room temperature
9. Add 50 µl of Stop Solution
10. Read at 620-650 nm
OVERVIEW OF KIT PROCEDURE
Formula for calculating % inhibition:
% I = 100 [1-(Sample OD ÷ Negative Control OD)]
Samples producing <30% inhibition are negative. Samples
producing 30% inhibition are positive.
For the test to be valid, the mean OD of the Negative
Control must be 0.30 and <2.50. The inhibition of the
Positive Control must be30%.
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