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is a digital microscope that allows you to image living cells and tissues over long periods of time without damaging them. The space-saving and power-saving design allows it to t compactly inside a CO2 incubator, minimizing the impact on the environmental temperature. It is compatible with not only bright eld imaging but also fluorescence imaging, and the color imaging with excellent reproducibility is suitable for observing tissue sections and biological tissues.Compact Live Cell Imaging SystemCytoWatcher IIWSL-1850CytoWatcher II
1The CMOS image sensor used in CytoWatcher II has improved performance compared to conventional sensors, and is now able to take clearer images than ever before. The sensor’s light-receiving area uses a back-illuminated structure and a light-shielding structure, and the pixel size (area) has been increased by 1.6 times, improving sensitivity and saturation characteristics and suppressing noise. In addition, it uses a global shutter method (a sensor method that captures the entire subject at the same time and then outputs it), so even if you take an image of a fast-moving subject, the image will not be distorted and high-precision images that are accurate to the actual situation can be obtained. The resolution remains at 5 million pixels, but the increased pixel size makes it possible to capture a wider area of images. This makes it easier to nd and focus on your subject. Operability has been improved and clearer images can now be obtained.Digital microscope ideal for live cell imagingSpace-saving installation area of B6 sizeCytoWatcherⅡ has a moisture-resistant structure, so you can perform time-lapse imaging for a long period time even if it is installed in a CO2 incubator, which has a high humidity environment of over 95%. It uses a sensor that does not require cooling even inside the incubator, and has a power-saving design that consumes almost no power CytoWatcherⅡ is a space-saving B6 size instrument with an installation area equivalent to two 96-well plates. It is short and very compact, so it does not take up much space even when installed inside a CO2 incubator. Although it has a compact body, the stage area is large, and 10 cm dishes, T-75 asks, and 96-well plates can also be used. It is easy to carry and can be moved and used in various locations. Of course, it is also suitable for use in limited spaces such as clean benches.by switching to standby mode when not capturing. Only when capturing is the device activated, the light turns on, the camera turns on, and the image is taken. It does not become a source of unnecessary heat and minimizes the impact on the environmental temperature. Multiple devices can be installed within the incubator. Of course, even with the device inside, it does not interfere with normal cell culture environment.180 mm130 mm190 mm3Imaging while installed inside the CO2 incubator25 million pixel color CMOS sensor that captures cell dynamics1
2WSL-1850When CytoWatcherⅡ is connected to a Windows PC using a USB 3.0 cable, Power is supplied and operation can be controlled using the dedicated operation software “ImageSaver T” . Fluorescent light sources can also be used by simply connecting them to the USB terminal. After adjusting the focus and determining the observation area on the Live imaging screen, preparations are complete. All you have to do is click the start button to start imaging. It is possible to connect two CytoWatcherⅡ to one PC and take images at the same timing under the same imaging conditions. This is a convenient function when performing experiments with multiple culture conditions or comparisons with controls.CytoWatcherⅡ FL is equipped with a blue LED light source (465 nm) for excitation, a short pass lter (480 nm) for the excitation light source, and a band pass lter (525/45 nm) for uorescent imaging. Green uorescence such as GFP in living cells can be taken images over time while they are still alive. Time-lapse imaging of bright eld and uorescence images of the same cell is also possible.CytoWatcherⅡ has a wide view of 1.720 mm x 1.439 mm, making it suitable for observing tissues. Not only does it have a large eld of view thanks to its low magnication lens, but it also has higher resolution than a stereo microscope, allowing you to capture precise images. The high-performance color CMOS sensor enables color photography with high reproducibility similar to the colors of actual tissues, such as animal tissue sections and plant tissues.Color imaging with a wide view and high reproducibilityWSL-1850-BCytoWatcher IICytoWatcher II FLJust connect to the USB terminal of your PC4Compatible with uorescence imaging inside living cells56
3Easy time-lapse imaging with intuitive operationFilter holderEquipped with an orange lter, which can be pulled out and used to reduce background during fluorescence photography.Control unitControl unit for excitation LEDFLuorescent unitMoisture-proof structure with built-in excitation LED light source, excitation lter, mirror, and uorescence lterModel compatible with uorescence imagingExcitation light source: 465 nm Excitation lter: 480 nm Fluorescence lter: 525/45 nmWSL-1850-BCytoWatcher II FLwhite LED light source(for bright eld photography) Turning on/o/light intensity controlled by PC5 mega pixel color CMOS 4 x objective lenses (fixed) Digital zoom (~16x)Focus adjustment handleAdjust the focus by manually turning the handle, allowing for precise adjustments.Main body: Moisture-proof structureCan be used in high humidity environmentsWSL-1850CytoWatcher IIImaging windowThere’ s an objective lens under the windowLED light source viewed from belowDisplay images clearly with various display settings“Color/monochrome”, “grid”, “saturation detection”, and “inversion” displays make it easier to see captured images. From the settings screen, you can adjust “color/white balance”, “gain”, “sharpness”, etc.Display a grid on the image.Inverts the tone.Displays areas where the brightness is saturated in red.Switch between color/monochrome.Black and whiteGrid SaturationColourInvartGrid Saturation Invart
4Easy-to-understand settings with a simple operation screenLive imaging conditionsLighting and the intensity adjustment Brighteld: 1-63 Fluorescent: 1-127 Exposure time Range: 1 to 10,000 msCytoWatcherⅡ has two imaging modes: “Live imaging” and “Interval imaging”. After setting the cells, simply open the dedicated software “ImageSaver T” and start imaging.The list of saved images will be displayed as thumbnails. You can adjust “contrast”, “scale bar”, etc. and save it under a different name. There are three storage formats: 8bit TIFF/JPEG/BMP.Image listContrastcontrast adjustment Scale bar displaySave imageSave ascontrast adjustment Scale bar displayExport data to various storage formatsLive imagingCaptured images are displayed in real timeInterval imaging condition① Exposure time: 1 to 10,000 ms ② Shooting interval ③ Shooting period ④ Light source White LED: Bright eld photography Fluorescent LED: Fluorescent photography (Simultaneous bright eld and uorescence imaging is also possible)Interval imasingAutomatically capture and save images at regular intervalsSetting samplePlace the cells and adjust the focus while watching the live recording
516h12h10h 14h2h 8h6h4h18h 20h 24h22hImaging for various purposesFluorescence imaging of GFP-expressing cellsWound healing assayThe gure below shows an image of NIH3T3 cells taken with CytoWatcher II in live imaging mode. The area enclosed in a square will be enlarged and displayed using digital zoom.A scratch (wound) was created on conuent NIH3T3 cells using a chip, and the healing process was taken images at 10-minute intervals using CytoWatcher II. The image on the left shows images every 2 hours. You can observe the dynamic movement of cells. Please access the video using the QR code.Condition Exposure time: 10 ms Interval: 10 min Period: 1 day Lighting: White LED lighting Container: φ35 mm dish16x（Digital zoom）12x（Digital zoom）8x（Digital zoom）4xThe gure below shows images taken with CytoWatcher II FL of NIH3T3 cells 20 hours after transfection of the EGFP expression vector. The area enclosed in a square will be enlarged using digital zoom. The same cells were also imaged using interval photography 18 to 30 hours after gene transfection. If you are interested in the video, please access it using the QR code.Condition Exposure time: 1 s Interval: 20 min Period: 2 day Lighting: Exciting LED lighting Container: φ35 mm dish16x（Digital zoom）12x（Digital zoom）8x（Digital zoom）4xObservation of broblastsCondition Exposure time: 10 ms Lighting: White LED lighting Container: φ35 mm dish
6Spleen (Masson trichrome stain) Heart (Masson trichrome stain)Spleen（EvG staining）Liver (Masson trichrome stain) Intestine（HE staining）Basal ganglia (Masson trichrome staining)Expands the imaging area while maintaining the same resolutionThe figure shows images of GFP-expressing NIH3T3 cells taken with CytoWatcher and CytoWatcher II (left: 4x) and an enlarged image of the boxed area (center). Bright field images are magnified images of different fields of the same cell, similar to fluorescent images (right). CytoWatcherⅡ has an expanded shooting area of 1.6 times compared to the previous CytoWatcher. The image resolution remains the same as the previous model, 5 million pixels, but the increased pixel size allows for better sensitivity.1,425 µmCytoWatcher Ⅱ1,720 µm1,439 µmCytoWatcher1,070 µmBright eld imageFluorescent image*Enlarged bright eld image and uorescent image are different elds of view.This is dierent from conventional CytoWatcherTissue section stained imageThe gure below shows color images of various tissue sections taken using CytoWatcher II in live imaging mode. Color imaging with excellent reproducibility is also suitable for observing tissue sections.Condition Exposure time: 10 ms Lighting: White LED lightingSlide glass
ModelWSL-1850 CytoWatcher Ⅱ WSL-1850-B CytoWatcher Ⅱ FLCamera 5 mega pixels / Color CMOS camera（Global shutter method）Resolution 2448 x 2048 pixelsMagnication4x（Digital zoom：～ 16x）Field of view size 1.720 mm x 1.439 mmFocus Manual (coarse/ne movement)Light source White LED (trans-illumination)White LED (trans-illumination)Side illuminating epi-blue LED (465 nm)Filter ーExcitation: 480 nm shortpass lterEmission: 525 / 45 nm bandpass lterMoisture resistance Available at 95%RH of humidity (Usable in CO2 incubator.)SoftwareImageSaverTmageSaverT for WindowsCapture mode: Live / Time-lapseSave format: 8bit TIFF / BMP / JPEG, Color / MonochromeConnection to PC USB 3.0 × 1 USB 3.0 Type-A x1 and USB 2.0 (or 3.0) x1 OS Windows 10 / 11（64/32bit）Language Japanese/English Switchabe with ImageSaverT softwareSize, Weight 130(W) x 180(D) x 190(H) mm, 2.5 kg 130(W) x 180(D) x 190(H) mm, 2.9 kgPower source USB feeding (bus power), DC5V, 7WSpecicationWSL-1850 CytoWatcher ⅡWSL-1850-B CytoWatcher Ⅱ FLBright eld imaging Fluorescent/Bright eld imagingFluorescent imaging unitATTO CorporationHead Oce:3-2-2 Moto-asakusa, Taito-ku, Tokyo111-0041, JAPANURL: https://www.attoeng.site/