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WSE-6370/6370K LuminoGraph III LiteChemiluminescence Imaging System

Filter wheel,External light sourceCan accommodate light source and emission filter for RGB or NIR fluorescenceUV transilluminatorImaging of fluorescent stained gels (DNA or RNA, etc.)White LED transilluminatorImaging of CBB stained gels, silver stained gels※ Semi-permanent LED light that doesn’t consume UV life ※ OptionalSample trayImaging of blots (Western blots or dot blots, etc.)4 tray positions IndicatorUSB portSafety Sample tray and white LED transilluminator can be positioned according to the sample sizeCooling status, capturing status, light source status (ON/OFF)UV turn off automatically when the door is openedConnect with PC※ OptionalLuminoGraph III Lite

Simple Contrast adjustmentUser registration and file managementRegister user ID and manage imaging files individu-ally. Imaging files are saved in the logged-in user’s folder.The captured image displayed in chronological order. You can export or delete multiple images by select a date.Intuitive buttonOne-click optimal settings for fluorescent dyeEasy to capture. The menu is config-ured according to the applications.The Auto Exposure function can be applied to all applications.Select a file in Image Viewer Drag the Contrast icon Reflected on the displayed imageDragSelect Application Easy to set for Fluorescent dyeEnable Auto ExposureEasy to Use

Easily capture low-sensitivity images with Set Area functionAuto MergeMany Imaging systems that support Auto Exposure capture images basis on the strongest signal to avoid saturation. For this reason, it may be difficult to detect target protein if you capture an image with a big difference in sensitivity or the nonspecific band's signal is strong.ATTO Imaging systems, including the LuminoGraph III Lite, have Set Area function. Bands that exist in a selected area are detected. Images are saved as many as area selected.Western blot auto exposure provides Auto Merge function. Easily check the M.W. of the target protein.When capturing multiple blots, the exposure time is calculated for each specified area and each images are taken. Even the difference in the expression level is large, good results are obtained.If the target protein’s signal is weaker than other proteins such as non-specific bands, it’s difficult to detect. In this case, you can detect the target protein by setting the area.Chemiluminescence Prestained M.W.marker Merged imageFirst, capture a sample of high sensitivityCapture a sample of low sensitivity automaticallyCommon Auto Exposure Set Area Auto ExposureAuto ExposureAuto Exposure + Set AreaDifficult to detect weak signals with common Auto ExposureCan detect weak signals with Set Area function+ =Capture an image of samples at once with large differences in expression levelDetect samples with weaker sensitivityPowerful Auto Exposure

Improved performanceLuminograph IILuminograph III Lite4x4 binning, 10s2x2 binning, 10sSample : HeLa cells extract, 30ug/Lane - x 1/2 dilution1st Ab : Anti-SMAD2 Ab2nd Ab : HRP-labeled anti-rabbit IgG AbDetection reagent : WSE-7120 EzWestLumi plusPerformance is higher than our previous models. Noise is greatly reduced and sensitivity is increased. Even if you increase the binning level to detect weak signals, the device also detects the band cleanly.High sensitivityLuminograph IIILuminograph III LiteSample : HeLa cells extract, 30ug/Lane - x 1/2 dilution1st Ab : Anti-SMAD2 Ab2nd Ab : HRP-labeled anti-rabbit IgG AbDetection reagent : WSE-7120 EzWestLumi plusAdopted the lens and sensor that are the same as our high-end model. Lightweight, cost-effective, and high performance.F 0.8 lens suitable for high sensitivity imaging0.00510152025300.5F2.0F2.8F1.4F1.0F0.8F0.71.0 1.5 2.0 2.51/F2RLU x 106Theoretically, the light collection efficiency increases as the inverse of the square of the F. But, the smaller the F value, the greater the gap between the theoretical value and measured value. This is due to the effect of microlens on the surface of the sensor.The LuminoGraph III Lite features a high sensitivity F0.8 fixed focal lens, with little distortion and maxi-mum brightness.High Sensitive Imaging

Repeat IntensityFile_01.tif 39,304,149 File_02.tif 39,316,381 File_03.tif 39,290,742 File_04.tif 39,334,862 File_05.tif 39,381,957 File_06.tif 39,368,301 File_07.tif 39,338,288 File_08.tif 39,388,014 File_09.tif 39,369,365 File_10.tif 39,347,442 Average 39,343,950 SD 31,507 Uncertainty (k=2) 0.16%Powerful quantification and wide dynamic rangeSample : Standard LED (WSL-1200 KohshiFundam)Exposure : 2x2 binning, 5sA signal detection proportional to the amount of protein is required for the quantification of Western blot, and a wide dynamic range is required to detect weak signals.The right graph shows the signal measured using a reference light source emitting a faint light. You can see the linearity of R2 value over 0.999.The Luminograph III Lite has excellent lineality for the wide dynamic range, so it can be quantified simultane-ously even if the difference in the expression level is large.R² = 0.999605,00010,00015,00020,00025,00030,00035,00040,00045,0000 200 400 600 800 1,000 1,200Signal (Average)LED (pW)2.25 4.5 9 18 36 72 144 288 575 1,150Superior reproducibilityTo trust the results of the images captured, reproducibility is required that detect the same amount of signals equally.The table on the left is the uncertainty produced by repeatedly captured images using a reference light source (WSL-1200 Kohshifundam) and analyzing the intensity of the image. It has excellent reproducibility within ± 0.2% uncertain-ty.WSL-1200 KohshiFundam, our reference light source for validation, was used for reliability test.Standard light source for verification with specified luminous flux11 step variation in light volume from 1/1 to 1/1024 by PWM (Pulse Width Modulation) controlDeal with the linearity test of photometric equipmentSuitable for reproducibility testing, as the amount of light is equal even with changes in environmental temperatureWSL-1200 KohshiFundamSample : Standard LED (WSL-1200 KohshiFundam)Exposure : 2x2 binning, 10sHighly Reliable Results

Densitometry CS Analyzer 4① Drag② Automatic leveling* Actin* Target proteinQuantification of DNA gels or protein gels. Analyze bands and background automatically and calculate the intensity of bands of each lane.This software performs various analysis such as intensity measure-ment, M.W. analysis, pI analysis, and relative quantity, etc. by select-ing analysis modes according to sample types such as Lane, Spot, and Plate.It also has the ability to adjust images such as image rotation, contrast adjustment, and merge, and the adjusted images can be stored in various formats.You can save the analysis results as a CSV file that is available in MS Excel and save data in report format, including images and Standard curve, as PDF files.Quantification of regions of interesting in western blotting, culture cells, or living organisms.Quantification samples in microplate, array, or dot blotting.Spot AnalysisWe support normalization that compares the relative abundance of a specific protein of blots that are captured separately. Use CS Analyzer instead of inconveniently calculate yourself.NormalizationCan normalize 2 imagesLane AnalysisPlate AnalysisEnable easy leveling function. Draw a line which you want to make vertically by dragging a mouse.Free RotationCS Analyzer 4ATTO Densitometry Software NormalizeQuantitative Analysis

SpecificationChemiluminescentwestern blotFluorescent stained nucleic acid gelColorimetric stained protein gelFluorescent stained protein gelApplicationLuminoGraph III LiteModel WSE-6370K (Basic model) WSE-6370 (UV model)CCD Resolution 6 Mega Pixels (2750 x 2200)Pixel density 16-bit (65,536 gray levels)Camera lens F 0.8Binning 1x1 , 2x2, 4x4, 6x6Filter Motorized, 5 position (ND) Motorized, 5 position (ND / 535nm)Imaging area 4 position (10 x 7.5 cm / 14 x 10 cm / 18 x 13 cm / 26 x 20 cm)Control Windows PC (Windows 10, 32 / 64 bit) & Control SWData Output 16-bit TIFF, 8-bit TIFF, 8-bit JPEG, 8-bit BMPPower 100 - 240 VAC, 50 / 60 Hz, 200 W (MAX)Dimension 39 (W) x 46 (D) x 75 (H) cmWeight 46.6 kg 50.3 kgLightsource Epi White LED O O Trans White LED O O Trans UV X (Optional) O■ URL　http://www.atto.co.jp/　Providing research solutions for Biochemistry / Molecular Biology / Genetic engineering Head Office: 3-2-2 Motoasakusa, Taito-ku, Tokyo, 111-0041 JAPANTEL: 81-3-5827-4863 FAX: 81-3-5827-6647 Email: eig@atto.co.jpATTO CorporationSpecification