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Message T I R Z E P A T I D E2 0 2 4skindeep.nikki@gmail.comwww.skindeepnikki.comThe content in this informational guide is the intellectual property of Skin Deep beautyby nikki. This resource is for individual and personal use. Duplication & redistributionis strictly prohibited. If you have a request for distribution, contact Nikki atskindeep.nikki@gmail.com.It’s essential to conduct your own research. While the information provided here is based onextensive research tailored to my personal skincare goals, it’s important to consult a medicalprofessional for any questions or concerns you may have.10mg & 30mg – FOR WEIGHT LOSS

T A B L E O FC O N T E N T SIntr oduction to Tirzepatid eKey Benefi ts of Tirzepatide for Weight LossDosa geShou ld I O rder 1 0mg or 30mgHow to Con vert & Measure Dosage01030607How to Reconsti tuteHow to & W here t o InjectStor ageFAQ - Stor ageRisk s and Side E ffects0809101112Befo re and Afte r Photo“I R eached My Go al Weight, Now What?”Wher e to P urchas e141516How Long D oes It Take To Start Working 02

FDA approved in March 2022 as an adjunct to diet and exercise to improve glycemiccontrol in adults with type 2 diabetes ellitus, Tirzepatide injection is a glucose-dependent insulinotropic polypeptide (GIP) receptor and glucagon-like peptide-1 (GLP-1) receptor agonist. In comparative head-to-head trials, Tirzepatide surpassed Semaglutide’s HbA1creduction by an additional 24%. According to data from Phase III trial results in type 2diabetic patients, Tirzepatide was non-inferior and was superior to Semaglutide withrespect to the mean change in HbA1c from baseline to 40 weeks.At all doses, Tirzepatide (5mg, 10mg, 15mg) also outperformed Semaglutide (1mg) inbody weight reduction after 40-weeks. At 5mg, up to 80% of users achieved at least5% (15lbs) reduction in body weight; at 10mg, 45% reached a 10% (20lbs) reduction;and at 15mg (25lbs) reduction in body weight compared to Semaglutide users’ 54%,24%, and 8%, respectively.Weight loss from baseline to after 40 treatment weeks revealed that Tirzepatide (5mg,10mg, 15mg) offered at least 30%, 60%, and up to 95% greater weight loss, respectivelyvs. Semaglutide 1mg. Impact of TirzepatideAs a GIP and GLP-1 receptor agonist, Tirzepatide is a 39-amino acid modified peptidewith a C20 fatty diacid moiety that enables albumin binding and prolongs the half-life.Tirzapatide selectively binds to and activates the GIP and GLP-1 receptors, the targetsfor native GIP and GLP-1. At therapeutic doses, it acts on the brain and suppresses appetite, used inconjunction with a healthy diet and exercise. Tirzepatide helps patients adhere to areduced-calorie diet, enhancing the weight loss process. GIP is produced in the K cells of the small intestine. It binds to a specific GIP receptor,which is expressed in various tissues, including pancreatic beta cells, pancreaticalpha cells, subcutaneous and visceral adipose tissue, bone, and heart. In thepostprandial state, GIP is co-secreted with GLP-1, and they appear to interact inan additive fashion to potentiate glucose-induced insulin secretion. However, GIPexhibits different effects than GLP-1 on glucagon secretion. In the euglycemic orhypoglycemic states, GIP enhances glucagon activity.Dual acting GLP-1 and GIP Receptor Agonist - TirzepatideA synthetic dual-acting GIP and GLP-1 receptor agonist (Tirzepatide)is available for the treatment of hyperglycemia in patients with type 2diabetes]. The effect of Tirzepatide is largely mediated by its GIPcomponent. Tirzepatide has a half-life of five days, allowing for once-weekly administration.I N T R O D U C T I O N T O T I R Z E P A T I D E

Benefits of Weight Loss:o Better sleepo Reduce cancer risko Improved sex driveo Prevent Type 2 diabeteso Arthralgia – Reduce join paino Hypertension – Lowers Blood Pressureo Balanced moodo Decreased risk of strokeo Decrease High triglycerideso Higher self-esteemo Hypercholesterolemia – Lowers LDLo More energyo A healthier hearto Better Moodo Improved immunityo Improve Sleep apneao Clearer skino Food will taste bettero Improved mobility and reduced paino Increasing mobilityo Reduce your health riskso Reduce sexual dysfunctionK E Y B E N E F I T S O F T I R Z E P A T I D EF O R W E I G H T L O S S0 1

Tirzepatide, the active ingredient in medications like Mounjaro and Zepbound, startsworking in your body fairly quickly after injection. However, it may take some timebefore you notice significant, measurable effects, especially for weight loss. Here's a breakdown of what to expect:Initial effects (within hours to days): Tirzepatide begins to influence hormones thatregulate appetite and blood sugar control shortly after your first dose. You mightnotice a decrease in appetite or feel fuller after eating more quickly.Noticeable changes (within weeks to months): You might start seeing moresignificant weight loss or improvements in blood sugar levels around the 4-weekmark, as the medication reaches consistent levels in your body. Clinical trials showthat the greatest weight loss generally occurs within the first 8 to 9 months oftreatment.Full effects (4-6 months): If you're using Mounjaro for blood sugar control, the fulleffects on lowering blood glucose levels typically peak around 4 to 6 months afterstarting treatment.Long-term results: Tirzepatide is intended for long-term use. Continued use isgenerally recommended to maintain weight loss and blood sugar improvements.Clinical trials showed that patients using the highest dose of Zepbound forapproximately 16.5 months lost an average of 15% to 21% of their starting bodyweight. Factors influencing how quickly it works: Dosage: Your doctor will start you on a low dose and gradually increase it over timeto help your body adjust and minimize side effects. This titration process can takeseveral months to reach the optimal dose for you.Individual response: Everyone's body responds differently to medication. Somepeople may see quicker results than others.Lifestyle changes: Adopting a healthy diet and engaging in regular physical activitycan enhance the effects of tirzepatide and contribute to faster results.Underlying health conditions: Your overall health, including whether you have type2 diabetes and how well controlled it is, can impact the effectiveness of themedication.Consistency: It's crucial to take your weekly injections consistently as prescribed tomaximize effectiveness. Important note: It's essential to follow your healthcare provider's instructions regardingdosage and treatment plan. Don't be discouraged if you don't see rapid weight loss inthe initial weeks; consistent effort and adherence to the plan will lead to better resultsover time. If you have any concerns about your progress or side effects, don't hesitate to consultyour doctor or a registered dietitian.H O W L O N G D O E S I T T A K E T OS T A R T W O R K I N G ?0 1

Tirzepatide – 10mg VialDosage and Administration: (Dilute 10mg Vial with 2ml BAC Water, using a 1ml insulin syringe) Month 1: 2.5mg once weekly (.5ml or 50th line) = 1 – 10mg Vials per monthMonth 2: 5mg once weekly (100th line) = 2 – 10mg Vials per monthMonth 3: 7.5mg once weekly (150th line) = 3 – 10mg Vials per monthMonth 4: 10mg once weekly (200th line) = 4 – 10mg Vials per monthMonth 5: 12.5mg once weekly (250th line) = 5 – 10mg Vials per monthMonth 6: 15mg once weekly (max dose) (300th line) = 6 – 10mg Vials per month Or Dr. William Seed recommendation (just a side note)· Inject 2.5mg SubQ - 7d x 4 weeks· Inject 3.5mg SubQ - 7d x 4 weeks (at 7-week, determine if needs to increase or hold here)· Inject 5.0mg SubQ D O S A G E0 3Injectable Tirzepatide can be administered with or without foodIf you need to change the day of the week, ensure the previous dose wasgiven 2 or more days before.Missed dose: Administer as soon as possible within 4 days (96 hours); ifmore than 4 days have passed, skip dose, and administer next dose onscheduled day. In each case, patients can resume their regular onceweekly dosing schedule.It is important that you do your own research. The information provided inthis document was attained by countess hours of research but used formy health issues and goals. Please seek medical advice from aprofessional regarding any questions and concerns you may have. Many patients report a suppressed appetite after their first dose, but somepatients don't experience this benefit until they've taken Tirzepatide for amonth or more.

What is Titration? And Why Do We Use It?In medical terms, titration refers to the process of adjusting the dosage of amedication based on an individual's response to treatment. This involvesstarting with a low dose and gradually increasing it until the desiredtherapeutic effect is achieved while minimizing side effects. Dose titration is recommended with tirzepatide to improve tolerability. In otherwords, you give your body a little bit of time to get used to it. Starting with alower dose also allows healthcare providers/you to assess your response tothe medication and minimize the risk of adverse effects.In the case of tirzepatide, you may titrate up monthly, typically increasing thedose every four weeks until you reach the optimum dosage for your body. Thiscould be anywhere from 5 mg to 15 mg, depending on how your body reacts.However, as stated above, most patients reach their optimal level at 5mg or 7.5mg.D O S A G E - 1 0 M G0 4

D O S A G E - 3 0 M G0 5

S H O U L D I O R D E R 1 0 M G O R 3 0 M G ?06 If you are just starting your weight loss journey the 10mg vial would be best because youwill need the following: 1st month - 1, 10mg Vial2nd month - 2, 10mg Vial After you reconstitute a vial, it is best if it only stays in the refrigerator for approx. 30 days. Ifyou are not on the higher dose the 30mg vial might be too large and by the end of the 30days, the efficacy will start to lessen. Once you reach the 7.5mg weekly shot, typically by the 3rd month, the best bang for yourbuck would be the 30mg vial because you will be able to use the entire bottle in about 30days. However, with the volatile Tirzepatide market, these 2 options might not both be availableat the same time. Tirzepatide Dosage and Administration Comparision(Dilute Tirzepatide 10mg Vial with 2ml BAC Water, using a 1ml syringe) · Month 1: 2.5mg once weekly (50th line on 1ml syringe) = 1 – 10mg Viala per month· Month 2: 5mg once weekly (100th l line on 1ml syringe) = 2 – 10mg Vials per monthAfter your 2nd month, if you choose to increase your dosage, if Tirzepatide 30mg vial isavailable it is best to use this vial because you will save $· Month 3: 7.5mg once weekly (150th line on 1ml syringe) = 3 – 10mg Vials · Month 4: 10mg once weekly (200th line on 1ml syringe) = 4 – 10mg Vials per month· Month 5: 12.5mg once weekly (250th line on 1ml syringe) = 5 – 10mg Vials per month· Month 6: 15mg once weekly (max dose) (300th line on 1ml syringe) = 6 – 10mg Vials permonth (Dilute Tirzepatide 30mg Vial with 2ml BAC Water using a 1ml syringe)If you are at the lower dosage, 30mg vial might be too large and by the end of the 30 days,the efficacy will start to lessen. · Month 1: 2.5mg once weekly (16.7th line on 1ml syringe) = 1 – 10mg Vial per month· Month 2: 5mg once weekly (33th line on 1ml syringe) = 2 – 10mg Vials per month· Month 3: 7.5mg once weekly (50th line on 1ml syringe) = 3 – 10mg Vials per month (or 1-30mg Vial)· Month 4: 10mg once weekly (66.7th line on 1ml syringe) = 4 – 10mg Vials per month· Month 5: 12.5mg once weekly (83.3th line on 1ml syringe) l = 5 – 10mg Vials per month· Month 6: 15mg once weekly (100th line on 1ml syringe) (max dose) = 6 – 10mg Vials permonth

The mg must be converted intomcg for this Peptide Calculator. https://www.rapidtables.com/convert/weight/mg-to-mcg.htmlExample for Week 1 Dosage: Needto convert 2.5mg to mcg for thePeptide Dosage ConverterCalculator:H O W T O C O N V E R T & M E A S U R ED O S A G E0 7Additionally, tools like aPeptide Calculator canassist in precisemeasurements,contributing to the overallhandling and stability ofpeptides in variousapplications. PeptidesDosage Calculator:https://peptidecalc.com/

H O W T O R E C O N S T I T U T E08Using Bacteriostatic Water as the diluent:Step 1 – Remove the plastic cap from the 10mg peptide vialto expose the rubber stopper.Step 2 – Remove the plastic cap from the BAC water vial toexpose the rubber stopper.Step 3 – To prevent bacterial contamination, swab therubber stoppers with alcohol.Step 4 – Extract 2ml (milliliters) of BAC water from the vial.Step 5 – Insert the 2ml (milliliters) of BAC water into thepeptide vial, letting the water slowly enter the vial.Step 6 – Gently swirl the solution until all peptides aredissolved – do not shake the vial.To reconstitute a peptide, watch here:https://www.youtube.com/watch?v=T00QEDDm8H8

H O W T O & W H E R E T O I N J E C T0 9Where to Administer:Tirzepatide is administered subcutaneously (SubQ) into thefatty tissue just beneath the skin, typically in the abdomenarea. Researchers are advised to rotate the injection sitewith each administration.

Storage of peptides in solution:When preparing stock solutions, it’s recommended to use dry (anhydride)organic solvents to prevent premature hydrolysis. The stability of peptidealiquots can vary, lasting from weeks to months. A widely acceptedguideline is that peptides in solution remain generally stable for 1-2 weeksat +39°F, 3-4 months at -4°F, and up to 1 year at -112°F. Understandingthese considerations is vital for researchers.We recommend storing peptides at < -4 °F or -112°F for a period longerthan 4 months.More Storage Information: https://www.slimtexasmed.clinic/post/does-tirzepatide-need-to-berefrigeratedS T O R A G E1 0https://a.co/d/3MQJdBASuggested Storage Refrigerator forPeptides, Makeup and Serums .

Are peptides supposed to be refrigerated?Peptides are generally recommended to be stored in a cool anddry environment, but refrigeration is not always necessary. Itdepends on the specific peptide and its stability characteristics.Always refer to the supplier’s guidelines for storagerecommendations.At what temperature are peptides stable?The stability of peptides varies, but they are often stable attemperatures ranging from -4°F to +39°F (-20°C to +4°C).However, this is a general guideline, and it’s crucial to check thespecific stability information provided by the supplier for eachpeptide.Can I store peptides in the freezer?Yes, peptides can be stored in the freezer, and it is a commonpractice to enhance their shelf life. Peptides are often stable forlonger periods when stored at temperatures below -4°F (-20°C).Again, check the specific storage recommendations provided bythe supplier.Are peptides heat sensitive?Peptides can be sensitive to heat, and exposure to hightemperatures may lead to degradation. It is advisable to storepeptides in a cool environment and avoid prolonged exposure toelevated temperatures. Always follow the recommended storageconditions to maintain the stability and integrity of the peptides.F A Q - S TO RA GE1 1

Tirzepatide: All of the information above re: GLP-1 receptor agonists applies toTirzepatide as well as Semaglutide, as the risks, benefits and side effects aresynonymous; but here are some specifics from Mounjaro’s (Tirzepatide’s)website. Considerations: In both male/female rats causes dose dependent thyroid C-cell tumors Unknown whether Mounjaro (Tirzepatide) causes thyroid C-cell tumorsincluding medullary thyroid carcinoma (MTC) in humans Mounjaro (Tirzepatide) is contraindicated in patience with personal or familyhistory of MTC or in patience w/MEN 2 (multiple endocrine neoplasia type 2) Risk of Pancreatitis ➢ Do baseline labs Lipase/Amylase ➢ Repeat labs every 3 months ➢ If symptoms of Pancreatitis DC Rx refer to your medical provider ➢ If slightly elevated and asymptomatic evaluate and use clinical judgment ➢ Exacerbated with alcohol use; discourage pt’s from excessive drinking Risk of hypoglycemia w/ concomitant use of insulin secretagogues or insulin Acute kidney injury related to gastrointestinal conditions secondary todehydration have been reported Associated with gastrointestinal adverse effects Diabetic retinopathy complications in patients with history of diabeticretinopathy Acute gallbladder disease reported by 0.6% users in a study Drug interactions- potential impact on medications as it delay’s gastricemptying Pregnancy- Limited data. Based on animal studies there may be risks to thefetus; use only if potential benefit justifies the potential risk to the fetus R I S K S & S I D E E F F E C T S1 2Some have experience muscle and hair loss due to losing weight to quickly.Make sure you are getting the proper nutrients and incorporating weighttraining into your routine.

Clinical Pearls- Tirzepatide: Same as above for GLP-1 section We have seen Tirzepatide work better than Semaglutide for weight loss insome patients, as it should on paper; but there are some people who respondbetter to Semaglutide as well. Generally we can tell by the first few doses(2.5mg, 3.5mg and 5mg) ➢ For our patients that are looking to lose > 30lbs we encourage Tirzepatide (though we have had patience lose > 30lbs with Semaglutide as well) ➢ For the who only need to lose 10-15 lbs, we have found Tirzepatide to be too aggressive at times, Semaglutide is recommended ➢ For the patients who need between 20-30 lbs weight loss, our decision is subjective based on all our information (H&P, BCA, Labs, medications, lifestyle, financial consideration) Daily drinkers (2-3 glasses wine/evening) have struggled loosing Sedentary patients unwilling to exercise stuggle with muscle loss Patient’s w/ gut inflammation (constipation, bloating, distended, poor diets) For the best results they need to have a healthy gut; address the microbiome Prebiotic (more important than a probiotic) every day can be beneficialR I S K S & S I D E E F F E C T S1 3

B E F O R E & A F T E RI lost 40lbs with Tirzepatide in 4 months and have kept it off by stayingon the lowest dosage that works for my appetite cravings. 1 4

Now that I am at my goal weight, how am I maintaining the weight loss?I initially went off Tirzepatide but started getting hungry and noticed I was snackingagain. I found the benefits of weight loss outweigh the idea to going off the peptide. Idecided to take the mindset of peptide tapering and holistic health. Here is what Idid:1.After I went off, I went back on at the lowest dose.a.I recommend tapering down until you find the lowest dose that keep you incontrol. 2.Protein Shakes every day! My goal is 100g of protein a day.3.YOGA! I started online with literally 10 minutes a day. I am now a certified yogainstructor and am obsesses with yoga. (I am going to start my own yogaYouTube channel soon). Here is a great channel to start your yoga journey:https://www.youtube.com/user/yogawithkassandra4.I have now incorporated weight training into my weekly routine. I am starting with2x a week and hopefully working up to 3x.The bottom line: Listen to your body. The goal is not to be “skinny” but to be healthy!Make sure to take good care of your body…we only have one in this lifetime. Here are more ideas to think about:Titrate down to a maintenance load of Weight Loss Peptide and/or stretch out thenumber of days between injections and keep on taking it.With the success these peptides/medications, I am sure that Pharma Companiesare working on another medication that will be taken after the weight loss goal isachieved to help maintain.Exercise: Incorporate moderate-intensity exercise into your routine and try tomake gradual lifestyle changes. You can start with walking and then add otheractivities like running, rowing, or strength training.Diet: Eat a plant-forward diet that includes lean proteins, fruits, and vegetables.You can also try adding protein to meals throughout the day and eating foodsthat are high in fiber and healthy fats, like whole grains, leafy green vegetables,and avocados.Other habits: Prioritize sleep and try to get 7 to 9 hours each night. You can alsotry to manage stress and hold yourself accountable by monitoring your progress.Here is an interesting article: https://www.healthline.com/health-news/slowly-coming-off-ozempic-wegovy-may-prevent-rebound-weight-gainR E A C H E D G O A L W E I G H T ~N O W W H A T ?1 5

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W H E R E T O P U R C H A S ESHOPPEPTIDESW W W . S K I N D E E P N I K K I . C O M1 7PRECISION PEPTIDE CO.Code: PEP5 (5% OFF)https://precisionpeptideco.com/ref/6/FREE SHIPPING ON ALL ORDERS OVER $100 (US only)FREE 2ml Bacteriostatic Water with every purchase!4.8 on Trustpilot Sign up for SKIN DEEP Peptide Email List to be notified of Monthly Sales.Email: skindeep.nikki@gmail.comAverage Cost of Precision Peptides for 1 – Tirzepatide 10mg$110 + 1 FREE Bacteriostatic water per order + FREE Shipping over $100Cost per Vial: $110 – (5% with PEP5) = $104.50

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W W W . S K I N D E E P N I K K I . C O MWant more info on Peptides?www.skindeepnikki.com/peptides