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Easy Accurate Compact Economy High performance precast gel c PAGEL HR In a short time e PAGEL HR In high Speed and Resolution e PAGEL Economy A ordable price p PAGEL For low molecular weight peptide separation PAGEL For high molecular weight m PAGEL For multiple samples

Principle Polyacrylamide gel electrophoresis Gel electrophoresis is a basic method for separating and analyzing proteins and nucleic acids In particular PAGE polyacrylamide gel electrophoresis has been used for basic research of proteins and nucleic acids ATTO is one of the rst companies to produce pre cast gels for PAGE which is ATTO high speed resolution electrophoresis Electrophoresis apparatus the most important in gel electrophoresis and has over 30 years of experience in this eld Our pre cast gels have excellent separation reproducibility and storage stability etc In recent years the quality has been improved to enable Reagents pre cast gel High speed resolution electrophoresis Polyacrylamide gel electrophoresis of proteins SDS PAGE SDS Polyacrylamide gel electrophoresis SDS PAGE Pr o t e i n s an d p e p t i d e s ar e c h ar g e d e i t h e r o r d e p e n d i n g on the content of each amino acid and the pH of the electrode buffer SDS sodium dodecyl sulfate anionic surfactant is bound to the protein transiently charge it to and move to side At this time the protein is separated in the polyacrylamide gel by the molecular sieving effect of the gel showing mobility according to the molecular weight The molecular weight cut off range can be varied by changing the gel concentration In the most common Laemmli method Tris Glycine as the gel bu er and TrisHCl as the electrode bu er are used but Tris Tricine bu er can also be used for low molecular weight range Non denatured polyacrylamide gel electrophoresis Native PAGE Non denaturing or native has various meanings but it usually refers to a method in which proteins are not reduced SDS is not added and no heat is added Normally it refers to a method in which more natural proteins are used as samples Polyacrylamide gel electrophoresis of DNA Samples SDS process SDS reductant heat Load samples Turn on the power High speed resolution electrophoresis is an electrophoresis method that combines high speed separation and resolution which can be achieved through high in quality of pre cast gels reagents and electrophoresis apparatus Nucleic acids DNA RNA are negatively charged from phosphate residues due to their composition and move to the anode side by electrophoresis In a polyacrylamide gel like proteins the molecular sieving e ect of the gel causes the separation to show migration distance according to the molecular weight For nucleic acids agarose gel is often used but for DNA of 100 kbp or less polyacrylamide gel enables to get sharper bands and better separation The high resolution of the gel was also used for base sequence determination Since the gel is transparent and thin stain de stain are quick and the background is clean Examples of actual electrophoresis pattern ATTO pre cast gel PAGEL series can be used for SDS PAGE Native PAGE of protein PAGE of DNA etc SDS PAGE of protein Protein and DNA EzStandard 1 174 Hinc PAGE of DNA

PAGEL series PAGELR list PAGEL list Please select PAGEL according to the gel sizes purpose of electrophoresis number separation of samples operation time etc Product name c PAGEL HR p PAGEL Size Gel size 60 W 60 L mm 0 75mm thickness Compatible apparatus Compact PAGE series Page No p 3 4 Type Gel concentration 5 types Glass plate size 76 W 70 L mm Tricine based for low molecular weight Gel bu er Tris HCl 7 5 10 12 5 15 5 20 16 5 15 samples CHR7 5L CHR10L CHR12 5L CHR15L CHR520L CP16 5S For 2D electrophoresis CHRD7 5L CHRD10L CHRD12 5L CHRD520L 7 L 2331615 without wells 2331685 2331625 2331655 2331635 2331665 Product name 2331645 2331605 2331695 2331675 e PAGEL HR Size Gel size 90 W 83 L mm 1mm thickness Compatible apparatus Mini slab size electrophoresis PageRun series Page No p 5 10 Type Gel concentration types Gel bu er Tris HCl 7 5 10 12 5 15 10 20 5 20 14 samples EHR T7 5L EHR T10L EHR T12 5L EHR T15L EHR T1020L EHR T520L 18 samples EHR R7 5L EHR R10L EHR R12 5L EHR R15L EHR R1020L EHR R520L 24 L 2331950 18 L 2332050 2331955 2332055 2331960 2332060 Product name Size 2331965 2332065 2331975 2331970 2332075 2332070 e PAGEL Gel size 90 W 83 L mm 1mm thickness Glass plate size 120 W 100 L mm Compatible apparatus Mini slab size electrophoresis PageRun series Page No p 11 16 Type Glass plate size 120 W 100 L mm Gel concentration types Gel bu er Tris HCl 7 5 10 12 5 15 10 20 5 20 14 samples E T7 5L E T10L E T12 5L E T15L E T1020L E T520L 18 samples E R7 5L E R10L E R12 5L E R15L E R1020L E R520L For 2D electrophoresis E D7 5L E D10L E D12 5L E D520L 24 L 2331800 18 L 2331700 without wells Product name 2331940 2331810 2331710 2331910 p PAGEL Size Gel size 90 W 83 L mm Glass plate size 120 W 100 L mm Compatible apparatus Mini slab size electrophoresis PageRun series Page No p17 18 Type Tricine based for low molecular weight 16 5 14samples 18samples 12 samples 28 L P T16 5L 2332260 P R16 5L 2332265 2331820 2331850 2331720 2331750 2331920 Product name Size 2331840 2331740 2331830 2331730 2331930 PAGEL Gel 120 W 83 L mm Glass plate 160 100mm Compatible apparatus Multiple lane gel electrophoresis Page No p 21 22 Type Gel concentration 5 20 30 samples 18 L well M 520L 2332240 2

c PAGELR HR Compact size High performance compact size approx 6 6cm pre cast gel Upgraded High speed resolution electrophoresis TM Electrophoresis time in a minimum of 10min CHR T520L c PAGEL HR For 15 samples Green comb Features Upgraded version of c PAGEL Pre cast gel for polyacrylamide gel electrophoresis available for high speed resolution electrophoresis High speed separation 10min High resolution separation Available for wide range application For SDS PAGE Native PAGE DNA etc Screening con rmation of expression Student training western blotting Improved gel in quality High resolution Sharp bands especially separation ability in low molecular range improved Smiling less patterns Minimize deformation of gradient gel Longer expiry date 1year Improved e ciency of western blotting Please visit our website to check the videos This product can be used in the same way as c PAGEL Operation overview Pre cast gel or hand cast gel Fill with electrode bu er to the upper chamber Load samples Preparation Fill with electrode bu er EzRun EzRunMOPS etc Set the gel Fix with a plate holder Electrophoresis pattern De stain by water Set the power supply after closing the safety cover 3 Detection time If you use a microwave the bands Stain De stain the gel can be seen in approx 10 min use EzStainAQua etc Approx 30 min at room temperature Turn on the power Connect the AC code Take out the plate and gel Select the mode and start after electrophoresis electrophoresis by pressing Run Electrophoresis time High mode approx 10min Standard mode approx 30min

Compact size Specifications Model name code CHR c PAGEL HR Sample wells Comb CHRD c PAGEL HR 15 wells None 2mm width maximum loading volume approx 7 L Gel size None 60mm W 60mm L Gel plate Made of glass model 16 5 Polyacrylamide gel Polyacrylamide gel code Gel concentration model code 7 5 CHR7 5L 2331615 7 5 CHRD7 5L 2331685 10 CHR10L 2331625 10 CHRD10L 2331655 12 5 CHR12 5L 2331635 12 5 CHRD12 5L 15 CHR15L 2331645 15 2331665 5 20 CHR520L 2331605 5 20 Gel concentration The molecular weight range Total 4 8mm thickness Polyacrylamide Tris HC Gel concentration Product code 2mm width Polyacrylamide gel 7 5 10 12 5 15 consistent Gel principal component 15 wells For 2D electrophoresis 0 75mm thickness 76mm W 70mm L 5 20 Gradient Gel concentration cp PAGEL CHRD520L 2331675 Polyacrylamide Tris Tricine C16 5S 2331695 c PAGEL is basically made to order other than CHRD520L The molecular weight range Protein Nucleic acid 7 5 40 400 kDa 200 3000 bp 10 20 300 kDa 100 2000 bp 12 5 10 250 kDa 70 1800 bp 15 2 200 kDa 50 1500 bp 5 20 5 400 kDa 30 2500 bp 1 75kDa 1000 75 000Da 1 year when stored in a refrigerator NOT to be frozen Expiry date Package 10 plates per box Refrigeration at 5 10 is recommended for storage If stored in a cold air outlet or below 4 the gel will freeze and become unuseable Can be transported in room temperature short time The molecular weight range of protein separation is based on electrophoresis which is used bu er of EzRun 25mM Tris 192mM glycine 0 1 SDS The molecular weight range of DNA separation is based on electrophoresis which is used bu er of EzRunTG 25mM Tris 192mM glycine The migration varies depending on the component of the electrophoresis bu er EzRunMOPS electrode bu er is recommended for high speed electrophoresis of CHR EzRunMOPS cannot be used for cp PAGEL electrophoresis For low molecular weight CP16 5S cp PAGEL High speed electrophoresis minimum 15min Wide range of molecular weight 1000Da 75kDa Sharp bands Long shelf line 1 year EzRunT tricine based electrode bu er is used Please refer to p PAGEL p17 18 for electrophoresis pattern Data High speed electrophoresis SDS PAGE Clear patterns can be obtained with c PAGEL HR even in high speed electrophoresis Electrophoresis apparatus WSE 1010 CompactPAGE Ace High mode M W 245 000 75 000 10min for 1 gel Sample Molecular weight marker AE 1440 EzStandard WSE 7020 EzProtein Ladder etc Gel c PAGEL HR 5 20 polyacrylamide gel pre cast gel 60 60mm Electrode bu er AE 1440 EzRun Tris Gly SDS CBB staining AE 1340 EzStain AQua CBB staining solution Electrophoresis of DNA Native PAGE are available with c PAGEL HR 25 000 10 000 Compatible apparatus Actual size WSE 1010 cPAGE Ace WSE 1025 cPAGE Ace Twin 4

e PAGELR HR Mini size High performance mini size approx 9 8cm pre cast gel Upgraded High speed resolution electrophoresis TM Electrophoresis time in only 20 min EHR T520L e PAGEL HR For 18 samples green comb 14 samples orange comb Features High speed separation within 20min High resolution separation A wide range of application For SDS PAGE Native PAGE DNA PAGE etc Improved gel quality High resolution Sharp bands especially separation ability in low molecular range improved Smiling less patterns Minimize deformation of gradient gel Longer expiry date 1year Improved transfer e ciency of western blotting e PAGEL HR features e PAGEL HR pattern Blotting Electrophoresis Deformation resistant gel Minimize smiling Run in straight lane High molecular weight area Low molecular weight area Blue line Sharp bands in both high and low molecular weight range with high blotting e ciency Dotted line Red line Sharp bands in low molecular range Green line CBB staining of blotted membrane 5 20 gradient gel Electrophoresis pattern Please visit our website to check the videos Operation overview Set the gel to electrophoresis chamber Open the package of e PAGEL HR e PAGEL HR pre cast gel and PageRunAce 5 Load samples Fill with electrode bu er Start electrophoresis Set the power supply Connect the AC code Running time High mode approx 25min Standard mode approx 70min

Mini size Specifications Model Name code EHR T e PAGEL HR EHR R e PAGEL HR Gel size 90mm W 83mm L Plate size Sample wells 120mm W 100mm L Expiry date Package 18 wells 2 9mm width maximum loading volume 18 L 5 20 10 20 gradient polyacrylamide gel 7 5 10 12 5 15 consistent Model Principal materials Total 5mm thickness 14 wells 4 2mm width maximum loading volume 24 L Gel concentration The molecular weight range 1mm thickness Code Number of wells samples EHR T7 5L 2331950 14 EHR R7 5L 2332050 18 EHR T10L 2331955 14 EHR R10L 2332055 18 EHR T12 5L 2331960 14 EHR R12 5L 2332060 18 EHR T15L 2331965 14 EHR R15L 2332065 18 EHR T520L 2331970 14 EHR R520L 2332070 18 EHR T1020L 2331975 14 EHR R1020L 2332075 18 Gel concentration polyacrylamide gel The molecular weight range Protein Nucleic acid 7 5 40 400 kDa 200 3000 bp 10 20 300 kDa 100 2000 bp 12 5 10 250 kDa 70 1800 bp 15 2 200 kDa 50 1500 bp 5 20 5 400 kDa 30 2500 bp 10 20 2 300 kDa 30 2000 bp Gel polyacrylamide Plate glass Comb PP mold 1 year when stored in a refrigerator NOT to be frozen 10 plates per box Please place an order of pre cast gel PAGEL type of ATTO Page Run Ace Mini Slab and mPAGE series for electrophoresis apparatus p 7 Refrigeration at 5 10 is recommended for storage If stored in a cold air outlet or below 4 the gel will freeze and become unusable Can be transported in room temperature short time The molecular weight range of protein is based on electrophoresis which is used bu er of EzRun 25mM Tris 192mM glycine 0 1 SDS The molecular weight range of DNA is based on electrophoresis which is used bu er of EzRunTG 25mM Tris 192mM glycine The migration varies depending on the component of the electrophoresis bu er EzRunMOPS electrophoresis bu er is recommended for high speed electrophoresis 6

e PAGEL HR R Mini size Compatible apparatus WSE 1150P PageRunAce AE 6530P mPAGE WSE 1165 Mini slab size Mini PAGE system with a builtin power supply high speed Gel quantity 2gels max Bu er volume approx 500mL Gel quantity 2gels max Bu er volume approx 500mL Gel quantity 2gels max Bu er volume 400 650mL Electrophoresis data High speed electrophoresis data of e PAGEL HR High speed electrophoresis EzRunMOPS Clear patterns can be obtained with e PAGEL HR even in high speed electrophoresis Gel EHR T520L e PAGEL HR 5 20 Electrode bu er EzRun MOPS High speed electrophoresis PageRunAce Output C W 24W Running time 20min EzRunMOPS is recommended for detection of a wide range of bands In addition high voltage electrophoresis of 250V can be completed in a minimum of 20 minutes 24W constant power 20min High speed electrophoresis EzRun Standard electrophoresis EzRun Gel EHR T520L e PAGEL HR 5 20 Electrode bu er EzRun tris glycine SDS High speed electrophoresis Output C V 300V gel Running time 30min Standard electrophoresis Output C C 20mA gel Running time 75min 7 Constant voltage 300V 30min Constant current 20mA 75min

Mini size Comparison of electrophoresis patterns of e PAGEL HR and e PAGEL The gure below shows the results of separating the same sample with 5 20 gradient gel of e PAGEL HR and e PAGEL By using e PAGEL HR electrophoresis is completed in 30 minutes which is half the time of e PAGEL and wider range of molecular weight smiling less patterns and clearer data can be obtained e PAGEL HR e PAGEL The figure on the left is a comparison of lanes of the EzProtein Ladder The greatest advantage of the 5 20 concentration gradient gel has the much wider range of molecular weight By using e PAGEL HR the molecular weight range between high and low molecular weig h t ge ts eve n wid er t han th at of e PAGEL 245 180 140 100 75 60 45 35 25 20 15 5 kDa 26 6 17 0 14 2 6 5 e PAGEL HR e PAGEL 100 10 Expansion of separation range on the low molecular weight area 1 0 The figure on the right shows the results of migration distance analysis of EzProtein Ladder e PAGEL HR has better linearity indicating that the separation range of low molecular weight area is expanded 10 e PAGEL HR 1000 e PAGEL The left figure shows comparison of peptide marker lanes The 3rd and 4th bands from the top and the 5th and 6th bands are clearly separated with e PAGEL HR 50 Migration distance 100 Features of e PAGEL HR Especially a wider separation range of low molecular weight range More clear bands can be obtained 3 5 1 0 kDa DNA electrophoresis data of e PAGEL HR EHR T7 5L e PAGEL HR DNA Gel EHR T7 5L 7 5 14wells gel Sample DNA molecular weight marker etc Electrode bu er AE 1410 EzRun TG 25mM tris 192mM glycine Electrophoresis apparatus AE 6530 mPAGE Power Constant current 20mA 60min Staining WSE 7130 EzFluoroStainDNA Fluorescent staining Detection VariRays Blue LED Exposure time 0 5 sec DNA electrophoresis is also available by using e PAGEL HR ePAGEL HR TBE hand cast gel Molecular weight bp 1500 1000 900 800 700 600 500 400 Please refer to the data of e PAGEL for the electrophoresis pattern and migration distance of DNA at each gel concentration 300 200 8

e PAGEL HR R Mini size Electrophoresis patterns EHR T7 5L e PAGEL HR High speed mode SDS PAGE Gel EHR T7 5L 7 5 14wells gel Sample AE 1440 EzStandard molecular weight marker etc Electrode bu er AE 1410 EzRun 25mM tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6530 mPAGE Power Constant voltage 300V 30min Staining AE 1340 EzStainAQua CBB staining EHR T10L e PAGEL HR SDS PAGE Gel EHR T10L 10 14wells gel Sample AE 1440 EzStandard molecular weight marker etc Electrode bu er AE 1410 EzRun 25mM tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6530 mPAGE Power Constant voltage 300V 30min Staining AE 1340 EzStainAQua CBB staining Molecular weight Da 200 000 97 200 66 400 45 000 Molecular weight Da 200 000 97 200 66 400 45 000 29 000 20 100 EHR T12 5L e PAGEL HR SDS PAGE Gel EHR T12 5L 12 5 14wells gel Sample AE 1440 EzStandard molecular weight marker etc Electrode bu er AE 1410 EzRun 25mM tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6530 mPAGE Power Constant voltage 300V 30min Staining AE 1340 EzStainAQua CBB staining Molecular weight Da 200 000 97 200 66 400 45 000 29 000 20 100 14 300 EHR T15L e PAGEL HR SDS PAGE Gel EHR T15L 15 14wells gel Sample AE 1440 EzStandard molecular weight marker etc Electrode bu er AE 1410 EzRun 25mM tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6530 mPAGE Power Constant voltage 300V 30min Staining AE 1340 EzStainAQua CBB staining 9 Molecular weight Da 200 000 97 200 66 400 45 000 29 000 20 100 14 300

Mini size EHR T520L e PAGEL HR SDS PAGE Gel EHR T520L 5 20 14wells gel Sample AE 1440 EzStandard molecular weight marker etc Electrode bu er AE 1410 EzRun 25mM tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6530 mPAGE Power Constant voltage 300V 30min Staining AE 1340 EzStainAQua CBB staining EHR T1020L e PAGEL HR SDS PAGE 200 000 97 200 66 400 45 000 29 000 20 100 14 300 Molecular weight Da Gel EHR T1020L 10 20 14 wells gel Sample AE 1440 EzStandard molecular weight marker etc Electrode bu er AE 1410 EzRun 25mM tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6530 mPAGE Power Constant voltage 300V 30min Staining AE 1340 EzStainAQua CBB staining Related products Molecular weight Da 200 000 97 200 66 400 45 000 29 000 20 100 14 300 Please refer to the end of this booklet for more information of ATTO Ez series reagents Sample preparation AE 1430 EzApply Sample preparation kit for SDS PAGE Sample preparation bu er for SDS PAGE reduction or non reduction can be selected since DTT with strong reducing power is in a separate container Electrode bu er AE 1410 EzRun AE 1411 EzRun Electrode bu er for SDS PAGE Tris glycine SDS electrophoresis bu er for SDSPAGE Dissolve powder for 10L with distilled water for use of AE 1410 AE 1411 is solution type no preparation required Electrode bu er WSE 1410 EzRun MOPS Electrode bu er for high speed resolution SDS PAGE MOPS Electrode bu er for SDS PAGE A wide molecular weight range available for high speed electrophoresis Please refer to p 16 for electrophoresis data Compact size power supply AE 8155 35 myPower 500 300 AE 8155 output setting Voltage 1 500V Current 1 200mA AE 8135 output setting Voltage 1 300V Current 1 400mA Timer 1 999min or OFF Display Operation LED Dial button type Detection AE 1360 EzStainSilver AE 1340 EzStainAQua Silver staining kit CBB staining Silver staining kit for detection in a short time approx 1 hour with simple preparation The detected protein can be used for MS CBB staining without acetic acid and methanol Ready to use no preparation required High sensitivity several ng detection is possible in a short time High Performance Power Supply WSE 3100 PowerStation Ghibli I Touch panel type high performance power supply Voltage 0 500V 3 500V setting Current 0 3000mA 10 3000mA setting Power 0 200W 1 200W setting Timer 1 999min or OFF Display Operation Large color LCD Touch panel type Power supply WSE 3200 PowerStation General purpose power supply Voltage 0 1000V 10 1000V setting Current 0 500mA 10 500mA setting Power 0 200W 1 200W setting Timer 1 999min or OFF Display Operation LED Dial button type 10

e PAGELR Mini size Standard type mini size approx 9 8cm pre cast gel E T520L e PAGEL For 18 samples 14 samples Features A ordable price Various gel types Gel concentration sample quantity A wide range of application For SDS PAGE A wide range of molecular weight separation the second dimension electrophoresis Native PAGE DNA PAGE etc Improved gel quality Sharp bands Smiling less patterns Please visit our website to check the videos Operation overview Set the power supply Connect the AC code Set the gel to electrophoresis chamber Open the package of e PAGEL e PAGEL pre cast gel and PageRunAce 11 Start electrophoresis Fill with electrode bu er Running time High mode approx 25min only for EHR ePAGEL Stanndard mode approx 70min Compatible apparatus Mini PAGE system with a built in power supply WSE 1150 PageRunAce Load samples High resolution mini PAGE system AE 6530P mPAGE Mini slab size electrophoresis apparatus for polyacrylamide gel WSE 1165 Mini slab

Mini size Specifications E T e PAGEL Model Name Code Gel size E R e PAGEL 90mm W 83mm L Plate size Sample wells 120mm W 100mm L 14 wells 4 2mm thickness maximum loading volume 24 L Expiry date Package 18 wells 2 9mm thickness maximum loading volume18 L 7 5 10 12 5 15 consistent Model Principal materials total 5mm thickness 5 20 10 20 gradient polyacrylamide gel Gel concentration The molecular weight range E D e PAGEL 1mm thickness Code Number of wells samples E T7 5L 2331800 14 E R7 5L 2331700 18 E D7 5L 2331940 None E T10L 2331810 14 E R10L 2331710 18 E D10L 2331910 None E T12 5L 2331820 14 E R12 5L 2331720 18 E D12 5L 2331920 None E T15L 2331850 14 E R15L 2331750 18 E T520L 2331830 14 E R520L 2331730 18 E D520L 2331930 None E T1020L 2331840 14 E R1020L 2331740 18 polyacrylamide gel None 5 20 7 5 10 12 5 The molecular weight range Gel concentration Protein Nucleic acid 7 5 40 400 kDa 200 3000 bp 10 20 300 kDa 100 2000 bp 12 5 10 250 kDa 70 1800 bp 15 2 200 kDa 50 1500 bp 5 20 5 400 kDa 30 2500 bp 10 20 2 300 kDa 30 2000 bp Gel polyacrylamide Plate glass Comb PP mold 6 months when stored in a refrigerator NOT to be frozen 10 plates per box Please select the apparatus for pre cast gel PAGEL electrophoresis Refrigeration at 5 10 is recommended for storage If stored in a cold air outlet or below 4 the gel will freeze and become unusable Can be transported in room temperature short time The molecular weight range of protein separation is based on electrophoresis when using EzRun 25mM Tris 192mM glycine 0 1 SDS The molecular weight range of DNA separation is based on electrophoresis when using EzRunTG 25mM Tris 192mM glycine The migration varies depending on the component of the electrode bu er EzRunMOPS electrophoresis bu er is recommended for high speed electrophoresis p PAGEL is for low molecular weight PAGEL NPU 5L 5 gel concentration and NPG 310L 3 10 gel concentration are for high molecular weight 12

e PAGELR Mini size Electrophoresis patterns E T7 5L e PAGEL SDS PAGE Gel E T7 5L 7 5 14wells gel Sample AE 1440 EzStandard molecular weight marker etc Electrode bu er AE 1410 EzRun 25mM Tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6531 PageRun Power Constant current 20mA 80min Staining AE 1340 EzStainAQua CBB staining Molecular weight Da 200 000 97 200 66 400 45 000 E T10L e PAGEL SDS PAGE Gel E T10L 10 14wells gel Sample AE 1440 EzStandard molecular weight marker etc Electrode bu er AE 1410 EzRun 25mM Tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6531 PageRun Power Constant current 20mA 80min Staining AE 1340 EzStainAQua CBB staining Molecular weight Da 200 000 97 200 66 400 45 000 29 000 20 100 E T12 5L e PAGEL SDS PAGE Gel E T12 5L 12 5 14wells gel Sample AE 1440 EzStandard molecular weight marker etc Electrode bu er AE 1410 EzRun 25mM Tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6531 PageRun Power Constant current 20mA 80min Staining AE 1340 EzStainAQua CBB staining Molecular weight Da 200 000 97 200 66 400 45 000 29 000 20 100 14 300 E T15L e PAGEL SDS PAGE Gel E T15L 15 14wells gel Sample AE 1440 EzStandard molecular weight marker etc Electrode bu er AE 1410 EzRun 25mM Tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6531 PageRun Power Constant current 20mA 80min Staining AE 1340 EzStainAQua CBB staining 13 Molecular weight Da 200 000 97 200 66 400 45 000 29 000 20 100 14 300

Mini size E T520L e PAGEL SDS PAGE Molecular weight Da Gel E T520L 5 20 14wells gel Sample AE 1440 EzStandard molecular weight marker etc Electrode bu er AE 1410 EzRun 25mM Tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6531 PageRun Power Constant current 20mA 80min Staining AE 1340 EzStainAQua CBB staining 200 000 97 200 66 400 45 000 45 000 29 000 20 100 14 300 E T1020L e PAGEL SDS PAGE Molecular weight Da Gel E T1020L 10 20 14wells gel Sample AE 1440 EzStandard molecular weight marker etc Electrode bu er AE 1410 EzRun 25mM Tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6531 PageRun Power Constant current 20mA 80min Staining AE 1340 EzStainAQua CBB staining 200 000 97 200 66 400 45 000 29 000 20 100 14 300 E D520L e PAGEL 2D Molecular weight Da Gel E D520L 520 Gel for 2D Sample AE 1440 EzStandard molecular weight marker etc Electrode bu er AE 1410 EzRun 25nM Tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6531 PageRun Power Constant current 20mA 90min Staining AE 1340 EzStainAQua CBB staining 200 000 97 200 66 400 45 000 29 000 20 100 14 300 Comparative patterns of electrophoresis buffer gel gel gel gel M o l e c u l a r w e i g h t ra n g e r e l a t i v e migration distance di ers depending on the kind of electrode bu er EzRunMOPS enables separation on the lower molecular weight side has a wider molecular weight area than EzRun with Tris Glycine SDS In other words EzRunMOPS can provide gradient gel like electrophoresis patterns even on xed concentration gels 14

e PAGELR Mini size E T7 5L e PAGEL DNA Gel E T7 5L 7 5 14wells gel Sample Molecular weight marker etc Electrode bu er WSE 7055 EzRunTG 25mM Tris 192mM glycine Electrophoresis apparatus AE 6531 PageRun Power Constant current 20mA 80min Staining Ethidium bromide staining Molecular weight bp 1500 1000 900 800 700 600 500 400 300 200 100 E T10L e PAGEL DNA Gel E T10L 10 14wells gel Sample Molecular weight marker etc Electrode bu er WSE 7055 EzRunTG 25mM Tris 192mM glycine Electrophoresis apparatus AE 6531 PageRun Power Constant current 20mA 80min Staining Ethidium bromide staining E T10L e PAGEL DNA Gel E T12 5L 12 5 14wells gel Sample Molecular weight marker etc Electrode bu er WSE 7055 EzRunTG 25mM Tris 192mM glycine Electrophoresis apparatus AE 6531 PageRun Power Constant current 20mA 80min Staining Ethidium bromide staining 15 1500 1000 900 800 700 600 500 400 300 200 100 DNA Gel E T10L 10 14wells gel Sample Molecular weight marker etc Electrode bu er WSE 7055 EzRunTG 25mM Tris 192mM glycine Electrophoresis apparatus AE 6531 PageRun Power Constant current 20mA 80min Staining AE 1360 EzStain silver silver staining E T12 5L e PAGEL Molecular weight bp Molecular weight bp 1500 1000 900 800 700 600 500 400 300 200 100 Molecular weight bp 1500 1000 800 600 400 300 200 100 900 700 500

Mini size E T15L e PAGEL DNA Molecular weight bp Gel E T15L 15 14wells gel Sample Molecular weight marker etc Electrode bu er WSE 7055 EzRunTG 25mM Tris 192mM glycine Electrophoresis apparatus AE 6531 PageRun Power Constant current 20mA 80min Staining Ethidium bromide staining E T520L e PAGEL 1500 1000 500 400 300 200 100 DNA Gel E T520L 5 20 14wells gel Sample Molecular weight marker etc Electrode bu er WSE 7055 EzRunTG 25mM Tris 192mM glycine Electrophoresis apparatus AE 6531 PageRun Power Constant current 20mA 80min Staining Ethidium bromide staining E T1020L e PAGEL Molecular weight bp 1500 1000 900 800 700 600 500 400 300 200 100 DNA Molecular weight bp Gel E T1020L 10 20 14wells gel Sample Molecular weight marker etc Electrode bu er WSE 7055 EzRunTG 25mM Tris 192mM glycine Electrophoresis apparatus AE 6531 PageRun Power Constant current 20mA 80min Staining Ethidium bromide staining Related products 1500 1000 500 400 300 200 100 Please refer to the end of this booklet for more information of ATTO Ez Series reagents Loading bu er WSE 7040 EzApplyDNA Sample loading solution for DNA electrophoresis High visibility of red and blue stain Sharp DNA bands Detection AE 1360 EzStainSilver Siver staining solution Short time detection about 1 hour with simple preparation More sensitive detection than uorescent staining Electrode bu er WSE 7055 EzRun TG Tris glycine bu er for electrophoresis Dilute 10x in DW Use this electrode bu er for DNA electrophoresis of PAGEL WSE 7130 EzFluoroStainDNA Fluorescent staining solution Safer and more sensitive than EtBr ethidium bromide Safety Low value in Ames test No carcinogenic substances classi ed by IARC High sensitivity Safer and more sensitive detection than UV by using BluLED as excitation light 16

Mini size p PAGELR Mini size approx 9 8cm pre cast gel Separation of low molecular weight proteins and polypeptides into sharp bands in a short time Electrophoresis time in a minimum of 60min Features For low molecular weight protein A wide range of molecular weight separation Corresponding to wide range of molecular weight 1000Da 75000Da Sharp bands Detection of low molecular weight regions with sharp bands Shortened electrophoresis time Electrophoresis can be completed in about 60 min Even shorter than traditional Tris tricine PAGE Extended expiry date Can be stored in refrigerator 5 10 C for 1 year 2 types of sample well quantity For 14 samples 18 samples same as e PAGEL Compatible apparatus Since it is difficult for sufficient separation by SDS PAGE Laemmli method electrophoresis with Tris tricine based buffer is used for electrophoresis of proteins and polypeptides with low molecular weight less than about 20 000 Da The original method is Shagger s method but it takes more than 10 hours for electrophoresis requires a lot of time for gel preparation and requires different buffers for anode and cathode electrode however this system can solve all of the above issues Operation overview Same as e PAGEL HR and e PAGEL compatible apparatus and operation 17 The gel p PAGEL and electrode buffer EzRunT are specially for the Tris tricine system for low molecules The minimum running time is about 60 minutes which is shorter than conventional methods For detection staining the samples must be fixed immediately after electrophoresis or detected in a short time otherwise they will diffuse Quick staining and de staining are possible by using microwave when CBB staining For blotting semi dry method 0 2 m PVDF membrane Clearblot P membrane is used and methanol is added to the blotting solution to prevent coming o from the membrane Normal CBB staining

Mini size low molecular weight Specifications P T16 5S p PAGEL 2332260 Model Name Code Gel size P R16 5S p PAGEL 2332265 90mm W 83mm L Plate size 120mm W 100mm L 14 wells 4 5mm thickness maximum loading volume28 L Sample wells Gel concentration 16 5 consistent The molecular weight range 1mm thickness total 5mm thickness 18 wells 2 9mm thickness maximum loading volume18 L polyacrylamide gel 1000 75000 Da cp PAGEL Please refer to the speci cations of p 4 for low molecule separation 10 months when stored in a refrigerator NOT to be frozen of compact size gel size approx 60 x 60 mm 10 plates per box Principal materials Gel polyacrylamide Expiry date Package Plate glass Comb PP mold Please refer to pre cast gel PAGEL speci cation of ATTO Page Run Ace Mini Slab and mPAGE series for electrophoresis apparatus Should be stored at 5 10 in a refrigerator If stored in a cold air outlet or below 4 the gel will freeze and become unavailable Can be transported in room temperature for a short period of time The molecular weight cut o range is based on electrophoresis which is used bu er of EzRunT tris tricine SDS Electrophoresis pattern P T16 5S p PAGEL SDS PAGE Molecular weight Da Gel P T16 5S 16 5 14wells gel Sample molecular weight marker EzStandard etc Electrode bu er AE 1415 EzRun T tris tricine SDS Molecular weight Da Electrophoresis apparatus AE 6530 mPAGE 29 000 Power Constant current 20mA 60min Staining CBB staining 97 200 66 400 45 000 29000 20 100 17 000 14 300 14 300 6 500 3 500 1 000 Related products AE 1415 EzRun T Components tris tricine SDS Powder Volume Powder for approx 5L Storage at room temperature for 1 year WSE 7025 EzStandard LMW Da Components 6 kinds of proteins and polypeptides 1000 3500 6500Da 14 3 17 29kDa Volume 2 5 L well for 500 1000 times 17000 Storage year in freezer 6 months in refrigerator after preparation 14300 29000 Easy to prepare just dissolve in distilled water 6500 Sharp bands As a powder it does not take much space long term storage is possible 3500 Long term stability 1000 Reasonable price Easy and quick preparation in combination Low molecular weight with pre cast gels for low molecular weight Low molecular weight marker electrode bu er proteins EzStandard LMW EzRunT 18

PAGELR Mini size Mini size approx 9 8cm pre cast gel Features Compatible apparatus Reliable quality Available for high molecular weight Gel concentration sample quantity A wide range of application Operation overview Same as e PAGEL HR and e PAGEL compatible apparatus and operation For SDS PAGE Native PAGE DNA PAGE etc Specifications Model Name Code NPU 5L PAGEL NPG 310L PAGEL Gel size 90mm W 83mm L Plate size 120mm W 100mm L 5 consistent Model The molecular weight range Principal materials Expiry date Package 1mm thickness total 5mm thickness 12 wells 4 5mm width maximum loading volume 28 L Sample wells Gel concentration NPG SPG 520L PAGEL polyacrylamide gel Code 3 10 gradient gel Number of wells Gel concentration 5 20 gradient gel The range of molecular weight fractionation Protein Nucleic acid NPU 5L 2331192 12 5 50 600 kDa 250 4000 bp NPG 310L 2331194 12 3 10 20 600 kDa 50 4000 bp NPG 520L 2331150 12 SPG 520L 2331120 12 5 20 10 400 kDa 20 2000 bp samples Gel polyacrylamide Plate glass Comb PP mold 6 months when stored in a refrigerator NOT to be frozen 10 plates per box Please use the apparatus for pre cast gel PAGEL electrophoresis Refrigeration at 5 10 is recommended for storage If stored in a cold air outlet or below 4 the gel will freeze and become unusable Can be transported in room temperature short time The molecular weight range of protein separation is based on electrophoresis when using EzRun 25mM Tris 192mM glycine 0 1 SDS The molecular weight range of DNA separation is based on electrophoresis when using EzRunTG 25mM Tris 192mM glycine 19

Mini size Electrophoresis pattern NPU 5L PAGEL SDS PAGE Gel NPU 5L 5 12wells gel Sample Molecular weight marker etc Electrode bu er AE 1410 EzRun 25mM Tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6530 mPAGE Power Constant current20mA 80min Staining CBB staining Molecular weight Da 227 000 116 000 97 200 66 400 55 600 NPG 310L PAGEL SDS PAGE Gel NPG 310L 3 10 12wells gel Sample Molecular weight marker etc Electrode bu er AE 1410 EzRun 25mM Tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6530 mPAGE Power Constant current 20mA 80min Staining CBB staining Molecular weight Da 227 000 116 000 97 200 66 400 55 600 45 000 35 700 29 000 20 100 SPG 520L PAGEL SDS PAGE Gel SPG 520L 5 20 12wells gel Sample Molecular weight marker etc Electrode bu er AE 1410 EzRun 25mM Tris 192mM glycine 0 1 SDS Electrophoresis apparatus AE 6530 mPAGE Power Constant current 20mA 80min Staining CBB staining Molecular weight Da 225 000 150 000 100 000 97 200 75 000 66 400 50 000 45 000 29 000 20 100 14 300 NPG 520L PAGEL Native PAGE Gel NPG 520L 5 20 12wells gel Sample Protein Standard substance Electrode bu er WSE 7055 EzRun TG 25mM Tris 192mM glycine Electrophoresis apparatus AE 6530 mPAGE Power Constant current 20mA 80min Staining CBB staining 20

m PAGEL R Wide size High performance wide size approx 14 8cm pre cast gel Capable of electrophoresis up to 60 samples 2 gels in about 35 min Electrophoresis time in a minimum of 35min 30 samples Features 30 samples per gel is available Corresponding to multi pipette spacing High quality performance of gel High speed electrophoresis is possible Migration is completed in a minimum of 35 min Clear separation sharp electrophoresis patterns Smiling less patterns Expiry date 1 year High cost performance A wide range of application For SDS PAGE Native PAGE DNA PAGE etc Operation overview P re par e and o pe n t h e package of m PAGEL Fill with electrode bu er Set the gel to electrophoresis chamber Load samples Start electrophoresis Running time 21 approx 35min

Wide gel multi sample Specifications M 520L m PAGEL Model Name Code Gel size 2332240 140mm W 80mm L Plate size 160mm W 100mm L 1mm thickness total 5mm thickness 30 wells 2 9mm width maximum loading volume 15 L Sample wells Gel concentration 5 20 gradient polyacrylamide gel The molecular weight range Protein 10 400kDa Principal materials Gel polyacrylamide Nucleic acid 20 2000bp Plate glass Comb PP mold 1 year when stored in a refrigerator NOT to be frozen Expiry date Package 6 plates per box Please use ATTO multiple lane gel electrophoresis system as electrophoresis apparatus Refrigeration at 5 10 is recommended for storage If stored in a cold air outlet or below 4 the gel will freeze and become unusable Can be transported in room temperature short time The molecular weight range of protein separation is based on electrophoresis when using EzRun 25mM Tris 192mM glycine 0 1 SDS The molecular weight range of DNA separation is based on electrophoresis when using EzRunTG 25mM Tris 192mM glycine Data Compatible apparatus High speed electrophoresis SDS PAGE M W 245 000 75 000 25 000 10 000 Electrophoresis of 30 samples gel at once Electrophoresis apparatus WSE 1170 Multiple Lane Gel Electrophoresis system High speed electrophoresis approx 35min for 1 gel Sample Molecular weight marker AE 1440 EzStandard WSE 7020 EzProtein Ladder Gel m PAGEL 5 20 polyacrylamide gel pre cast gel 140 80mm Electrode bu er AE 1440 EzRun Tris Gly SDS CBB staining AE 1340 EzStain AQua CBB staining WSE 1170 Multiple Lane Gel Electrophoresis chamber etc gels max can be run at once Also available with 1 gel Bu er volume 900mL max Electrophoresis of DNA Native PAGE are available 22

Related products Reagents ATTO Ez series ATTO Ez series Reagents We support protein electrophoresis detection and gelmembrane blotting western blotting to detection after electrophoresis For more details of other reagents and products please visit our website or see catalogs Discription Model Product name Code No Protein extraction kit WSE 7420 EzRIPA Lysise kit 2332336 Escherichia coli yeast Protein extraction kit WSE 7423 EzBactYeastCrusher 2332339 Organelle extraction kit WSE 7421 EzSubcell Extract 2332337 Organelle fractionation kit WSE 7422 EzSubcell Fraction 2332338 Phosphate buffered saline WSE 7430 EzPBS 2332380 Sample preparation buffer for SDS polyacrylamide gel electrophoresis AE 1430 EzApply 2332330 Sample preparation kit for 2 D electrophoresis AE 1435 EzApply 2D Kit 2332335 Fluorescent protein labeling kit for SDS PAGE WSE 7010 EzLabelFluoroNeo 2332333 Staining solution of electrophoresis sample WSE 7040 EzApply DNA 2332394 Electrophoresis buffer for SDS PAGE Powder AE 1410 EzRun 2332310 Electrophoresis buffer for SDS PAGE Solution AE 1411 EzRun 2332311 Buffer for SDS PAGE of proteins with high resolution AE 1412 AE 1412S EzRun C 2332320 2332321 Tris tricine electrophoresis buffer AE 1415 EzRun T 2332325 Tris glycine buffer WSE 7055 EzRun TG 2332323 MOPS electrophoresis buffer WSE 7065 WSE 7065L EzRun MOPS 2332326 2332324 Molecular weight marker for SDS PAGE AE 1440 EzStandard 2332340 Polypeptide molecular weight marker for SDS PAGE WSE 7025 EzStandard LMW 2332348 Blue molecular weight marker for SDS PAGE AE 1450 EzStandard PrestainBlue 2332347 Three color prestained marker for SDS PAGE WSE 7020 EzProteinLadder 2332346 staining solution AE 1340 AE 1340L EzStain AQua 2332370 2332371 Silver gel staining solution kit AE 1360 EzStain Silver 2332360 Reverse staining kit AE 1310 EzStain Reverse 2332350 Spin column kit for extraction and recovery of proteins and nucleic acids AB 1171 ATTOPREP MF 3521370 Fluorescent staining kit for detection of DNA WSE 7130 EzFluoroStainDNA 2332395 2021 1 29