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Institute of Animal TechnologyBulletinInstitute of Animal TechnologyVol 57 No 6 June 2021• Congress 2021 Report•AGM• IAT Education on mySociety• AS-ET News•Branch News•Diary Dates

Agenda’s 2020 #TopTech is... Dave Moore!03456 44 55 45 | 01964 671 791hello@agendalifesciences.co.ukwww.agendalifesciences.comIf you want to be our next #TopTech - get in touch today!Find us here on social media →03456 44 55 45 | 01964 671 791hello@agendalifesciences.co.ukwww.agendalifesciences.comEach year Technicians are recommended for Agenda’s #TopTech award for their attitude towards work, their achievements within the industry and their overall support to research.This year we are proud to announce the winner is Dave Moore, who currently works for a client in Oxford. Dave has worked for us for a number of years and has proven time and time again to be an excellent technician for our clients.In 2020, Covid hit the industry hard and Agenda were asked by our clients to provide stafng in different ways. One area we were asked to help was in Covid testing labs. I’m pleased to say we were able to put together a unique team to service one of our clients so they could continue to do Covid testing and expand their capacity.Dave was instrumental in ensuring the team’s tasks were completed on a daily basis, and throughout this difcult time he went above and beyond to ensure our clients were happy.Alongside this, Dave also worked in a number of animal care roles in 2020 in which he showed exceptional animal welfare and demonstrated a professional and dedicated approach to his work. We all celebrate Dave’s achievement of this award and congratulate him for his hard work and support to his peers with a ‘Technician of the Year’ trophy, £100 in vouchers and an extra day off work!Well done Dave, keep up the good work!

EDITOR*Carole Wilsonbulletineditor@iat.org.ukASSISTANT EDITOR*Paul Barwoodbulletinassistant@iat.org.ukADVERTISEMENTMANAGERSPRC Associates Ltdmail@prcassoc.co.ukPublished monthly by theInstitute of Animal Technology*Registered Office:5 South ParadeSummertownOxfordOX2 7JLISSN 0263-2861For enquiries other thanBulletin related contact:IAT ADMINISTRATOR*admin@iat.org.ukor 0800 085 4380BulletinVol 56 No 7 July 2020Final copy date forAugust Bulletin23rd JuneThe opinions expressed in theBulletin do not necessarily reflectthose of the Editor or theInstitute.CONTENTSDear Readers 5___________________________________CLAST information 6-7___________________________________Welcome to MySociety – 8-11The IAT MembershipDatabase___________________________________BING and Branch Reps – 14-15first joint meeting___________________________________AS-ET News 16-18___________________________________Diary Dates 19___________________________________Jul20:IATB NEW 10/7/20 10:01 Page 3Vol 57 No 6 June 2021CONTENTSFinal copy date for August Bulletin23rd JuneDear Readers 5Minutes of the 35th Annual 7-11General Meeting of the Institute of Animal TechnologyCongress 2021 report 15-24IAT Education on mySociety 28-31AS-ET News 32-33Branch News 35Dispatches... UFAW announces 37virtual conference on recent advances in Animal Welfare Science Diary Dates 39

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BulletinJune 2021 • 5BulletinJuly 2020• 5Dear ReadersKeep Reading!CaroleKeep Reading!Keep Reading!Keep Reading!Keep Reading!Keep Reading!Keep Reading!Keep Reading!Keep Reading!Poverty IAT LAUNCHES EQUALITY AND DIVERSITY GenderRaceSexual OrientationDiscriminationAgeReligion or BeliefDisabilityEthnicityFairAnti-oppres sive practiceFairYouth WorkSexValuesEmpowermentLegislationDifferenc eSocietyEqualRightsStatusOpportunitiesClassBeliefsEducationNOBODY IS THE SAME BUT EVERYONE IS EQUALInstitute of Animal TechnologyAdvancing and promoting excellence in the care and welfare of animals in scienceEnsuring policies, procedures and processes don’t discriminateEducation - equal access to opportunitiesCreating an inclusive culture for allwww.iat.org.uk/equalityGROUPJul20:IATB NEW 30/9/20 12:41 Page 5BulletinJuly 2020• 5Dear ReadersTwo things to tell you, as we graduallycome out of hibernation. The first thing to say,we are now back in printed form, so I do hope youare now returning back to a more structured way ofworking and reading this in the tea room! The second thing, something you will have alreadynoticed – the July edition of the Bulletin is muchsmaller than usual. There are a couple of reasons forthis, obviously while we have been in lockdown, wehave not been attending any conferences, meetings, seminars orworkshops in person, these have all been postponed or pushed intovirtual meetings. Most of which have been done at very short notice – socurrently there is nothing much to report. Additionally, because of theunscheduled changes to our lives, the Bulletin has begun to get verybehind in our print schedule, which we now need to get back as wereturn to printed format. Our August edition will also be small – but Iguarantee our September edition will be back to normal!Back with this edition, we do have some articles of interest to you – let’sstart with MySociety – the IAT membership database. Read pages 8-11 foran explanation of how this works and how you can use it. This is ofinterest to us all, but especially so to those of us who are RAnTechs – wecan use the database to track and keep records of our CPD.Elsewhere, we have AS-ET news, which includes information on the essaycompetition – prize a trip to Congress 2021. See page 16 to see if you areeligible – even if you are not, please pass the information on to thosewho are.Make sure you take care and …Keep Reading!CarolePoverty IAT LAUNCHES EQUALITY AND DIVERSITY GenderRaceSexual OrientationDiscriminationAgeReligion or BeliefDisabilityEthnicityFairAnti-oppres sive practiceFairYouth WorkSexValuesEmpowermentLegislationDifferenc eSocietyEqualRightsStatusOpportunitiesClassBeliefsEducationNOBODY IS THE SAME BUT EVERYONE IS EQUALInstitute of Animal TechnologyAdvancing and promoting excellence in the care and welfare of animals in scienceEnsuring policies, procedures and processes don’t discriminateEducation - equal access to opportunitiesCreating an inclusive culture for allwww.iat.org.uk/equalityGROUPJul20:IATB NEW 10/7/20 10:01 Page 5BulletinJuly 2020• 5Dear ReadersTwo things to tell you, as we graduallycome out of hibernation. The first thing to say,we are now back in printed form, so I do hope youare now returning back to a more structured way ofworking and reading this in the tea room! The second thing, something you will have alreadynoticed – the July edition of the Bulletin is muchsmaller than usual. There are a couple of reasonsfor this, obviously while we have been in lockdown,we have not been attending any conferences, meetings, seminars orworkshops in person, these have all been postponed or pushed intovirtual meetings. Most of which have been done at very short notice – socurrently there is nothing much to report. Additionally, because of theunscheduled changes to our lives, the Bulletin has begun to get verybehind in our print schedule, which we now need to get back as wereturn to printed format. Our August edition will also be small – but Iguarantee our September edition will be back to normal!Back with this edition, we do have some articles of interest to you – let’sstart with MySociety – the IAT membership database. Read pages 8-11for an explanation of how this works and how you can use it. This is ofinterest to us all, but especially so to those of us who are RAnTechs – wecan use the database to track and keep records of our CPD.Elsewhere, we have AS-ET news, which includes information on the essaycompetition – prize a trip to Congress 2021. See page 16 to see if you areeligible – even if you are not, please pass the information on to thosewho are.Make sure you take care and …Happy Reading!CarolePoverty IAT LAUNCHES EQUALITY AND DIVERSITY GenderRaceSexual OrientationDiscriminationAgeReligion or BeliefDisabilityEthnicityFairAnti-oppres sive practiceFairYouth WorkSexValuesEmpowermentLegislationDifferenc eSocietyEqualRightsStatusOpportunitiesClassBeliefsEducationNOBODY IS THE SAME BUT EVERYONE IS EQUALInstitute of Animal TechnologyAdvancing and promoting excellence in the care and welfare of animals in scienceEnsuring policies, procedures and processes don’t discriminateEducation - equal access to opportunitiesCreating an inclusive culture for allwww.iat.org.uk/equalityGROUPJul20:IATB NEW 13/7/20 08:29 Page 5 Here we are, June signalling the Congress edition of the Bulletin. Of course, it was very different this year, as it was held virtually so unfortunately there is not the opportunity for people to scour the pages for images of themselves! However, it was still a very successful meeting and being held virtually meant people who may not have had the means to travel to a venue could attend. See pages 15 – 24 for the Congress report. As you probably know, the AGM is also ordinarily held within Congress however, for the second year in a row it was held virtually and if you managed to miss it then go to pages 7 – 11 for the highlights.Better news for Congress 2022, we are planning to go live and in person again – see pages 11, 25 and 31 for a little taster of what is being planned and dates. Whether it be in person, or virtual, Congress is a great way to share ideas with others, so start thinking now what you could present, what might make a great poster or workshop. I have been really lucky to have been able to attend Congress for many years and over that time I have seen so much inspirational work presented in all forms that has helped increase and improve the welfare of the animals in our charge. So, get thinking about how you can present your work to colleagues that shows how you have made a difference.If you have already made a difference, why don’t you think of applying for The Times Technician of Year Award? There is information on the Diary Dates, as it is a prestigious award that John Waters won last year. Until next month

Bulletin6 • June 2021 Manufactured in the UKFREE TRIAL samples

BulletinJune 2021 • 7Manufactured in the UKFREE TRIAL samplesMinutes of the 35th Annual General Meeting of the Institute of Animal Technology 14.00 hours on Tuesday 9th March 2021, via ZoomThe AGM was chaired by the IAT President, Robin Lovell-Badge FRS and was attended by 91 members. Linda Horan (LH) welcomed everyone to the meeting, especially the overseas visitors who were able to attend.Robin Lovell-Badge (RLB) then welcomed everyone to the 35th IAT AGM.1. Apologies Apologies had been received from Adele Kitching.2. Minutes of the 34th Annual General Meeting of the Institute of Animal Technology held on 8th July 2020 via Zoom The minutes were unanimously accepted as a true and accurate record of the meeting, with 65 votes for, none against and 4 abstentions. 3. Matters arising from the minutes Simon Cummings (SAC) reported that the minutes had been published in full on the website and an abridged version in the Bulletin. Item 10.2 Following on from being appointed an End Point Assessor for Level 2 Apprenticeships, the IAT are using video conferencing for assessments. RAnTech interviews are also currently being carried out via Zoom.4. Correspondence There was nothing to be covered under this item.5. Annual Report The full IAT Annual Report had been published on the IAT website prior to the AGM and all members notiﬁ ed via email. Hon Secretary SAC spoke to the Annual Report, thanking IAT Council for all their hard work during a challenging year and highlighted various areas of the report.

Bulletin8 • June 2021 SAC outlined the main objectives for Council for 2021:1. Continue to update the IAT Education syllabi for Levels 4, 5 and 6.2. Strengthen links with external partners.3. Grow IAT Education proﬁle and apprenticeships.4. Continue to support the membership and be visible in the current COVID-19 pandemic.5. Increase proﬁle of Equality and Diversity Group and undertake membership survey. The full Annual Report is available on the members section of the IAT website. There were no questions on the annual report.6. Financial Report Glyn Fisher (GF) spoke to the accounts for 2020. These accounts are available on the IAT website. GF reported a surplus of expenditure over income of £57,346. The main reason for this operating loss is a reduction of income from nearly all areas of the Institute, due to the COVID-19 outbreak. AST2020 was declared non-viable on grounds of frustration, there has been a reduction in educational income and a reduction in advertising across all publications. This has been the same across the whole industry. The IAT has, however, carried on operating. Going forward savings will need to be made and some areas have been highlighted for this. There will be reduced running costs for IAT Council meetings, with three out of ﬁve meetings being held virtually from 2022. It is likely that only one face to face meeting will take place in 2021. Other meetings, such as the Registration & Accreditation Board and Board of Educational Policy will also be virtual. The ATW has moved to wholly electronic publication which has reduced printing and postage costs. There will be a general ‘tightening of belts’ across all areas. GF reported that there will be no changes to membership subscriptions for 2022. All other fees will also remain unchanged for 2022. All prices are displayed on the IAT website. The Financial Report and the Audited Accounts were then proposed. Proposed: Glyn Fisher Seconded: Steven Cubitt Carried by a majority of 64 votes for, 1 against and 4 abstentions.

BulletinJune 2021 • 9 7. Appointment of the Auditor The Treasurer proposed that King Loose be re-appointed as auditors for 2021. They have done a sterling job in 2020 and have been a great asset to the Institute. Proposed: Glyn Fisher Seconded: Jenny Parks Carried by a majority of 57 votes for, none against and 6 abstentions.8. Election of Ofﬁcers Linda Horan, Chair IAT Council, took over as chair of the AGM for the appointment of the President.8i. President Council wished to propose Robin Lovell-Badge CBE FRS as President for the forthcoming year. Proposed: Council Seconded: Michael Barnes Carried by a majority of 68 votes for, none against and 2 abstentions. LH was delighted to welcome RLB back as President of the Institute.8ii. Vice-Presidents RLB reported that the last President’s Advisory Group meeting (held via Zoom) had been very well attended and a further meeting would be held shortly. No new Vice Presidents were proposed. The following were proposed en bloc as Vice Presidents: David Anderson Stephen Barnett Miles Carroll Paul Flecknell Penny Hawkins Wendy Jarrett Judy MacArthur Clark Fiona McEwen Tim Morris Clive Page Jan-Bas Prins Vicky Robinson Gail Thompson Robert Weichbrod Senga Allen Paul Sanders David Spillane Proposed: Council Seconded: Robin Labesse Carried by a majority of 59 votes for, none against and 4 abstentions.

Bulletin10 • June 2021 8iii. Council There were eleven candidates for eleven vacancies on Council, therefore no election was held and the following eleven candidates were appointed to serve a three-year term: Carmen Abela Nicky Gent Diane Hazlehurst Sam Jameson Robin Labesse Theresa Langford Alan Palmer Allan Thornhill John Waters Carole Wilson Adrian Woodhouse Congratulations to all those joining and re-joining Council. 9. Proposals for Honorary and Life Membership There were no proposals for Life or Honorary Members.10. Any Other Business10.1 RLB highlighted that there had been a high emotional toll on IAT members, particularly at the beginning of the year, with anecdotal reports of mass culls of animals. How much is the IAT monitoring the emotional welfare of members? LH reported that the Equality and Diversity survey will address this. It was originally due to be sent out after the ﬁrst lockdown, but then further lockdowns occurred, members were too busy to respond and often were split into working teams which did not reﬂect normal working practices. The Equality and Diversity Group introduced a ‘Coffee Catch Up‘ open meeting on Wednesday afternoons, for people to drop into. It was suspended for the three weeks of Congress but will pick up again after that.10.2 A vote of thanks to the Congress Committee was proposed, for all their efforts in putting together a virtual Congress. LH seconded this and stated that the whole of Council had been exceptional during the pandemic.10.3 Another vote of thanks was given to all those working behind the scenes. RLB added his thanks to all of Council for their hard work, but also to those who have contributed during the year. He also thanked everyone for attending the AGM.

BulletinJune 2021 • 1110.4John Waters wished to thank Haley Daniels for all her hard work on the Diversity and Equality Group and her support of animal technicians via this group.10.5Gail Thompson thanked the Congress Committee for making it so easy for her to attend the AGM. There being no other business, RLB thanked IAT Council for all their hard work over the year. RLB also thanked AT for running the Zoom webinar. The meeting was closed at 14.40 hours.Animal Technologists – the key workers for medical researchCALL FOR PAPERSl take an active part in the leading annual meeting for Animal Technologistsl present a paper and qualify for free attendance at Congressl make this your debut presentation year – ﬁrst time presenter papers are only 20 minutes long and as well as a free congress there is a prize for the one judged to be the bestl send your ideas today on the Submission form available from www.iat.org.ukl ﬁnal date for submissions: Friday 29th October 2021Contact: congress@iat.org.ukCongress2022CONGRESS Invitation to Participate29th March – 1st April

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BulletinJune 2021 • 15SOMNI RODENTNRB SYSTEMSyringe tube ≠ nose conesNo more tapeSEALEDCOMPATIBLE WITH EXISTING UK/EU:ProductsAccessoriesFittingsSOMNI Scientific is centered on the animal welfare and research community with a focus on clinical accuracy, clinician/technician safety, economic performance and intuitive functionality.SOMNI PROVIDES UNPARALLELED CUSTOMER SERVICE, CLINICAL AND TECHNICAL SUPPORT.Following a year of lockdowns and cancelled meetings, it was agreed that the only option for Congress 2021 was to hold a virtual event. None of the committee had organised one of these virtual meetings before (who had?!) but luckily they knew a man who could ﬁ nd out!The committee had already decided to use the Aventri system in order to faciliatate registration, website, onsite check in and invoicing for our events and with a few tweaks, we transferred across to their virtual platform.Congress 2021 saw a full range of interactive workshops and presentations, as well as poster displays and interactive input from our trade exhibitors. IntroductionAnimalbehaviourisanobservablemeasureoftheeffectstheexternalenvironmenthasonananimal.Therelativelynon-invasivenatureofbehaviouralanalysisminimisestheinfluenceofhumanpresence.Appropriateacclimatisationtoanovelexperienceorenvironmentis animportantrefinementtechniqueadoptedbyanimalresearchfacilitiestoimprovewelfare.Miguel-Pachecoetal.(2014)1usedCCTVtoinvestigatetheimpact ofcattlelamenessoneatingandmilkingbehaviour.Theyfoundlamecattlefedlessandvolunteeredthemselveslessfrequentlyformilkingcomparedtotheirable-bodiedconspecificssuggestinglamenessimpactsbehaviourandwillingnesstobemilked.Usinghumanobservation,TempleGrandin(2010)2demonstratedhabituatingnaïveantelopeandbisontorestraintcratesandregulatedprocedures(e.g.bloodsampling)improvedhandlingandreducedcortisollevelsastheanimalswereoverallcalmerandlessflighty.Throughhabituation,Grandinwasabletoimprovetheanimal’swelfareduringregulatedproceduresbyreducingtheoverallstresscausedbytherestrainingprocess.ThePirbrightInstitute(TPI)hasbeentriallingtheuseofbehaviouralanalysisthroughCCTVusageandhumanobservationtomeasurecattleacclimatisationtoahigh-containmentSAPO4facility.Phase 1: Observation Objective: To determine the length of time needed for acclimatisationby analysingthe voluntary entry rate of cattle into yokes recorded through human observation.•Duringphaseone,17cattlewereyoked(seefigure2.)inthemorningover12daysaspartofthefeedingroutine,7ofthosewereadditionallyyokedintheafternoon.•Feedbucketswereplacedout,the yokeswereopened,andthestaffmembersthenwaitedoutofsightforthecattletoenter.•Thecattlewereinitiallyallowedsixminutestoentertheyokesbythemselves.Itwasthendecidedthiswastoolongandreducedthetimedowntothreeminutes.•Afterthetimeallowed,iftheyhadnotenteredcoaxingtheminwiththefeedbucketwaspermitted.•The numberofcattleenteringvoluntarily,beingcoaxedorrefusingtoyokewererecordedonthedatacollectionsheet.Acommentsboxwasavailableforanyadditionalinformatione.g.afullhealthcheck,bloodsampling,vaccination.ConclusionsThesepilotstudiesindicatecompleteacclimatisationoccursarounddaysfourtosixyetfurtherstudyisneededtoconfirmthis.Fromthedatagenerated,behaviouralanalysisandCCTVuseappeartobeveryusefulinhelpingdetermineandfurther improvetheappropriateacclimatisationperiodforcattleinahigh-containmentenvironment.Thisisessentialinaidingrefinementtoimprovebothanimalwelfareandscientificintegrity.FutureStudiesDatacollectedfromIceRoboticsmotiontrackingbraceletsandadditionalNolduscollartrackingsoftwarewillhelpexpandthecurrentlocomotiondata.Ongoingstudiesintotheuseofanimal mountedaccelerometersandlocationtrackingsystemsarealsobeingconducted.The presentyokingstudywillalsobeincludingmoreCCTV footageandtimings.Thiswillbeusedtodeterminehowlongittakesthecattletoentertheyokesusingthepremisethatstressedcattlewillenterslowerthanthosethatare fullyhabituated.Ethicalstatement:Alllegalandethicalaspectswereconsideredwhenconductingthisstudy,includingtheuseofanimals.Thoseparticipatinginthisstudyconsentedtotakepartandwereawareofanyimagerecordingthattookplace.Figure 1.The CCTV camera angle used for video footagePhase2:TimeTrialObjective: To determine the length of time needed for acclimatisationby analysing the voluntary entry rate of cattle into yokes recorded through cattle yoking timings.•Duringphasetwo,44cattlewereyoked(seefigure4.)inthemorningover7daysaspart ofthefeedingroutine.•Feedbucketswereplacedout,theyokeswereopened,andthestaffmembersthenwaitedoutofsightforthecattletoenter.•Asaresultofphaseone,thecattlewereallowedthreeminutestoentertheyokesbythemselves.•Afterthetimeallowed,iftheyhadnotenteredcoaxingtheminwiththefeedbucketwaspermitted.•Thenumberofcattleenteringvoluntarily,beingcoaxed orrefusingtoyokewererecordedontheaforementioneddatacollectionsheet.•TheCCTVcameraswerethenconsultedand,usingWaveview,theamountoftimeittookeachanimalfromthreestudygroups(studyoneN=5,studytwoN=2,studythreeN=4)toentertheyokeswasdetermined.Thiswasthenanalysed.Improving welfare of cattle housed in a high-containment facility using behavioural analysis References1.Miguel-Pacheco,G.G.,Kaler,J.,Remnant,J.,Cheyne,L.,Abbott,C.,French,A.P.,Pridmore,T.P.andHuxley,J.N.(2014)Behaviouralchangesindairycowswithlamenessinanautomaticmilkingsystem,AppliedAnimalBehaviourScience,Vol150,pages1-82.Grandin,T.(2010)HabituatingAntelopeandBisontoCooperateWithVeterinaryProcedures,JournalofAppliedAnimalWelfareScience,vol3(3),pages253-261Results•Betweendaysfourandsixthenumberofcattlevoluntarilyyokingplateaued withaminordecreaseseen ondayfive(figure3)whichis yetunexplained.•Adecreaseinvoluntaryyokingwasseenonday threepossiblyasaresultofbeinghealthcheckedandhavingtheirfeetinspectedwhilstintheyokes thepreviousday.Theymayhavenotbeenfullyhabituatedtotheyokesandenvironmentatthispoint.•Nodecreaseinthenumberofcattleenteringtheyokeswasobservedthedayaftertheirfirstregulatedprocedure(dayeight)suggestingfullacclimation.•BeingyokedPMaswellasAMdidnotseemtohaveanoticeablepositiveornegativeeffectonthecattleenteringtheyokesFigure 2 . Cattle in the yokes during feeding timeFigure4.Phase2:Totalnumberofcattle(N=44)observedenteringtheyokesduringtheAMyokeoftheacclimatisationperiodcollatedfromthreestudies(17/02/2020-18/07/2020)Results•Through humanobservation,the cattlevoluntarilyyokingplateausondayfour(seefigure4).Areductioninnumberscanbeseenatdaysixonwardswheretwowereeuthanizedduetothestudyending.•Whenanalysingthetimings,thecattlevoluntarilyself-yokethequickestondaysix,withalargereductionintimeseenbetweendaysfour andsixforbothgroupsoneandtwo(seefigure5).•Theamountoftimereducesfromdayfourandcontinuestodososuggestingaroundthistimetheyaremorecomfortableinanovelenvironment andbeingyoked.00:00:0000:00:1700:00:3500:00:5200:01:0900:01:2600:01:4400:02:0100:02:1800:02:361234567891011Time taken (hh:mm:ss)DayStudy 1Study 2Study 3Figure 5. Phase 2: The average amount of time taken per day for cattle (N=11) to self-yoke across three separate studies (17/02/2020 –18/07/2020)05101520253035404501234567Number of cattleDayVoluntaryCoaxedRefusedLinear (Voluntary)Figure3.Phase1:Yokingfrequenciesof17cattleatTPIduringthecombinedAM (N=17)andPM(N=7)yokeovertheacclimatisationperiodfrom28/10/2019-09/11/2019*Day0 -day of arrival**Day 2 -Feet and health check***Day 7 -Blood sampling and vaccination0246810121416180*12**34567***89101112Number of cattleDayVoluntary AMCoaxed AMVoluntary PMCoaxed PMShining a light on rearing pigmentlesszebrafishJacqueline Glover*, Thom Berriman*, Dimitra Mantzorou*, William Havelange*, Sam Berry* and Bruno Correia da Silva**King’s College London, Zebrafish Facility, Biological Services Unit.2. Materials and MethodsThree strains of zebrafish, varying in their degree of pigmentation, were selected (Wildtype AB, Albinob4and Crystal Mutant (nacrew2/w2;albb4/b4;roya9/a9)). Twelve rows, on the same recirculating system, were filled with empty tanks. A lux meter was used to calculate the average light intensity (LUX) of each row from inside the tanks. Three rows were selected to represent high (259 LUX), medium (128 LUX) and low (38 LUX) light intensity levels (Figure 1).050100150200250WildtypeAlbinoCrystalAverage number of fertile eggs laid per breeding pairZebrafish strainHighMediumLowFigure 5 Average number of fertile eggs laid by three zebrafish strains raised at three different LUX levels. Error bars show standard error. 050100150200250300123456789101112Average light intensity (LUX)Row position on rackFigure 1 Average light intensity (LUX) of tanks across two racks. High, medium and low light intensity used for the purpose of this study are shown in red. 1. IntroductionZebrafish (Danio rerio) strains with mutated pigment cells allow clear in-vivoimaging to be carried out. In 2016, Crystal mutants, which lack most pigment cells in both their body and eyes, were first produced at King’s College London (Antinucciand Hindges, 2016). However, evidence shows strains with mutated pigment cells exhibit reduced survival during the early rearing stages in comparison to wildtype strains (Wilson, 2012; Barwoodet al., 2017). The Crystal strain, in particular, has poor survival in the nursery compared to wildtypes, and anecdotal evidence suggests lower spawning success compared to other strains. Light has been shown to influence the survival of wildtype zebrafish larvae (Villamizar et al., 2014), however research of its effect on other zebrafish strains is limited (Barwoodet al., 2017). Light is also well known to influence spawning behaviour in zebrafish, as their reproductive cycle is photoperiod-dependant, with most spawning at dawn (Tsang et al., 2017).This experiment aimed to look at the impact of light intensity as a way to refine methods of rearing pigmentlesszebrafish and to reducethe number of fish needed to maintain important genetic lines, such as the Crystal line. 0102030405060708090WildtypeAlbinoCrystalAverage percentage of pairs spawned (%)Zebrafish strainHighMediumLowFigure 4 Average spawning success of three zebrafish strains raised at three different LUX levels. Error bars show standard error. Three tanks of each zebrafish strain were then assigned to each of the three rows. At 5dpf, fry were transferred into 3.5 litre tanks (n=25 per tank), containing 1 litre of water. All fish were fed according to methods previously described by Mantzorouet al., (2017). Upon reaching sexual maturity (between 58-84dpf) the survival rate of each tank was calculated. All tanks were then reduced to 17 fish per tank. Remaining fish were then paired to spawn, a total of three times, to measure spawning success. Eggs from each pair were collected in separate petri-dishes and the fertile eggs produced by each pair were counted.3. ResultsOf the groups tested, zebrafish strain was found to significantly affect the survival rate of zebrafish during the nursery period (p<0.01) and the survival rate of each strain was found to be significantly different to each other (p<0.01). In contrast, light level had no significant effect on survival rates in any of the zebrafish strains studied (Figure 3).Spawning success was also significantly affected by the strain of zebrafish (p<0.05), with a significant difference found between wildtype and crystal spawning success (p<0.01). In comparison, light level had no significant effect on spawning success in any of the zebrafish strains studied (Figure 4).There was no significant effect of either zebrafish strain or light level on the fertility of any of the zebrafish studied (Figure 5). 0102030405060708090100WildtypeAlbinoCrystalPercentage of nursery survival (%)Zebrafish strainHighMediumLowFigure 3 Average survival of three zebrafish strains during the nursery period when exposed to three LUX levels. Error bars show standard error. Figure 2 Wildtype AB (left), Albino (centre) and Crystal Mutant (right) zebrafish strains. 6. ReferencesAntinucci, P. and Hindges, R. (2016) A crystal-clear zebrafish for in vivo imaging. Scientific Reports. Vol 6, 1-10.Barwood, P., Dunford, K., Moiche, V. and Wilson, C. (2017) Light intensity and its impact on survival rates of pigmentlesszebrafish (Danio rerio). Institute of Animal Technology Congress, Newport, South Wales, 23 March.Mantzorou, D., Berriman, T., Jani, S., Clarke, R. and Correiada Silva, B. (2017) Rise of the rotifers: Brine shrimp, who needs them? Animal Technology and Welfare. Vol 16, 216-218.Tsang, B., Zahid, H., Ansari, R., Lee, R. C., Partap, A. and Gerlai, R. (2017) Breeding zebrafish: A review of different methods and a discussion on standardization. Zebrafish. Vol 14, 1-13.Villamizar, N., Vera, L. M., Foulkes, N. S. and Sánchez-Vázquez, F. J. (2014) Effect of lighting conditions on zebrafish growth and development. Zebrafish. Vol 11, 173-181.Wilson, C. (2012) Aspects of larval rearing. Institute for Laboratory Animal Research. Vol 53, 169-178.4. DiscussionThe results demonstrate that crystal mutants exhibit significantly lower survival rates (Figure 3) in comparison to albino and normally-pigmented wildtype strains. The current study suggests lower levels of pigmentation to be associated with lower survival in zebrafish, which is in line with previous studies (Wilson, 2012; Barwoodet al., 2017).It can also be seen that the average number of spawning crystal pairs was lower than for either wildtype or albino strains (Figure 4). Wildtype and crystal strains show a trend for increased spawning success at the highest LUX level, whilst albino zebrafish spawned best when housed at the lowest LUX level. The study found no significant difference in the average number of fertile eggs laid per strain, which is in agreement with findings of Antinucciand Hindges(2016). All strains showed a tendency for increased fertility when housed at the highest LUX level (Figure 5). To conclude:•Survival was significantly affected by zebrafish strain, but not light intensity. •Fertility was not significantly affected by strain or light intensity. •Spawning success was not significantly affected by light intensity.•Crystal mutants have significantly lower spawning success compared to wildtypes.Jacqueline Glover2. Materials and MethodsThree strains of zebrafish, varying in their degree of pigmentation, were selected (Wildtype AB, AlbinoTwelve rows, on the same recirculating system, were filled with empty tanks. A lux meter was used to calculate the average light intensity (LUX) of each row from inside the tanks. Three rows were selected to represent high (259 LUX), medium (128 LUX) and low (38 LUX) light intensity levels (Figure 1).2Average light intensity (LUX) of tanks across two racks. High, medium and low light intensity used for the purpose of this study are shown in red. 1. IntroductionZebrafish (Danio reriobe carried out. In 2016, Crystal mutants, which lack most pigment cells in both their body and eyes, were first produced at King’s College London (2016). However, evidence shows strains with mutated pigment cells exhibit reduced survival during the early rearing stages in comparison to wildtype strains (Wilson, 2012; Barwoodet al., compared to wildtypes, and anecdotal evidence suggests lower spawning success compared to other strains. Light has been shown to influence the survival of wildtype zebrafish larvae (Villamizar 2014), however research of its effect on other zebrafish strains is limited (2017). Light is also well known to influence spawning behaviour in zebrafish, as their reproductive cycle is photoperiod2017).This experiment aimed to look at the impact of light intensity as a way to methods of rearing maintain important genetic lines, such as the Crystal line. Three tanks of each zebrafish strain were then assigned to each of the three rows. At 5dpf, fry were transferred into 3.5 litre tanks (n=25 per tank), containing 1 litre of water. All fish were fed according to methods previously described by Upon reaching sexual maturity (between 58calculated. All tanks were then reduced to 17 fish per tank. Remaining fish were then paired to spawn, a total of three times, to measure spawning success. Eggs from each pair were collected in separate petriOf the groups tested, zebrafish strain was found to significantly affect the survival rate of zebrafish during the nursery period (p<0.01) and the survival rate of each strain was found to be significantly different to each other (p<0.01). In contrast, light level had no significant effect on survival rates in any of the zebrafish strains studied (Figure 3).Spawning success was also significantly affected by the strain of zebrafish (p<0.05), with a significant difference found between wildtype and crystal spawning success (p<0.01). In comparison, light level had no significant effect on spawning success in any of the zebrafish strains studied (Figure 4).There was no significant effect of either zebrafish strain or light level on the fertility of any of the zebrafish studied (Figure 5). Wildtype AB (left), Albino (centre) and Crystal Mutant (right) zebrafish strains. humanpresencefrequentlyfornaïvehabituation,GrandinCCTVusebothanimalanimalthecattlepartandTo determine the length of time needed for acclimatisationthe voluntary entry rate of cattle into yokes recorded (seefigureroutine.wereopened,cattletoallowedthreeenteredcoaxingbeingaforementionedand,usingfromthreethreeN=4analysed.voluntarilynumberseuthanizedduevoluntarilyreductionintimetwo(seefourandcontinuesmorecomfortable67Study 2Study 3The average amount of time taken per day for cattle (N=11) yoke across three separate studies (17/02/2020 Fera Science Ltd, NAFIC, Sand Hutton, York, North Yorkshire, YO41 1LZ, UKAUTHORSLorna Cleverley, Michelle Powell and Jackie DunnA baited trap system for the early detecon of mites in laboratory animal faciliesBooklice (Liposcelisspp.), storage mites (e.g. Acarus spp., Tyrophagus spp. and Glycyphagid mites) and stored product beetles(e.g. Tribolium spp., Lathridiidae spp., Sitophilus spp. etc) are all common stored-product pests frequently found in laboratory animal facilities. Although they are commonly found they are considered pseudoparasites of laboratory animal facilities. These pests are not generally considered during quarantine of laboratory animals or targeted as part of routine pest control programmes making detection of the early stages of infestation, when numbers are low, extremely difcult. As a result, infestations go unnoticed until numbers have increased dramatically with undesirable consequences (Table 1).We have developed the rst trap specically designed to attract mites in order to allow an early warning/detection system (Figure 1 & 2). The trap is composed of a plastic plate with a circular chamber, an inner chamber holds the attractant media. The chamber has a screw top which is partially opened to allow entry of mites through small gaps at the sides of the chamber. The attractant is anon-toxic agar based food lure but also provides the high humidity favoured by mites. Figure 2 show mites feeding on the food lure.The BT trap has been successfully used in the food and manufacturing industries. Table 2 lists the mites and insects commonly encountered in a BT trap. We propose that this system could be of benet in animal facilities as it could be used to pinpoint sources of mite/insect infestation.Validation of the BT trap for use in laboratory animal facilitiesHistorically the trap has been used and validated as a non-sterile trap. However, to meet the high levels of biosecurity required in laboratory animal facilities we produced a sterile trap with sterile bait. In order to determine if there was any negative impact regarding attraction of mites to the bate traps we performed a short investigation comparing sterile vs non-sterile bate traps in the laboratory. To conrm our ndings we also tested the traps in an animal facility.Laboratory Sterile vs Non-sterile trap lure trial To provide an enclosed environment, the BT traps were placed in to containers. A total of 15 containers were used. Five containing a sterile and non-sterile trap, ve containing a sterile trap only and ve containing a non-sterile strap only. Approximately 100 mixed life stages of Tyrophagus putrescentiae (a cosmopolitan storage mite) were added to each container at a point furthest away from the traps. The containers were then sealed and left at 20°C 80% RH for four days. After four days the traps and containers were then examined. The number of mites in the traps and in the containers was recorded. Figure 3 and Table 3 shows the percent of mites detected in the sterile, non-sterile traps and containers. • Only small numbers of mites were found outside the traps in the container. In all cases the mites found in the container were dead and therefore all mobile mites had entered the traps• When the two types of trap were placed together in a container in a choice test situation, the non-sterilised trap attracted slightly more mites than the sterilised trap(table 3)• When used on their own, both types of trap attracted all mobile mites equally wellAnimal facility testThe sterile and non-sterile traps were tested in a laboratory animal facility which holds multiple animal species. The traps were placed in three separate feed storage area’s. The results of the trial can be seen in Table 4.The results of both investigations demonstrated that sterilising the traps and bait had no adverse effect on the ability of the baited traps to attract mites. INTRODUCTIONREFERENCESCampbell JF,Arthur FH,Mullen MA. 2004. Insect management in food processing facilities. Adv Food Nutr Res 48:239–295.Cui YB. 2014. When mites attack: domestic mites are not just al-lergens. Parasit Vectors 7:411.Hubert J,Stejska lV,Kubatova A,Munzbergov aZ,Vanova MZ d’arkova E. 2003. Mites as selective fungal carriers in stored grain habitats. Exp Appl Acarol 29:69–87.P Ruoppi, T Koistinen, S Pennanen, 2004 Sensitisation to mites in laboratory animal workers with rhinitis Occup Environ Med 2005;62:612–615. Turner BD. 1994. Liposcelis- (Psocoptera, Liposcelididae), a stored food pest in the UK. Int J Pest Manag40:179–190.Warner CE ,BohaneSJ. 1978. Preliminary study of a diet infested with Acarus siro (L.) on adult food consumption and foetal status in mice. J Stored Prod Res 14:13–17. ui YB. 2014. When mites attack: domestic mites are not just al-lergens. Parasit Vectors 7:411ag40:179–190.SummaryWe have demonstrated that the attractant properties of the bait was not affected by the sterilisation process. The BT trap could be a useful addition in developing an holistic monitoring and control strategy that effectively targets all pests that may inadvertently enter an animal facility. The use of the BT trap would facilitate the detection of mites at a critically early stage allowing appropriate control measure to be put in place limiting the population growth of mites and the potentially adverse effects on research facilities. Future investigations to determine if the BT could be used to trap Radfordia species, Myobia species and Myocoptes species directly from rats and mice by placing the traps in change stations and dirty bedding will be undertaken. We propose that the BT mite trap should be used to enhance animal facility biosecurity, closing a gap that currently exists in animal facility biosecurity control. Table 1. Consquences of storage mite infestation in laboratory animal facilities AcariasisEctoparasite misidenticationTransmission of pathogensFinancial lossesOccupational health riskSome laboratory animals may be susceptible to acariasis, a syndrome described in humans that is caused by mite invasion of various tissues (Cui, 2014; Warner& Bohane, 2014).Storage mites, particularly grain mites, can be confused with Myobia, Myocoptes and Radfordia species leading to additional testing or unnecessary treatment and ultimately unnecessary culling of rodent colonies.Mites may also act as vectors or fomites for the transmission of adventitious agents in laboratory animal facilities (Turner, 1997).The damage caused by mite infestation can lead to direct nancial losses due to the deterioration of food stores and/or to the unnecessary culling of rodent colonies.Growing evidence links mites and their by-products to an increase in human allergy and has been demonstrated as a risk associated with laboratory animal facilities (Ruoppi et al., 2014). Acarus siro is one of several mites that have been identied as a cause of intestinal, pulmonary, and urinary acariasis in humans (CUI, 2014).Table 2. Common species detected by the BT trapInsect speciesMite speciesGnatocerus, Triboliom, Ryzopertha, Lathriidae, Dermestid beetles and larvae, Ahasverus, Anthrenus, Typhaea, Sitophlius, psocids, Ptinus, spiders, earwigs, springtails, ies, Stegobium, Lasioderma, Cryptolestes, Oryzaephilus, moths e.g Ephestia.Acarus sp., Glycyphagid sp.; Caloglyphus sp., Sancassania, Tarsonemids, Oribatid/soil mites e.g Rhizoglyphus sp., Cheyletus and other predatory mites (e.g. Blattisocius, Hypoaspis) , Aleuroglyphus, Thyreophagus, Carpoglyphus , Goheira, Bryobia, Lardoglyphus.Table 3. Percentage number of mites found in the sterile trap, non-sterile trap or containerSterile trap Non-sterile trapContainer number Container1 42.54 53.73 3.7345 52 347.88 51.52 0.6143.52 55.55 0.9241.18 57.14 1.68- 96.95 3.06- 98.83 1.17- 98.79 1.297.74 - 2.2697.86 - 2.1497.53 - 2.4797.93 - 2.0398.17 - 1.83- 98.31 1.69- 99.39 0.6123456789101112131415Table 4. Animal Facility Sterile vs non-sterile attraction test resultsNumber of Mites175 168 Acarus species, 5 Tyrophagus species, 2 Glycyphagid mites500+ Acarus species, 68 Tyrophagus species, 12 Glycyphagid mites1 Tyrophagus species2 Tyrophagus species, 1 Acarus species, 1 Predatory mite500+0014Trap Number1 Area 1 (Non-sterile Trap)2 Area 1 (Sterile Trap)3 Area 2 (Non-sterile Trap)4 Area 2 (Sterile Trap)5 Area 3 (Non-sterile Trap)6 Area 3 (Sterile trap)Mite IdenticationFigure 3. Percentage trap catch with sterile vs non-sterile bait Figure 1. The BT mite trapFigure 2.Mites feeding on the food lure

Bulletin16 • June 2021 The event started as Congress always does, with the IAT AGM. This was very well attended by 91 members. A full report is available on the IAT website (www.iat.org.uk) and an abridged version is published in this issue of the Bulletin (see page 7-11). A full version of the IAT Annual Report is also available on the IAT website. Our thanks go to Robin Lovell-Badge, who was re-elected as President for the coming year and to Linda Horan (IAT CEO and Chair of Council), Simon Cumming (IAT Honorary Secretary) and Glyn Fisher (Honorary Treasurer) who all participated in the AGM.Linda HoranIAT CEO and Chair of CouncilSimon CummingIAT Honorary SecretaryThe event started as Congress always does, with the IAT AGM. ) and an abridged version is published in this issue of the Bulletin (see page 7-11). A re-elected as President for the coming year and to Linda Horan Robin Lovell-BadgeThe AGM was followed by the opening keynote speaker, Wendy Jarrett, who gave a very encouraging update on Openness within our Industry. Congress 2021 then continued over the following three weeks with presentations, workshops, trade partner sessions and poster displays.Congress 2021 then continued over the following three weeks Congress 2021 then continued over the following three weeks Wendy Jarrett

BulletinJune 2021 • 17On Wednesday 10th March, we learnt about the husbandry of venomous armoured animals. Steve Trim gave an insight into working with scorpions. Also during the ﬁ rst week, Ewan St John Smith introduced us to the fascinating world of naked mole-rats.Ewan St John SmithSaffron FosterThe second week saw presentations range from snail husbandry and oppossum care to bovines and poultry work. Natasha CartnellClaire Lathlean Eilisha Carlton Elena Mather

Bulletin18 • June 2021 During the ﬁ nal week we had the Andrew Blake Tribute Award paper which was to have been presented at AST2020, as well as the paper from the 2021 winner.Stuart Mason gave his presentation on ‘Protective cranial implant caps for macaques’. This was the winning paper from 2020. Stuart started working in the Department of Experimental Psychology at Oxford University as a manager and primate trainer in Professor Mitchell’s laboratory in 2009. Over the past ten years Stuart has trained over 100 macaque monkeys. As part of his current role, Stuart now trains researchers, technicians and support staff to work with the primates, ensuring that all involved succeed each day. Stuart delivers many presentations in his area of expertise on primate training methods and reﬁ nements at both UK and international conferences. Zoe Windsor then presented the 2021 Andrew Blake Tribute Award winning paper, ‘Reﬁ nements in head plate mouse nesting: Using composite nests to enhance welfare’.Zoe works at the Institute of Neurology University College London (UCL). She is passionate about animal welfare and chairs the UCL technician culture of care group. Over the last few years Zoe has worked closely with mice with head stages and implants and is interested in investigating reﬁ nements to improve their quality of life. Congratulations to both Stuart and Zoe – both worthy winners of this prestigiousaward. They will each be invited in person to Congress 2022 to collect their winners plaques.The ﬁ nal week of Congress also saw two presentations on Zebraﬁ sh. We also learnt about ‘Preparing, caring, sharing and ﬂ agging: tools for animal care staff’. This was followed by a very informative update by Will Reynolds from ASRU. researchers, technicians and support staff to work with the primates, ensuring Award paper which was to have been presented at AST2020, Stuart Mason gave his presentation on ‘Protective cranial implant caps for macaques’. This was the of Experimental Psychology at Oxford University laboratory Stuart Mason gave his presentation on ‘Protective cranial implant caps for macaques’. This was the Will ReynoldsCarole Wilson Chloe StevensAdrian Smith

BulletinJune 2021 • 19Congress 2021 concluded with the prize giving and the Kevin Dolan Memorial Lecture, which this year was given by Gerry Creighton, who gave a very detailed update on the elephant conservation work he has been involved with for many years. Gerry was instrumental in the breeding programme and the fantastic facilities the elephants now enjoy at Dublin Zoo, but has also moved on to work as a consultant for many other organisations. Gerry has had a long involvement with the IAT since he gave his ﬁ rst lecture at Southport in 2011 and has spoken at various Branch events in the interim. Congress would not be complete without our interactive workshops and this year was no different. Nine workshops were held over the three week period and all were very well attended. Thanks go to all presenters.Gail Davies Mark Prescott Norman Flynn Michael WilkinsonJohn Meredith Bella Williams Mollie Millington Penny HawkinsJas BarleySarah Hart-JohnsonHelen Bailey

Bulletin20 • June 2021 Thirty two posters were submitted and these were on display throughout the meeting and then remained on the Congress website throughout April. As always, the posters covered a wide range of topics, from enrichment for laboratory Zebraﬁ sh to taking blood samples from minipigs. SCAN QR CODE FOR A FULL LIST OF REFERENCESRESEARCH ANIMALSDEPARTMENTWild zebraﬁsh are found in habitats with a variety of vegetation, substrates and water ﬂow rates.SCAN QR CODE FOR A FULL LIST OF REFERENCESRESEARCH ANIMALSDEPARTMENTPlants are preferred to a barren tank.Environments containing artiﬁcial plants may contribute to lower anxiety, reduced stress response, social cohesion, learning and memory, larger brain size, improved fertility and fecundity, prevent oxidative stress, and improve ability to cope with pain.Care should be taken as plants may affect aggressive behaviour.SCAN QR CODE FOR A FULL LIST OF REFERENCESRESEARCH ANIMALSDEPARTMENTGravel, sand, or even an image of gravel is preferred to a barren tank.Environments containing substrate may contribute to lower anxiety, reduced stress response, larger brain size, larvae survival, prevent oxidative stress and improve ability to cope with pain.SCAN QR CODE FOR A FULL LIST OF REFERENCESRESEARCH ANIMALSDEPARTMENTTanks with shelter and structure are preferred to barren tanks.Structured environments may contribute to lower anxiety, reduced stress response, increased exploration, improved learning, larger brain size, and improve ability to cope with pain.SCAN QR CODE FOR A FULL LIST OF REFERENCESRESEARCH ANIMALSDEPARTMENTLive food likely provides short-term enrichment by stimulating natural predatory behaviour.NB: organisms fed to zebraﬁsh, such as rotifers or Artemia nauplii, are not considered to be sentient. Artemia nauplii, are not considered to be sentient. ArtemiaSCAN QR CODE FOR A FULL LIST OF REFERENCESRESEARCH ANIMALSDEPARTMENTGroup housing is preferred and contributes to lower anxiety and faster recovery from stress. More information is needed on preferred group sizes.Aggression may be high in small groups of zebraﬁsh (<6 individuals) so caution should be exercised and animals carefully monitored.Where ﬁsh need to be housed alone or in pairs, using clear perforated barriers to maintain visual and olfactory contact and providing other enrichment such as plants, can help reduce stress.We were once again very grateful for the enormous support shown by our trade partners. We offered the opportunity to host trade workshops, where updates on products or question and answer sessions could be held.We also offered two Flash Trade Presentation slots, where exhibitors had two minutes to entice delegates to attend their trade sessions or visit their virtual stands during Congress 2021.Linda Horan, CEO and Chair of Council, gave the closing comments to Congress 2021, ahead of the Kevin Dolan Memorial Lecture. It was the ﬁ rst Close of Congress for Linda, and who would have guessed she would be able to do it in her slippers! Jacqueline GloverJoanne MooreAnn Turner Adrian ZeltnerLouis Kitchenham

BulletinJune 2021 • 21As we were in the anniversary week of the commencement of the ﬁ rst national lockdown, Linda took a few moments for everyone to reﬂ ect on loved ones who had been lost, IAT members who had passed away and also animals used in research.Linda then went on to praise the selection of posters submitted to Congress 2021 and handed over to Elaine Kirkumof LBS to announce the Best Poster Prize winner for 2021. LBS have very kindly sponsored the Best Poster Award for many years and we are extremely grateful for this continued support.Development of a feeding device to reduce reliance onfield trials to test novel poultry red mite controlsFrancesca Nunn1, Kathryn Bartley & Alasdair Nisbet1 Moredun Research Institute, Edinburgh EH26 0PZwww.moredun.org.ukMoredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, Scotland. E-mail @mri.sari.ac.uk1) The data fro m the in vi trofeeding devices (Fig. 1) arehighly variable leading tomisleading results2) Requires invasive sampling ofhensThis strategy has 2 major drawbacks:To replace the in vitro feeding device we developed a prototype in vivofeeding device for adult mites that can be attached to the hens’ thighs forshort periods. Consi stent feeding rates of 50% and a low background mitemortality were demonstrated.Prototype in vivo feeding device Reduction: Use of the in vivo feeding device to pre-screen vaccines leads to a decreased number of hens per study (384 ‘v’ 4 per group) and less testing of suboptimal products in large field trials Refinement (improved welfare): reduces the frequency and duration of exposure to mites in field trials (50-100 mites for 3 hours/time point ‘v’ tens of thousands mites for several weeks) and less invasive blood sampling of hens. For optimal use, the device needed further development for use against all blood feeding PRM life stagesAn important innovation in Reduction and RefinementFig. 1. In vitro feeding device4Device optimisationMeshes of different aperture size, thickness and materials were tested tooptimise use for all haematophagous PRM life stages. Figure 2 shows theresults of meshes with differing aperture sizes and thickness. Feeding rateswere significantly higher for protonymphs using the 105µm aperture andthis was chosen to then study conditioning of the mites prior to feeding4.Adu lt sDeut on y m ph sPro to ny m p hs02040608010075 mM e sh% to ta l m i te s r ec ov e re d/p o uc hAdu lt sDeut on y m ph sProto n ym p h s020406080100125 m M e sh% to ta l m i te s r ec ov e re d/p o uc hAdu lt sDeu to n ym p h sPro to ny m p hs02040608010068 mM e sh% to ta l m i te s r ec ov e re d/p o uc hAdu lt sDeut on y m ph sPro to ny m p hs020406080100105 m M e sh% to ta l m i te s r ec ov e re d/p o uc hAdu lt sDeut on y m ph sPro to ny m p hs020406080100120 m M e sh% to ta l m i te s r ec ov e re d/p o uc hFig. 2. Graphs showing mite feeding rates associated with different mesh aperture sizes and thicknesses5.To determine the best treatment of mites prior to feeding assays, fourprotocols were te sted: Each mite preparation was stored at roomtemperature (RT) for 1 week and thereafter at 4oC for up to 3 weeks prior tofeeding on hens.Mite conditioningFig. 3. Percentages of mites fed following different conditioning periods: adult (a) deutonymph (b) protonymph feeding rates (c)502040608010 0% ad u lt f ee d in g r at e1 w e ek RT1 w e ek RT , 1 we ek 4oC1 w e ek RT , 2 we ek 4oC1 w e ek RT , 3 we ek 4oC02040608010 0% de u to ny m ph f ee d ing rate02040608010 0% pr ot on y mp h f ee di ng r at e• Mortality for adult mites was significantly lower after 1wk RT 3 wks 4oC conditioning than at one week RT. • Mortality for deutonymphs was not significantly different across the different conditioning points and no protonymph mortality was demonstrated. Summary Device optimised to allow all hematophagous PRM life stages to feed in vivo Compared to the in vitro device and initial in vivo prototype: Improved mite feeding rates Reduced background mortality High welfare for the hens Easily used by trained individualsReferences:1. Chauve, C. Vet. Para. 79 (1998) 239-2452. Flochlay, S., Thomas, E. Sparagano, O. Parasites and Vectors (2017) 10:3573. Bartley et al.. Vet Para. 244 (2017) 25-354. Bartley et al,. Int J Parasitol. 45 (2015) :819-8305. Nunn et al,. Vet. Para. 267 (2019) 42-46.Poultry red mites (PRM) are small, mobile ectoparasites that feed on the blood of hens. They are considered to be the most important ectoparasites in laying hens and are found in all housing systems worldwide. Infestation can cause an increase in mortality and stress behaviours1and a decrease in egg production2.Testing of novel control methods uses mites in laboratory-based tests followed by field trials. Field trials use large numbers of hens (~400) per experimental group, which are then exposed to high numbers of mites for extended periods of time3. ‘Development of a feeding device to reduce reliance on ﬁ eld trials to test novel poultry red mite controls’ by Francesca Nunn, Kathryn Bartley and Alasdair Nisbet from the Moredun Institute, Edinburgh. Congratulations to the winners, who received a monetary award of £250.The judges of the Best Poster Award also wished to highly commend two further posters:‘The jacket with pulling power – a novel approach to early-stage evaluation of magnetic nanoparticles’ by Alison Ritchie, James Dixon, Phil Clarke, Anna Grabowska at the University of Nottingham and ‘Efﬁ cacy of Medoa’s Blue Stain for the Assessment of Syphacia muris egg viability’ by Lorna Cleverly, Rebecca Lawson, Callum Logan and Michelle Powell from FERA Science Ltd.The winner of the prize was:

Bulletin22 • June 2021 Linda was delighted to announce the winner of the Animal Technology and Welfare journal Prize for 2020. This went to Cheryl Yalden for her article on ‘The relationship between employee participation and job satisfaction’.Cheryl works at King’s College London as a senior Animal Technologist, where she is actively involved in the training of new apprentices. Cheryl’s interest in the Culture of Careand staff morale were ignited during her studies for the IAT qualiﬁ cations and became the topic of her Level 6 project. The aim of the project was to explore the relationship between employee participation and job satisfaction by carrying out four workshops relating to the implementation of the 3Rs and working towards a Culture of Care with clearly deﬁ ned shared values. The full paper is open access and can be read via www.atwjournal.com.Special mention was made of the 2020 and 2021 winners of the Andrew Blake Tribute Award, and both Stuart Mason and Zoe Windsor will be invited to Congress 2022 to receive their glass plaques. The Andrew Blake Tribute Award is awarded by the Institute of Animal Technology and sponsored by the Association of the British Pharmaceutical Industry (ABPI) to an Animal Technologist at any level, judged to have made a signiﬁ cant contribution to improving laboratory animal welfare. The submission criteria for this award has been changed to make it even easier to apply. Perhaps those who have submitted a poster this year, which highlights a contribution towards reﬁ nement within animal units could be a candidate for the 2022 award. Full details of the award and how to apply can be found on the IAT website or contact the IAT Administrator (admin@iat.org.uk). The winner receives a glass plaque, a monetary award and a free attendance at Congress to present your paper.The AAALAC International Fellowship Committee have made the very difﬁ cult decision to postpone applications for the AAALAC Fellowship award for 2021. Uncertainty about international travel restrictions and also the fact that host organisations may not be able to accept visitors has meant that the Fellowship Programme has been paused for 2021.

BulletinJune 2021 • 23However, the Committee are delighted to announce that when the Programme re-opens, it will be with a new simpliﬁ ed, online application process. Details on how to apply can be found on the AAALAC website at www.aaalac.org/awards/fellowship-award as well as via the AALAS (www.aalas.org) and IAT (www.iat.org.uk) websites. Linda concluded the Close of Congress by thanking everyone for attending. Thanks were also given for the fantastic representation from our trade partners and to everyone who had presented a paper, workshop or submitted a poster. Linda thanked the Congress Committee for all their efforts in putting together the programme for Congress 2021.As always, the Congress Committee are immensely grateful to those companies who sponsored sessions and participated in Congress 2021. Special thanks go to those companies who sponsored sessions:Agenda Life Sciences Janvier LabsIPS Product Supplies LtdMMM Medical Equipment UK NKP – IsotecScionics Computer Innovation GmbHSychem TecniplastVet-TechAnd thank you again to the Prize Sponsors:Association of British Pharmaceutical Industry – Andrew Blake Tribute AwardLBS (Serving Biotechnology) – Best Poster Prize

Bulletin24 • June 2021 As well as Congress 2021, March also saw the IAT Tech Month take place. Whilst technicians could not get together as in previous years, various events and activities still took place. A full report on Tech Month will be published in a later edition of the Bulletin.Hopefully by 2022 we will all be back at face-to-face meetings. We are already busy planning a packed programme for Congress 2022 and we look forward to welcoming you to a popular venue in the North from Tuesday 29th March to Friday 1st April 2022 see page 00).Finally, a few acknowledgements from the Congress Committee. Thank you to all those who participated – whether by presenting, attending or sponsoring Congress 2021. And a huge thank you to Allan Thornhill, who despite having a fulltime job and walking the equivalent of 250 miles for Cancer Research during March, managed to keep us all on track with the virtual conference technology. We look forward to seeing you next year when we hope we can meet you all in person.Reporter: Nikki Stevens

BulletinJune 2021 • 25Congress 2022 makes a return to face-to-face meetings  Meet colleagues and friends in a relaxed atmosphere  More flexibility for attendance - book your own accommodation - select the days / meal plans / evening entertainment you wish to participate in - take full advantage of the EARLY BIRD DISCOUNT available to 31st December 2021  ‘Calls for’ PAPERS, WORKSHOPS and POSTERS announcements appear in this issue  Once you have registered, download the Congress 2022 App in order to make the most of your Congress experience INVITATION TO PARTICIPATE We’ve missed you – come and see us in 2022 ONLINE REGISTRATION OPENS 1st SEPTEMBER 2021 Venue North East England And don’t forget Ken’s Quiz makes a long-awaited return! All enquiries to congress@iat.org.uk Check for updates www.iat.org.uk

Bulletin26 • June 2021 The Andrew Blake Tribute Award commemorates the work and life of Andrew Blake, who suffered from Friedrich’s ataxia, a hereditary condition described as one of the “worst of neurological diseases”. Andrew died in May 2002 aged 39. Andrew was passionate about the need to support scientists in their work and his commitment to speaking out against animal rights activists took up much of the last ten years of his life. He died shortly before he was to collect his MBE.ANDREW BLAKETRIBUTE AWARDSPONSORED BY THE ABPIANDREW BLAKETRIBUTE AWARDDON’T KEEP YOUR GOOD IDEA TO YOURSELF!WE WANT TO HEAR ABOUT IT FOR THE 2022 AWARDDETAILS OF THE AWARD This Award is given annually, where sponsorship allows, to the Animal Technician/Technologist judged to have made the most significant contribution to improving standards in laboratory animal welfare over the previous twelve months. All qualified Animal Technologists are guided in their work by the Institute of Animal Technology’s Ethical Statement: In the conduct of their Professional duties Animal Technologists have a moral and legal obligation, at all times, to promote and safeguard the welfare of animals in their care, recognising that good laboratory animal welfare is an essential component of good laboratory animal technology and science. The Institute recognises and supports the application of the principles of the 3Rs (Replacement, Reduction, Refinement) in all areas of animal research. The Award is made to acknowledge the professional and personal commitment of Animal Technologists to improving standards in all aspects of laboratory animal care and welfare. THE PRIZE INCLUDES - CONGRESS 2022 FREE ATTENDANCE next March WHICH WILL INCLUDE PRESENTING YOUR WORK - AN ENGRAVED GLASS PLAQUE - AND £250 CASH AWARDCLOSING DATE FRIDAY 29th OCTOBER 2021 Need advice – or you wish to discuss anything regarding a possible entry? Then please email the IAT Administrator admin@iat.org.uk with your contact details and one of the organisers will respond and give you all the support you need.ARE YOU AN ANIMAL TECH?HAVE YOU BEEN PART OF A TEAM OR HAVE YOU REFINED ANIMAL CARE AND WELFARE IN YOUR FACILITY?ALL ANIMAL TECHNICIANS AND TECHNOLOGISTS, QUALIFIED AT ANY LEVEL AND PRIMARILY WORKING IN THE UK CAN ENTERSUBMISSIONS SHOULD CONTAIN AS MUCH AS POSSIBLE OF THE FOLLOWING HEADINGS AND YOU CAN INCLUDE PHOTOGRAPHS/IMAGES (THESE SHOULD BE SUPPLIED AS ATTACHMENTS):CRITERIA – The topic of work that you describe in your application may be undertaken as part of a project and presented EITHER as a POSTER / an ESSAY / a PROJECT / a SCIENTIFIC PAPER.The submission which should contain the content below must be submitted online via this link https://www.iat.org.uk/abta where you will see the Submission form for completion:- Why did you undertake this work? (what was the potential problem you were trying to improve?)- How did you undertake it? (species, numbers, sex, materials used)- Describe in a comprehensive and concise manner that allows a complete understanding facilitating reproducibility. - Explain if the work contributes to one of the 3Rs. - Explain how the welfare of the animals was improved. - Describe the results you obtained including data generated with assessment. - Were there any statistics undertaken? Please provide this information. - Acknowledgements & References. - Brief CV to include your overall contribution to the work. - Please list your supervisors or PPL holder if applicable for the work.To allow others to be able to replicate the work, please consult the ARRIVE guidelines: https://www.nc3rs.org.uk/arrive-guidelines

BulletinJune 2021 • 27 The Andrew Blake Tribute Award commemorates the work and life of Andrew Blake, who suffered from Friedrich’s ataxia, a hereditary condition described as one of the “worst of neurological diseases”. Andrew died in May 2002 aged 39. Andrew was passionate about the need to support scientists in their work and his commitment to speaking out against animal rights activists took up much of the last ten years of his life. He died shortly before he was to collect his MBE.ANDREW BLAKETRIBUTE AWARDSPONSORED BY THE ABPIANDREW BLAKETRIBUTE AWARDDON’T KEEP YOUR GOOD IDEA TO YOURSELF!WE WANT TO HEAR ABOUT IT FOR THE 2022 AWARDDETAILS OF THE AWARD This Award is given annually, where sponsorship allows, to the Animal Technician/Technologist judged to have made the most significant contribution to improving standards in laboratory animal welfare over the previous twelve months. All qualified Animal Technologists are guided in their work by the Institute of Animal Technology’s Ethical Statement: In the conduct of their Professional duties Animal Technologists have a moral and legal obligation, at all times, to promote and safeguard the welfare of animals in their care, recognising that good laboratory animal welfare is an essential component of good laboratory animal technology and science. The Institute recognises and supports the application of the principles of the 3Rs (Replacement, Reduction, Refinement) in all areas of animal research. The Award is made to acknowledge the professional and personal commitment of Animal Technologists to improving standards in all aspects of laboratory animal care and welfare. THE PRIZE INCLUDES - CONGRESS 2022 FREE ATTENDANCE next March WHICH WILL INCLUDE PRESENTING YOUR WORK - AN ENGRAVED GLASS PLAQUE - AND £250 CASH AWARDCLOSING DATE FRIDAY 29th OCTOBER 2021 Need advice – or you wish to discuss anything regarding a possible entry? Then please email the IAT Administrator admin@iat.org.uk with your contact details and one of the organisers will respond and give you all the support you need.ARE YOU AN ANIMAL TECH?HAVE YOU BEEN PART OF A TEAM OR HAVE YOU REFINED ANIMAL CARE AND WELFARE IN YOUR FACILITY?ALL ANIMAL TECHNICIANS AND TECHNOLOGISTS, QUALIFIED AT ANY LEVEL AND PRIMARILY WORKING IN THE UK CAN ENTERSUBMISSIONS SHOULD CONTAIN AS MUCH AS POSSIBLE OF THE FOLLOWING HEADINGS AND YOU CAN INCLUDE PHOTOGRAPHS/IMAGES (THESE SHOULD BE SUPPLIED AS ATTACHMENTS):CRITERIA – The topic of work that you describe in your application may be undertaken as part of a project and presented EITHER as a POSTER / an ESSAY / a PROJECT / a SCIENTIFIC PAPER.The submission which should contain the content below must be submitted online via this link https://www.iat.org.uk/abta where you will see the Submission form for completion:- Why did you undertake this work? (what was the potential problem you were trying to improve?)- How did you undertake it? (species, numbers, sex, materials used)- Describe in a comprehensive and concise manner that allows a complete understanding facilitating reproducibility. - Explain if the work contributes to one of the 3Rs. - Explain how the welfare of the animals was improved. - Describe the results you obtained including data generated with assessment. - Were there any statistics undertaken? Please provide this information. - Acknowledgements & References. - Brief CV to include your overall contribution to the work. - Please list your supervisors or PPL holder if applicable for the work.To allow others to be able to replicate the work, please consult the ARRIVE guidelines: https://www.nc3rs.org.uk/arrive-guidelines

Bulletin28 • June 2021 IAT Education on mySocietyWe are very pleased to announce that the education aspects of the new IAT database are now fully operational. This means that all applications for IAT qualiﬁ cations can be made via mySociety. MIAT, FIAT and RAnTech applications can also be made this way.If you are an existing member of the IAT, you can go to https://my.iat.org.ukIf it is your ﬁ rst visit, simply click on the ‘Log in now’ button and request a new password. You will need to use the email address we have registered for you on your account. If you have any problems requesting a new password, please contact the IAT administrator (admin@iat.org.uk) before creating a new account, as it may be that your email address on your record is different to the one you are trying to use.Once in your record, you can check your contact details – update addresses or change your password.If you are not a member of the IAT you should select the ‘Create an account’ option, and set up a new record.IAT Education on mySociety We are very pleased to announce that the education aspects of the new IAT database are now fully operational. This means that all applications for IAT qualifications can be made via mySociety. MIAT, FIAT and RAnTech applications can also be made this way. If you are an existing member of the IAT, you can go to https://my.iat.org.uk If it is your first visit, simply click on the “Log in now” button and request a new password. You will need to use the e-mail address we have registered for you on your account. If you have any problems requesting a new password, please contact the IAT administrator (admin@iat.org.uk) before creating a new account, as it may be that your e-mail address on your record is different to the one you are trying to use. Once in your record, you can check your contact details – update addresses or change your password. If you are not a member of the IAT you should select the “Create an account” option, and set up a new record.

BulletinJune 2021 • 29Registering for IAT Qualiﬁ cationsOn the lefthand side of the page, you will see ‘IAT Qualiﬁ cations’. Click on this link, and you will then see details of all the qualiﬁ cations available, along with details of providers. Registering for IAT Qualifications On the lefthand side of the page, you will see “IAT Qualifications”. Click on this link, and you will then see details of all the qualifications available, along with details of providers. If you scroll down you will see details of any qualifications which you are studying and the other qualifications available to you. Click on the “In Progress” button and you will see the status of any application or details of units so far completed. You can also download electronic versions of certificates for any qualifications completed and certified. To apply for a qualification, click on the “Click to Apply” button next to the qualification you wish to study. You will be taken to the application process. The first screen will have details of the qualification, unit titles and a link to providers who offer the qualification. Click on the “Apply Now” button. If you scroll down you will see details of any qualiﬁ cations which you are studying and the other qualiﬁ cations available to you.

Bulletin30 • June 2021 Click on the ‘In Progress’ button and you will see the status of any application or details of units so far completed. You can also download electronic versions of certiﬁ cates for any qualiﬁ cations completed and certiﬁ ed.To apply for a qualiﬁ cation, click on the ‘Click to Apply’ button next to the qualiﬁ cation you wish to study.You will be taken to the application process. The ﬁ rst screen will have details of the qualiﬁ cation, unit titles and a link to providers who offer the qualiﬁ cation. Click on the ‘Apply Now’ button. Click on the “In Progress” button and you will see the status of any application or details of units so far completed. You can also download electronic versions of certificates for any qualifications completed and certified. To apply for a qualification, click on the “Click to Apply” button next to the qualification you wish to study. You will be taken to the application process. The first screen will have details of the qualification, unit titles and a link to providers who offer the qualification. Click on the “Apply Now” button. You then work your way through the application process:– Choose your Provider.– Check your personal details.– Ensure your address is correct for posting of certiﬁ cates.– Your employer’s details.– Your application – conﬁ rming the course.– Upload any documents to support your application – proof of prior learning or previous level certiﬁ cate.– Diversity monitoring (this is anonymous and not retained by the IAT for any purposes other than supplying anonymised details to Ofqual).

BulletinJune 2021 • 31Once all sections are complete, you simply submit your application, which will be sent to the IAT administrator and your chosen provider.Your chosen provider will then contact you to discuss starting your qualiﬁ cation. If you have any queries regarding the course or prior learning it is advisable to contact the provider before submitting the application.If you have any queries or need further assistance, please contact the IAT administrator or your chosen provider.We wish you every success with your studies.Reporter: Nikki StevensAnimal Technologists – the key workers for medical researchCALL FOR WORKSHOPSl take an active part in the leading annual meeting for Animal Technologistsldo you have an area of expertise? (i.e. work with a more unusual species, bio-security, management, health & safety, been involved in a new build, environmental enrichment, GA breeding, ageing animals, transport, etc)lcould you run a 1 - 3 hour interactive workshop and qualify for a free congress?l send your ideas today on the Submission form available from www.iat.org.uklﬁnal date for submissions: Friday 29th October 2021Contact: congress@iat.org.ukCongress2022CONGRESS Invitation to Participate29th March – 1st April

Bulletin32 • June 2021 Watercolour painting from your chosen photograph.Provided fPET PORTRAITSWatercolour painting from your chosen photograph.Provided fPET PORTRAITSWatercolour painting from your chosen photograph.Provided framedAll profits going toFor more details contactwendy.steel1@outlook.comPET PORTRAITSWatercolour painting from your chosen photograph.ramed. Dimensions approx.ll profits going toFor more details contactwendy.steel1@outlook.comPET PORTRAITSWatercolour painting from your chosen photograph.imensions approx.£120ll profits going toFor more details contactwendy.steel1@outlook.comPET PORTRAITSWatercolour painting from your chosen photograph.imensions approx.ll profits going toAS-For more details contactwendy.steel1@outlook.comPET PORTRAITSWatercolour painting from your chosen photograph.imensions approx.35cm x-ETFor more details contactwendy.steel1@outlook.comPET PORTRAITSWatercolour painting from your chosen photograph.cm x25cmWatercolour painting from your chosen photograph.cmBidding will be via ASwww.animalsinscienceeducationtrust.org.uk/AND GET READY TO PLACE YOUR BIDS0NCOMING SOON!Bidding will be via ASrange of exciting lots to be wonFitBit, bungee jump, original artLook out for updates onwww.animalsinscienceeducationtrust.org.uk/AND GET READY TO PLACE YOUR BIDSAll proceeds will go to AS0N-LINE AUCTIONCOMING SOON!Bidding will be via ASrange of exciting lots to be wonFitBit, bungee jump, original artLook out for updates onwww.animalsinscienceeducationtrust.org.uk/AND GET READY TO PLACE YOUR BIDSAll proceeds will go to ASLINE AUCTIONCOMING SOON!Bidding will be via ASrange of exciting lots to be wonFitBit, bungee jump, original rtand much more!Look out for updates onwww.animalsinscienceeducationtrust.org.uk/AND GET READY TO PLACE YOUR BIDSAll proceeds will go to ASLINE AUCTIONCOMING SOON!Bidding will be via AS-ET websiterange of exciting lots to be wonFitBit, bungee jump, original artand much more!Look out for updates onwww.animalsinscienceeducationtrust.org.uk/AND GET READY TO PLACE YOUR BIDSAll proceeds will go to ASLINE AUCTIONCOMING SOON!ET websiterange of exciting lots to be wonFitBit, gee jump, original artand much more!Look out for updates onwww.animalsinscienceeducationtrust.org.uk/AND GET READY TO PLACE YOUR BIDSAll proceeds will go to ASLINE AUCTIONCOMING SOON!ET website–range of exciting lots to be wonFitBit, ee jump, original Aa rtand much more!Look out for updates onwww.animalsinscienceeducationtrust.org.uk/AND GET READY TO PLACE YOUR BIDSAll proceeds will go to AS-ETLINE AUCTIONCOMING SOON!–a wide,FitBit, ee jump, original artLook out for updates onwww.animalsinscienceeducationtrust.org.uk/AND GET READY TO PLACE YOUR BIDSETa widewww.animalsinscienceeducationtrust.org.uk/!ranBulletin16 •July 2020AS-ET NewsThe Trustees of AS-ET would like to send our best wishes to all those of you whoare having to work in difficult circumstances, ensuring your animals are cared forand essential science carries on. It is an amazing achievement to have continuedto get to work while public transport has been lacking and while avoiding beinginfected yourselves. Congratulations to all of you for demonstrating your cultureof care in a really practical way in these dangerous times. Please make sure tostill stay safe now that the restrictions have been lifted slightly – the incidence ofinfections has slowed down but the virus is still around.As you can imagine this has been a quiet time for AS-ET but we are ready forwhen circumstances allow people to get back to enrolling on courses. Our plansfor events to mark our tenth anniversary will remain on hold until the countrybecomes more settled, however remember the Congress Bursary Competition isstill running so start writing. Congress Bursary CompetitionIf you are studying for the IAT level 2 qualification or you passed it in 2018, 2019or 2020 and you are employed as a laboratory animal technician in the UK or theRepublic of Ireland you can enter the competition. This year’s topic is —‘What challenges and rewards have you experienced as an animal technician?’ Your 1,000 word essay should be submitted by 20th October 2020. Full details areon our website (www.as-et.org.uk).Patron: Professor Lord Naren Patel KTChair of Trustees: Professor Sir Richard Gardner FIAT (Hon) FRSSecretary to the Trust: Ken Applebee OBE FIAT FRSBTrustees: Stephen Barnett MSc (Hon) FRSB, Jasmine Barley MSc FIAT,Karen J Gardner, Wendy Steel BSc (Hons) FIATRegistered Charity Number 113319Registered Office: 5 South Parade, Summertown, Oxford OX2 7JLJul20:IATB NEW 10/7/20 10:02 Page 16

BulletinJune 2021 • 33 AS-ET is a charity to advance education and promote excellence in the careand welfare of animals used in science. Sponsors of AS-ET are listed below and to find out more please visit thewebsite www.as-et.org.ukAS-ET is a charity set up to advance education and promote excellence inthe care and welfare of animals used in science.To see how you can apply for a bursary visit our website (www.as-et.org.uk)The companies listed below sponsor AS-ET. If you would like to join themfind out how to support us by looking at our website.BulletinMay 2014• 15AS-ET is a charity set up to advance education and promote excellence inthe care and welfare of animals used in science.To see how you can apply for a bursary visit our website (www.as-et.org.uk)The companies listed below sponsor AS-ET. If you would like to join themfind out how to support us by looking at our website.BulletinMay 2014• 15AS-ET is a charity set up to advance education and promote excellence inthe care and welfare of animals used in science.To see how you can apply for a bursary visit our website (www.as-et.org.uk)The companies listed below sponsor AS-ET. If you would like to join themfind out how to support us by looking at our website.BulletinMay 2014• 15AS-ET is a charity set up to advance education and promote excellence inthe care and welfare of animals used in science.To see how you can apply for a bursary visit our website (www.as-et.org.uk)The companies listed below sponsor AS-ET. If you would like to join themfind out how to support us by looking at our website.BulletinMay 2014• 15AS-ET is a charity set up to advance education and promote excellence inthe care and welfare of animals used in science.To see how you can apply for a bursary visit our website (www.as-et.org.uk)The companies listed below sponsor AS-ET. If you would like to join themfind out how to support us by looking at our website.BulletinMay 2014• 15AS-ET is a charity set up to advance education and promote excellence inthe care and welfare of animals used in science.To see how you can apply for a bursary visit our website (www.as-et.org.uk)The companies listed below sponsor AS-ET. If you would like to join themfind out how to support us by looking at our website.BulletinMay 2014• 15AS-ET is a charity set up to advance education and promote excellence inthe care and welfare of animals used in science.To see how you can apply for a bursary visit our website (www.as-et.org.uk)The companies listed below sponsor AS-ET. If you would like to join themfind out how to support us by looking at our website.BulletinMay 2014• 15AS-ET is a charity set up to advance education and promote excellence inthe care and welfare of animals used in science.To see how you can apply for a bursary visit our website (www.as-et.org.uk)The companies listed below sponsor AS-ET. If you would like to join themfind out how to support us by looking at our website.BulletinMay 2014• 15AS-ET is a charity set up to advance education and promote excellence inthe care and welfare of animals used in science.To see how you can apply for a bursary visit our website (www.as-et.org.uk)The companies listed below sponsor AS-ET. If you would like to join themfind out how to support us by looking at our website.BulletinMay 2014• 15Bulletin18 •July 2020Jul20:IATB NEW 10/7/20 10:02 Page 18

Bulletin34 • June 2021 SolutionsProviderLEARN MORE AT WWW.ALLENTOWNINC.COM WWW.ALLENTOWNINC.COMOver the past 50 years our goal has been to serve science with integrity and care. With the expansion of our solutions for the laboratory animal science community we can oﬀer more support, and more choices, to help you in your quest to improve lives around the world though scientiﬁc discovery.

BulletinJune 2021 • 35SolutionsProviderLEARN MORE AT WWW.ALLENTOWNINC.COM WWW.ALLENTOWNINC.COMOver the past 50 years our goal has been to serve science with integrity and care. With the expansion of our solutions for the laboratory animal science community we can oﬀer more support, and more choices, to help you in your quest to improve lives around the world though scientiﬁc discovery.IFINTERESTEDINATTENDINGPLEASECONTACTHANNAH.EASTER@CRICK.AC.UKZOOMLINKTOBESENTBEFOREEVENTWEDNESDAY7THJULY2PMVIRTUALEVENTDAVIDBAUERANIMALMODELSTOUNDERSTANDEMERGINGCOVIDVARIANTSREBECCAFRISEFERRETSANDHAMSTERSINCOVIDRESEARCHWEST MIDDLESEX BRANCH HOW ANIMAL RESEARCH HAS HELPED UNLOCK COVID-19

Bulletin36 • June 2021 March:IATB NEW 11/7/20 06:53 Page 14

BulletinJune 2021 • 37Dispatches...UFAW announces virtual conference on recent advances in Animal Welfare Science29-30 JuneFollowing on from the success and popularity of previous conferences, UFAW is holding its eighth conference on Recent advances in animal welfare science and has once again attracted a diverse and dynamic programme of speakers from across the world.This year, the conference will again take place online over the two days. It features over 40 presentations on subjects ranging from whether ﬂ at-faced dogs are really unhealthier than other dogs to exploring oxidative stress as a potential indicator of animal welfare, and the development of tools for farmers to self-assess the welfare of their poultry and pigs in organic and outdoor systems. In addition to these talks, the conference will feature over 110 thought-provoking poster presentations covering a wide range of topics and animal species.The conference is part of UFAW’s ongoing commitment to improving animal welfare through increased scientiﬁ c understanding of animals’ needs and how best these can be met. These conferences are invaluable in bringing together scientists, veterinarians, policy makers and others from around the world to share knowledge, the latest advances and to exchange views and ideas. This virtual conference is free to attend but delegates are being asked to consider making a donation to UFAW which, as a charity, relies on donations and legacies to continue.The full conference programme, including speakers and registration details can be viewed on the UFAW website – ufaw.org.uk/conf2021.

Bulletin38 • June 2021 SITUATIONS VACANTVisit the Careers pages on the IAT Website for the latest vacancieshttps://www.iat.org.uk/vacanciesRecruitment advertising details from mail@prcassoc.co.ukAnimal Technologists – the key workers for medical researchCALL FOR POSTERSCongress2022CONGRESS Invitation to Participate29th March – 1st Aprill take an active part in the leading annual meetingfor Animal Technologistsl submit a poster – ﬁnal date Friday 4th February 2022*l choose to do an oral presentation of your posterand receive a discount – closing date Friday 17th December 2021*l send your ideas today on the Submission form available from www.iat.org.uk (*posters will notbe accepted for display at Congress unless theyhave been properly submitted and approved bythe Congress Committee)l two best posters will receive a prize based on the criteria: environmental enrichment / scientiﬁc basisl plus claim up to 10 CPD points – details given on acceptance of submissionContact: congress@iat.org.uk

BulletinJune 2021 • 39Front cover: The way we were Courtesy: Carla Foley DIARY DatesEvents/Dates subject to change due to the current quarantine situation and possible ongoing restrictions.4,7,11,18 JuneLooking Back, Reaching Forward60th CALAS/ACSAL Annual Symposium Online. Details: https://calas-acsal.org/site/symposium/annual?nav=sidebar9 JuneClosing date Times Higher Education Technician of the Year Award Details: https://www.technicians.org.uk/times-higher-education-awards-20219 JuneNIO Virtual WorkshopThe Learning CurveDetails: Info@LearningCurveDevelopment.co.uk9 JuneIntroduction to Microbiological Monitoring in Rodents FacilitiesFondazione Guido Bernardinihttps://www.fondazioneguidobernardini.org/en/programs/online-course-introduction-to-microbiological-monitoring-in-rodents-facilities.html15 JunePrinciples of training, mentoring and assessing virtual workshopDetails: Info@LearningCurveDevelopment.co.uk27 JuneIn aid of the Grifﬁ n InstituteLondon Bridges WalkDetails: http://grifﬁ ninstitute.org.uk/bridges29-30 JuneUFAW Animal Welfare ConferenceDetails: Page 37To View NowEfﬁ cient management of genetically altered mouse coloniesNC3Rs/Mary Lyons CentreTo view: https://vimeo.com/ 518501136/ 2fc46ddd3e?utm_campaign=March+2021&utm_medium=email&utm_source=govdelivery7 JulyWest Middlesex Branch Virtual EventDetails: Page 35

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