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Apr17_3 720311940 e S IATB NEW 13 4 17 08 05 Page 1 Institute of Animal Technology Bulletin G G G Named Persons Workshop Report Congress Poster Abstracts AS ET G G G Branch News Situations Vacant Diary Dates Vol 53 No 4 April 2017

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Apr17_3 720311940 e S IATB NEW 13 4 17 08 05 Page 3 Bulletin Vol 53 No 4 April 2017 EDITOR Sarah Lane bulletineditor iat org uk ASSISTANT EDITOR Carole Wilson bulletinassistant iat org uk ADVERTISEMENT MANAGERS PRC Associates Ltd mail prcassoc co uk Published monthly by the Institute of Animal Technology Registered Office 5 South Parade Summertown Oxford OX2 7JL CONTENTS Named Persons Workshop Report AAALAC Fellowship Application Obituary Keith Millican Steve Moore Memorial Poster Prize 5 8 9 10 11 13 Congress 2017 Poster Abstracts 14 17 Suppliers Register i xliv AS ET 18 20 IAT Logo Competition 22 25 Branch News 26 28 Situations Vacant 29 30 ISSN 0263 2861 For enquiries other than Bulletin related contact IAT ADMINISTRATOR admin iat org uk or 0800 085 4380 Final copy date for June Bulletin 1st May The opinions expressed in the Bulletin do not necessarily reflect those of the Editor or the Institute Diary Dates 31

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Apr17_3 720311940 e S IATB NEW 13 4 17 08 05 Page 5 Bulletin April 2017 5 Named Persons Workshop T he IAT LASA and LAVA held another joint Workshop on 26th January 2017 This was following on from the publication of The Guiding Principles for the NEW Named Person roles NTCO NIO and HOLC We welcomed 100 delegates to the Workshop which was aimed specifically at those persons holding individual or combined roles and the day consisting of a series of presentations by experienced practitioners with in put from the Home Office David Anderson IAT and LASA Vice president was the chair for the event and he welcomed everyone to the meeting The first presenter Adrian Deeny from UCL delivered a talk on the Establishment Licence Holder s perspective on the roles of the Named Persons He explained that the named persons roles should make it easier for everyone to comply with the law Researchers should see named people as part of the team reducing the possibility of a two tribes culture us and them They should also have the confidence and backing to report and deal with issues The Key messages Named Persons have key roles in ensuring that the Establishment Holder fulfills his her responsibilities Named Persons are an essential part of the research effort and the research team Named Persons should be seen to be supporters of research not obstacles to it Named Persons should make it easier to comply than not comply with ASPA Named Persons are the animal s champions Mel Leech and Maggie Lloyd delivered a session aimed at NTCOs entitled Developing assessments and defining competence of practical skills

Apr17_3 720311940 e S IATB NEW 13 4 17 08 05 Page 6 6 April 2017 Bulletin Mel is relatively new at Edinburgh University and shared her vision for training records that ensure quality and consistency of individuals training We looked at what exactly is competence Records should give levels of ability not just be a series of ticks If you find a common problem this is a good place to start improvements as the more people involved the more opportunity there is to refine Key points to have defined

Apr17_3 720311940 e S IATB NEW 13 4 17 08 05 Page 7 Bulletin April 2017 Maggie from Red Kite Veterinary Consultants then split us into groups where we took on roles of trainer trainee and assessor making paper aeroplanes as the example This exercise was to emphasise the importance of the detail required on a competence checklist Anja Petrie and Mary Mowat from the University of Aberdeen delivered a presentation aimed at NIOs called Data and information collection They gave the audience lots of hints and tips on free databases DBALM for Alternatives PUBMed google scholar and Norecopa How using MeSH terms is a controlled vocabulary of pre defined terms which can refine an internet search Some resources experimental design Experimental Design Assistant EDA by the NC3Rs G G 3Rs Reduction co uk by M Festing www 3rs reduction co uk http 3rs ccac ca en research reduction experimental design html Alternatives Research Centres G http caat jhsph edu http altweb jhsph edu G https cms uni konstanz de leist caat europe Funding G http www drhadwentrust org about us who we are G http www frame org uk Tissue sharing G http www sharmuk org tissue list search Data bases G http oslovet norecopa no fag aspx fag 57 mnu databases_1 G https ec europa eu jrc en scientific tool database alternative methodsanimal experimentation G http toxnet nlm nih gov altbib html G http www nkca nl algemeen top_menu english G http www atla org uk Examples for refinements NC3Rs RSPCA severe suffering Norecopa 7

Apr17_3 720311940 e S IATB NEW 13 4 17 08 05 Page 8 8 April 2017 Bulletin What are MeSH terms G G G G a controlled vocabulary of pre defined terms MeSH Medical subject headings created by the National Library of Medicine used for searching certain databases namely Medline PubMed and also use by the Cochrane Library hierarchical system of structure phrases Kathy Ryder a current Home Office Inspector had put together a presentation outlining some of the more common problems she encounters for the Named Persons problem page The Home Office understand that establishments implement the Named Persons roles differently but the key to them all being successful is clear communication and structure Time to undertake all expectations of the Named Person role is the limiting factor for many individuals especially the NIO role After a very nice lunch there was a HOLC session presented by Caroline Chadwick and Val Summers who discussed how the role is to ensuring Home Office working practices and policies are delivered The HOLC must ensure that systems are in place to deliver and exchange Home Office information effectively Finishing off the day we had an interactive workshop with an opportunity to give feedback on and improvements to the current version of the LASA LAVA IAT Guiding Principles for NTCO NIO and HOLC working under the Animals Scientific Procedures Act 1986 published in 2016 In general the feedback was very positive and supportive of the document in providing definition and context for role holders to be effective in their approach and operations In summary the Named Persons Workshop 2017 was an excellent event for providing an update on how the NTCO NIO and HOLC roles are being implemented across the UK some considerations for how they may be refined and an opportunity to network with colleagues in those roles to learn from experience Copies of all the individual presentations can be found on the IAT website http www iat org uk nacwo Linda Horan

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Apr17_3 720311940 e S IATB NEW 13 4 17 08 05 Page 10 10 April 2017 Bulletin OBITUARY KEITH MILLICAN Keith Millican from his 3 minute interview published in the May 2015 Bulletin K eith died on 16th February 2017 after a long and debilitating illness Keith was an active member of Council for many years concerning himself primarily with the education of animal technicians a cause dear to his heart Keith had wanted to write a history of the IAT because he wanted younger members to realise what some of our earlier struggles were about In the

Apr17_3 720311940 e S IATB NEW 13 4 17 08 05 Bulletin Page 11 April 2017 11 early 20th century laboratory animals were cared for in a perfunctory manner by anyone who happened to be handy the boiler man or the night watchman Then scientists realised that if they wanted reliable results reliable animals were needed Thus the push to get educated people to produce these reliable animals happened backed by the Medical Research Council that produced the ATA later becoming the IAT Keith watched this happening in the early 50s and was soon busy helping with the process He joined the Examination Board where he helped to develop the syllabuses and thus the extension of the scope of the examinations The IAT has cause to be proud of and grateful for the continuous effort Keith made to improve the status and education of animal technicians Dorothy P Woodnott O O O Keith spent many years as Senior Chief Laboratory Scientific Officer at the National Institute for Medical Research NIMR Following his predecessor Doug Short Keith modernised and implemented many professional practices for the animal technologists that cared for the animals and introduced the concept of Specific Pathogen Free SPF for a number of species including the cat colony Keith also taught during the 1970s and 80s in the evenings and held at NIMR many animal handling and sexing practicals for the students at all levels studying under Dr Margaret Dinsley at Barnet College of Technology Many students were exposed to handling a number of uncommon species including the extremely nervous cotton rats Keith always encouraged technicians at NIMR to move around the animal units there which gave a lot of technicians a wide knowledge of different species and skills and would often pop into units to say hello to the technicians working in there He was a colourful character and recounted many interesting stories to all those he met and taught both at work and at Barnet College

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Apr17_3 720311940 e S IATB NEW 13 4 17 08 05 14 April 2017 Page 14 Bulletin The following seven poster abstracts were submitted in time to be displayed at Congress last month but missed the deadline to be included in the Congress Handbook The full posters will be published in forthcoming issues of ATW DNA sampling by skin swabbing for Zebrafish Danio rerio and sticklebacks Gasterosteus aculeatus Carl Breacker University of Leicester Department of Neuroscience Psychology and Behaviour University Road Leicester LE1 7RH Fin clipping from live fish under anaesthetic is typically used to sample DNA for subsequent PCR analysis However fin clipping which has the potential to alter fish behaviour and increase the risk of infection is time consuming and requires extensive training The potential of skin swabbing for DNA sampling has been validated in a small number of studies as an effective alternative method but has yet to become a widely accepted protocol Here we describe the technique and compare DNA recovery from fin clips and swabs for two species Zebrafish and three spined sticklebacks We find that both methods are sufficient for successful PCR analysis O O O Social interaction for pregnant New Zealand White rabbits on reproductive toxicology studies John Holmes Envigo Barric Lane Occold Eye Suffolk IP23 7PX For many years rabbits on reproductive toxicology teratology studies have been housed individually We have been looking at ways to successfully allow social interaction between pregnant non sibling rabbits While we continue to

Apr17_3 720311940 e S IATB NEW 13 4 17 08 05 Bulletin Page 15 April 2017 15 evaluate permanent pair housing we considered whether providing social interaction during grouped exercise periods would be beneficial to our study animals This poster discusses the findings of the trials that we have conducted O O O Combined screening strategy to reduce the numbers of sentinel animals used whilst maintaining confidence in results Andrew Dickinson Surrey Diagnostics Ltd PO Box 156 Cranleigh Surrey GU6 8ZU In recent times there has been a considerable focus on PCR RT PCR being sold as the gold standard in laboratory animal health monitoring This however is sadly not the case Whilst these molecular screening methods are very useful in the right situation the use of PCR alone in the context of animal disease detection does not provide all the information required as with any method when used on its own any gaps in data are potentially problematic What we propose here is a combined approach to laboratory animal health monitoring where both the traditional methods serology microbiological culture and microscopy are used alongside molecular methods to give more confidence in results and a more rounded picture of a colony s health status whilst at the same time allowing a significant reduction in the number of sentinel animals used O O O Sarah Tamasauskas Agenda Life Sciences PO Box 24 Hull East Riding of Yorkshire HU12 8YJ Citrobacter freundii is a human pathogen that is routinely carried within the human intestine and is not usually a concern to healthy individuals It is not routinely found in rodents and is not routinely screened for While performing our routine health screening results showed that we had a positive test for Citrobacter freundii This positive result was initially thought to have been brought in from a member of staff but upon further investigations it was seen to be bought in from an outside animal source Measures have been tightly put in place to prevent the spreading of the infection to the other animals within the facility These include a quarantine

Apr17_3 720311940 e S IATB NEW 13 4 17 08 05 16 April 2017 Page 16 Bulletin area autoclaving of all cages within the area and the treatment or animals that are due to stay within the unit for longer than one week O O O Target training pigs within an isolation unit a pilot study Louise Carder The Pirbright Institute Ash Road Pirbright Woking Surrey GU24 0NF Training is an important tool which can be used to reduce fear and distress that may occur as a result of catching restraining and carrying out regulated procedures on protected animals We undertake much of our large animal work in high containment animal isolation units Within this high containment environment we have started to undertake target training using positive reinforcement in pigs with the aim to improving the welfare of the animals housed in our facility and thus the quality of the research conducted The training process consists of using target training along with positive reinforcement to guide pigs into a weigh crate The training was successful and we hope to be able to use it with regulated procedures and also with other species O O O Improving safety assessment studies with the introduction of a refined ECG vest for dogs Peter Fisher GlaxoSmithKline David Jack Centre for Research and Development Park Road Ware Hertfordshire SG12 0DP ECG recording in conscious unrestrained animals is a common non invasive method of collecting cardiovascular data This method improves on the snap shot recordings that require dogs to be restrained allows limited data collection and increases stress Data capture is achieved by placing the dog in a canvas jacket holding an external transmitter attached to ECG pads on the dog s chest Dogs are unrestrained within their normal home environment for 22 hours However we noticed a high number of incidences of wires becoming accessible for the dog to chew preventing full data capture leading to repeated data capture sessions To combat this we designed a bespoke vest to prevent access to the wires

Apr17_3 720311940 e S IATB NEW 13 4 17 08 05 Bulletin Page 17 April 2017 17 This poster will give an overview of the design phases and how the new version has reduced the need for repeated capture of cardiovascular effects on safety assessment studies and how the welfare of the dogs has been improved O O O Refinements in housing during data capture in long term safety pharmacology dogs Chas Bailey GlaxoSmithKline David Jack Centre for Research and Development Park Road Ware Hertfordshire SG12 0DP When collecting ECG data dogs are individually housed to avoid cross talk between transmitted signals over a 24 hour recording period Dogs are cohoused between recording periods between studies therefore separation for studies can introduce additional stress In spite of robust temperament testing regimes enabling a selection of dogs with the ability to cope with long term laboratory housing and long periods of individual housing technicians noted increases in separation anxiety behaviours for example increased pacing In 2016 we upgraded to the new digital telemetry system enabling collection of cardiovascular data from pairhoused dogs as signals now transmit over different frequencies potentially reducing stress As part of a validation study monitoring and observing dogs via video cameras and routine visual checks from technicians was incorporated The results of this validation study will be discussed and why this refinement has removed the need for individual housing to collect cardiovascular data The Congress Committee wishes to apologise and regrets any inconvenience to Ruth Ferguson as author of the poster display 3Rs improvements in the BEXSERO Meningitis B vaccine potency test and Lisa Johnson who submitted it at Congress for the oversight inadvertently publishing their wrong place of work on p63 of this year s Handbook Ruth and Lisa are both employed by National Institute for Biological Standards and Control NIBSC Blanche Lane South Mimms Potters Bar Hertfordshire EN6 3QG

Apr17_3 720311940 e S IATB NEW 18 April 2017 13 4 17 08 05 Page 18 Bulletin Patron Professor Lord Naren Patel KT Chair of Trustees Professor Sir Richard Gardner FIAT Hon FRS Secretary to the Trust Ken Applebee OBE FIAT FRSB Trustees Stephen Barnett MSc Hon FRSB Jasmine Barley MSc FIAT Karen J Gardner Registered Charity Number 113319 Registered Office 5 South Parade Summertown Oxford OX2 7JL An AS ET day out the IAT West Middlesex Annual Trade Show and Technician Symposium They came from far and wide and descended on London in great numbers no not the traditional London sparrows but animal technicians for what has become an annual phenomenon the West Middlesex Technicians Symposium and Trade show The Branch fervently believes in supporting career development and the aim of the symposium is to give technicians particularly younger technicians the opportunity to gain new experiences and expand their knowledge One of the main objectives of AS ET is advancing education and promoting excellence in the care and welfare of animals used in science AS ET has distributed or is committed to distribute nearly 85 000 in grants to support education and welfare activities The bulk of the grants are in the form of bursaries to enable individuals to attend courses to improve their own knowledge and skills These courses range from specialist short courses to long formal courses such as those leading to the IAT further and higher education qualifications Prize Judges Fraser Darling representing IAT West Middlesex Branch David Gregory representing Charles River and Jas Barley representing AS ET

Apr17_3 720311940 e S IATB NEW 13 4 17 Bulletin 08 05 Page 19 April 2017 19 In preceding years the West Middlesex branch has been a major supporter of AS ET as the aim of the symposium matches the charity s aims Sponsored by Charles River UK Ltd a prize for the best young presenter was offered and AS ET were delighted to be able to assist by providing one of the three judges To say that the judges were spoilt for choice is a understatement and the four qualifying young presenters made it very difficult to decide on a winner However after lengthy debate the judges decision was to award the prize to Emma Rowley of Imperial College for her presentation on the Environment Enrichment Committee It is hoped that all the presentations from the symposium will be published in due course in the IAT Journal Animal Technology and Welfare In addition to participating in the judging for the prize the AS ET stand was part of the trade exhibition and Steve Barnett was handling a steady stream of enquiries throughout the day on the work of the AS ET Particular interest was shown in the two competitions which enable the winners to attend either IAT Congress or the American Association for Laboratory Animal Sciences National Meeting in the USA The eagerness of all the delegates to gain as much from the day was extremely reassuring and I enjoyed the opportunity to meet some of the younger technicians If those attending the meeting are anything to go by I think it is safe to say that the future of our industry is looking bright Jas Barley AS ET Trustee Wendy Steel receiving the prize on behalf of Emma Rowley from David Gregory Charles River UK Ltd

Apr17_3 720311940 e S IATB NEW 20 April 2017 13 4 17 08 06 Page 20 Bulletin AS ET is a charity to advance education and promote excellence in the care and welfare of animals used in science Sponsors of AS ET are listed below and to find out more please visit the website www as et org uk

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Apr17_3 720311940 e S IATB NEW 13 4 17 08 06 Page 22 22 April 2017 Bulletin IAT Logo Competition Winner Announced A s many of you will be aware we have been holding meetings with the Youth Reps over the last couple of years and it was suggested that the IAT should look into producing merchandise for the purposes of promotion The items that were suggested were a hoodie polo shirt and lanyards It was then suggested that we could run a competition for our members to design a new logo for the IAT merchandise During the development of the new IAT Careers Brochure we introduced our new tag line of A Caring Career which we could then use in conjunction with the new logo to print onto the merchandise This was approved by Council and with the help of Elaine Kirkum we sourced the hoodies polo shirts and lanyards All we needed then was a winning design The new merchandise would then become an important contribution to the IAT technician month as we could use them as prices for the various competitions being run The logo competition was launched in October 2016 to find a design that we could use on our merchandise IAT Logo Competition The IAT are looking for a new mouse logo to replace the one shown here and it will be used to publicise the IAT on its merchandise This design will feature on its hoodies polo shirts bags pens etc Entries are invited for the official logo competition and all entries need to be submitted by the 30th November 2016 The winning entry will be decided at the December IAT Council meeting The winning entry will also have their design on all the IAT merchandise and also will receive an IAT hoodie polo shirt and other merchandise displaying their logo This competition is open to all members of the IAT The winning logo will become a symbol of the IAT and will be showcased at events and featured in future promotional materials Entries should be sent to communication iat org uk

Apr17_3 720311940 e S IATB NEW 13 4 17 Bulletin 08 06 Page 23 April 2017 The closing date for the logo competition was the 30th November and all designs were judged by Council at their meeting held on the 6th December We had 10 entries in total and here are a selection of them Amie Bret Harwell Sandra Sprat Edinburgh University Kelly Woodhouse Red Kite Annie Barling Envigo Annie Barling Envigo Annie Barling Envigo 23

Apr17_3 720311940 e S IATB NEW 24 April 2017 13 4 17 08 06 Page 24 Bulletin I would just like to say on behalf of IAT congratulations to all the entrants for their designs and also to the Youth Reps for being involved in the creation of this idea After an anonymous vote there was a clear winning design which was sent in by Annie Barling from Envigo in Oxfordshire

Apr17_3 720311940 e S IATB NEW Bulletin 13 4 17 08 06 Page 25 April 2017 25 Once the winning logo had been decided it was time to see what the finished article would look like We then received the initial design from the print company for us to review Council and the Youth Reps agreed that this looked really good so it was time to run off a couple of samples to look at and these were the finished product So if you want to purchase a hoodie or a polo shirt with the new logo then visit the IAT website http www iat org uk I would once again like to thank everyone that has been involved and to all the entrants for their designs Adrian Woodhouse Communications Group

Apr17_3 720311940 e S IATB NEW 26 April 2017 13 4 17 08 06 Page 26 Bulletin North East of England Branch Symposium 8th March 2017 FERA York IT S OFFICIAL THE SUN DOES SHINE IN YORKSHIRE occasionally and it was certainly shining when 57 delegates and 30 trade representatives congregated in the splendid surroundings of the FERA facility located just outside York for the 2017 NEE Branch Symposium and AGM Previous events have been held at Sheffield University Newcastle University and York University so our host for the day Andy Cunningham needed to be at the top of his game to live up to expectations The early signs were promising plenty of coffee for arriving delegates and a seamless check in process provided by Rachel and Jo from Sheffield The format for the day was mingling on arrival followed by a full programme of talks punctuated by lunch and opportunity to peruse the trade stands Finally the branch AGM would be held once the business of the day was concluded The first talk was by Julia Coates of FERA who spoke about trapping and health screening of wild beavers living along the River Otter in Devon I ll be dammed sic if I even knew we had wild beavers in England Perhaps they are just lodging Second up was bee expert Selwyn Wilkins also of FERA who spoke about novel methods of assessing the effects of pesticides on various

Apr17_3 720311940 e S IATB NEW Bulletin 13 4 17 08 06 Page 27 April 2017 27 species of bee I could make some cheap gags about hives of activity or stings in the tail but that would just make me look like a bumbling idiot Gareth Reedman of Plymouth University took the third slot with a talk on the use of wild fish including how they are microchipped and tracked and their use in refining Schedule 1 techniques There was also reference to the impact of plastics on the marine environment thought provoking stuff The final presentation of the morning session was a fascinating talk by Alexandra Robinson and Hayley Forest from Cambridge University who spoke about naked mole rat husbandry The colony has unusual husbandry requirements and the facility has refined the dietary provision and changed the lighting regime to make the environment more natural for the animals We then broke for lunch with the opportunity to visit the 20 trade stands from companies supporting the event New innovative products ensure that the UK research industry leads the way in welfare techniques It is always good to catch up with comrades colleagues and competitors alike and to keep up with the industry merry go round Lunch was excellent Mr Cunningham s support team played a blinder and there was plenty to satisfy even the most gluttonous delegate Reconvening after lunch we had an insightful talk by Robyn Grant of Manchester University who spoke about whiskered animals and how they make use of these specialised hair growths to analyse their environment Seals mice rats and others have their own pattern of whiskers which are directly mapped onto particular areas of their brains helping them to navigate hazards without suffering injury The sixth talk by Jo Dorning of Bristol University

Apr17_3 720311940 e S IATB NEW 28 April 2017 13 4 17 08 06 Page 28 Bulletin concerned hierarchy within urban fox populations and what happens when individuals fall from their position of dominance A life lesson for us all Kyle Martin of Sheffield University stepping in for his colleague Gareth Fraser gave the penultimate talk about the regeneration capabilities of sharks teeth and what lessons we humans can take from this process We all learned the differences and similarities between teeth and denticles so feel free to ask any Committee member for a full explanation The final presentation was by Harry Isaacs from York University who gave an overview of husbandry techniques and experimental use of Xenopus particularly their application in developmental studies Very interesting especially for those who spend their days working with rodents After the last talk there was another chance to visit the trade stands and for those not attending the AGM to have a final coffee or OJ before they left Our thanks go to FERA and Andy for hosting the day and to the catering staff for putting on a splendid spread Particular thanks to all the presenters for sharing their knowledge so enjoyably and a special mention to Rachel and Jo from Sheffield University and Judith from Agenda for making the whole day happen Another successful Symposium keep it up NEE Branch Glyn Selway Branch Reporter

Apr17_3 720311940 e S IATB NEW Bulletin 13 4 17 08 06 Page 29 April 2017 29 IAT Branch Raffle at Congress 2017 Following the draw of the IAT Branch Raffle at Congress there were three prizes left unclaimed If one of these is yours please contact the IAT Administrator at iat101 btconnect com to claim your prize Orange edged raffle ticket number 52 AB 952896 Wins an Amazon voucher donated by the West Middlesex Branch Red edged raffle ticket number 152 AB 748783 Wins a Currys PC World voucher donated by the London Branch Orange edged raffle ticket number 50 AB 952896 Wins a bag of chilli sauces donated by the Norfolk Suffolk Huntingdon Branch

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Apr17_3 720311940 e S IATB NEW 13 4 17 08 06 Bulletin Page 31 April 2017 31 DIARY Dates 18 20 April 2 4 May PiL A C Training London Details from info learningcurvedevelopment co uk 15 June AWERB UK Putting Ethics into the AWERB Central London Details from research animals rspca org uk 4 May North West Branch Steve Moore Memorial Poster Prize Details from nicky datesand com See page 25 15 16 June NACWO Course Cambridge Details from info learningcurvedevelopment co uk 1 June Closing date for applications for AAALAC Fellowship See page 9 26 June 7 July International Course in Laboratory Animal Science Utrecht Details from las uu nl 7 June Tecniplast Foundation Course in Tecniplast IVC s Central UK Details from info tecniplastuk com 30 June BRET Closing date for proposals Details from bretenquiries btinternet com 8 June Tecniplast IVC Knowledge and Skills Refresher Course Central UK Details from info tecniplastuk com 6 September LASA 3Rs UFAW Planning and Implementing the 3Rs South of England Details from www lasa co uk meetings Cover photo Rhino Heather Warnock

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