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Institute of Animal Technology Bulletin G G G IAT Council Meeting Zebrafish Husbandry Meeting We would like you to Review G G G Branch News NIO Forum Diary Dates Vol 54 No 7 July 2018

Bulletin Vol 54 No 7 July 2018 EDITOR Carole Wilson bulletineditor iat org uk ASSISTANT EDITOR Karen Dunford bulletinassistant iat org uk ADVERTISEMENT MANAGERS PRC Associates Ltd mail prcassoc co uk Published monthly by the Institute of Animal Technology Registered Office 5 South Parade Summertown Oxford OX2 7JL ISSN 0263 2861 For enquiries other than Bulletin related contact IAT ADMINISTRATOR admin iat org uk or 0800 085 4380 Final copy date for September Bulletin 1st August The opinions expressed in the Bulletin do not necessarily reflect those of the Editor or the Institute CONTENTS Dear Readers IAT Council Meeting 5 7 10 MIAT Awards 11 Congress 2019 13 Michael Coiro Obituary 17 Zebrafish Husbandry Meeting 18 21 WAS Conference Zebrafish 24 25 AS ET 27 29 We would like you to Review 31 33 The Photo Competition 34 37 The Cartoon Winner LARN Meeting Report 39 40 42 BING 43 Branch News 44 NIO Forum 45 Diary Dates 47

Bulletin July 2018 De ar Re aders A whole year has passed since I took over editorship of the Bulletin and now this is my second July edition A year ago I introduced myself and now I find myself having to make a couple of apologises Firstly I would like to apologise to Michala Nightingale for attributing her photo of a robin on the cover of the May edition to Ken Lovelock Ken pointed out to me that he had not taken the picture but this month it is his image of a kingfisher on the front cover as the winner of the photographic competition voted for at Congress in March My congratulations go to Ken the image is stunning I would also like to compliment Michala on her photos which were also amazing and covered a wide variety of species More images from the photographic competition on pages 34 37 My second apology is the incorrect dates for the LASA conference in the June edition somehow I muddled them up with the AALAS conference I have corrected this in diary dates page 47 Elsewhere in the Bulletin this month are meetings covering both large and small animals read the LARN report on pages 40 42 for large animal information If you are more interested in small animals zebrafish in particular then read the Sheffield Zebrafish Husbandry Day report on pages 18 21 I was at that and we all had a fantastic time and the heavy snow made it an adventure to get home Karen has also written a brief article about how to write a review for the Bulletin pages 31 33 so no excuses now get writing And Keep Reading Carole 5

Bulletin July 2018 7 IAT Council meeting synopsis held on Wednesday 16th May 2018 New Council Members At the AGM in March four new Council members were elected to serve a threeyear term Three of the new council members Nathan Hill John Waters and Alan Palmer were welcomed to their first Council meeting and invited to briefly introduce themselves The fourth newly elected member Sylvie Mehigan was unable to attend the meeting Election of Council Officers Council Officers are elected every year to the various posts on Council Elections took place at the May Council meeting with the following results Chair of Council CEO Vice Chair of Council Honorary Secretary Honorary Treasurer Assistant Treasurer Board of Educational Policy Chair Secretary Board Members Board of Moderators Chair Secretary Ken Applebee Linda Horan Linda Horan Charlie Chambers Glyn Fisher Glyn Fisher Steven Cubitt Haley Daniels Adele Kitching Haley Daniels Simon Cumming Registration Accreditation Board Chair Ken Applebee Board Charlie Chambers Glyn Fisher Cathy Godfrey John Gregory Wendy Steel Stuart Stevenson Observers Charles Gentry Certificate Holders Forum Kathy Ryder Home Office Ngaire Dennison LAVA LASA Congress Committee Chair Alan Graham

8 July 2018 Committee members Animal Welfare Group Chair Secretary Group members Bulletin Haley Daniels Adele Kitching John Waters Linda Horan Allan Thornhill Matt Bilton Simon Cumming Kally Booth Nicky Gent Sylvie Mehigan John Waters Lois Byrom co opted Chair of ATW Bulletin Editorial Board and Editor of ATW Jas Barley Editor Bulletin Deputy Editor Bulletin Carole Wilson Karen Dunford co opted Communications Group Chair Group members Adrian Woodhouse Nathan Hill Elaine Kirkum Theresa Langford Sylvie Mehigan Allan Thornhill Lynda Westall Branch Liaison Officer Deputy Branch Liaison Officer CPD Officer Diversity and Equality Officer Lynda Westall Kally Booth Charlie Chambers Haley Daniels UK Biosciences ASG Co ordinator Representatives Charlie Chambers Steve Owen Alan Palmer EFAT Co ordinator Deputies Charlie Chambers Glyn Fisher John Waters Website Electronic Media Deputy Allan Thornhill Sam Jameson Education The electronic Notifications of Performance were sent out in May Education diplomas were due to be produced at the end of May

Bulletin July 2018 9 Some time ago the RSPCA LASA and IAT had held a joint Transgenic Training Workshop Surplus funds from this meeting are now due to be returned to the three organisations and IAT Council agreed that the money should be used for an educational purpose It was then agreed that the next 200 students who register for the IAT Level 2 Diploma will receive a complimentary copy of the Introduction to Laboratory Animal Science Technology and Welfare manual ILASTW Competency Assessment Workshop A repeat of the Competency Assessment Workshop held in January 2018 will be held on 10th July This meeting will once again be a joint meeting with LASA Animal Technology Welfare The April issue of ATW was published ahead of schedule so that it was available at Congress 2018 The Editorial Board extended its thanks to Adele Kitching for ensuring all copies of the posters presented at Congress were forwarded to the ATW Editor for future publication Appropriate papers from Platform Presentations will also be published The Journal Prize for 2017 was awarded to Sarah Lawton for her paper Do buccal swabs from zebrafish give enough of a sample of DNA to be used as a viable non invasive method of genotyping The AS ET Tech 2 Tech Article prize was awarded to Emma Billingham for her article The Curious Tale of Axolotl Enrichment At the AGM a query was raised as to whether the IAT Council would review the packaging currently used for postage of ATW and Bulletin This was briefly discussed at the Council meeting and various options will be sourced for the July meeting following which a decision will then be made on future packaging Animal Welfare Group The Animal Welfare Group held a meeting at Congress 2018 to agree a focus for the next twelve months The focus will be on Non compliance due to lack of food and water Over production of GA rodents European Federation of Animal Technologists EFAT have been invited to attend an Expert Working Group on Genetically Altered Animals and representatives have also been invited to attend the SPCAL meeting in Portugal to promote animal technologist training across Europe

10 July 2018 Bulletin Communications Group The Communications group reported that 36 entries had been received for the IAT Photo Competition The top 20 photographs as chosen by IAT Council had been displayed at Congress 2018 and delegates asked to cast their votes for their favourites Winning entries will be used on the cover of the Bulletin and ATW so look out for them on future issues The Communications Group are also looking forward to a Technician Week in March 2019 with further details available on the website and in future Bulletin issues Branch Liaison A Branch Reps meeting had been held prior to the May Council meeting with nine Branches represented Guidance was requested for GDPR as well as more information on the website Branch Calendar The Branch Raffle at Congress 2018 raised 975 for the Brains Trust Charity Council rounded this up to 1250 and the Brains Trust are exceedingly grateful for this donation Annual Branch grants will also be paid this month There had been discussion at the March Council meeting about the name of the Youth Reps Group It was agreed that the name did not reflect current diversity requirements and so the Group were asked to consider a new name It was eventually agreed that the group would be called the Branch Innovation and Networking Group or BING as this reflects the nature of the group and its more inclusive membership Congress 2018 A full report on Congress 2018 and the feedback survey were published in the June issue of the IAT Bulletin It was agreed that Congress 2018 was again very successful with a record number of attendees for recent years The app was enhanced to allow delegates to register themselves on arrival and print off their own badges 98 of respondents to the feedback survey found the app easy to use GDPR The IAT have produced an updated Privacy Policy and have emailed all IAT members regarding receiving IAT communications Branches will also need to contact their members to ask if they wish to remain on their mailing lists The next meeting of the IAT Council will take place in July 2018

Bulletin July 2018 MIAT Awards IAT Council are delighted to announce that the following members have upgraded their IAT membership being elected as a full voting member and can now use the designatory letters MIAT Michael Brown Lois Byrom Chelsea Cavanagh Casey Collins Bruna Delfini Nicola Evans Bailey Daniel Gates Leane Holyoak Adam Johnson Joanne King Andrea Kirton Asha Konde Heather Large Lauren Mackinnon Sian Murphy Michael Penn Pauline Reading Izabela Sabok April Shipton Rebecca Towns Ian Wilson Carl Wright Congratulations to all on their achievement Anyone who has obtained their Level 3 IAT qualification along with a Practical Animal Handling Health Module can apply for MIAT status Further details on requirements and how to apply can be found on the IAT website or from the IAT Administrator admin iat org uk 11

THE FUTURE IS TODAY FIRST CALL for PAPER POSTER or WORKSHOP PRESENTATIONS Delegate registration opens ONLINE Monday 10th September Venue North West England ALL THREE ANNOUNCEMENTS APPEAR IN THIS ISSUE Please look at the information on them We offer generous incentives for participation in the Scientific Programme at Congress So why not contact us with YOUR IDEAS AND SUGGESTIONS TO PRESENT A PAPER POSTER or WORKSHOP Workshop submissions of varying lengths ranging from 1 3 hours will be catered for over three days and many will be repeated ALL SUBMISSION FORMS ARE ON THE WEBSITE DO YOU HAVE COLLEAGUES WHO YOU THINK MIGHT BE INTERESTED IN PRESENTING BUT DO NOT SEE THE BULLETIN REGULARLY THEN WHY NOT ALSO DOWNLOAD THE 3 ANNOUNCEMENTS FROM THE WEBSITE AND PIN THEM ON YOUR TEA ROOM NOTICE BOARD The Invitation to Participate booklet containing prices a provisional schedule and timings will be distributed in next month s Bulletin To discuss any aspect of Congress or if you have any questions for the Congress Committee please contact us at congress iat org uk

Bulletin July 2018 17 IN MEMORIAM MICHAEL A COIRO SR 1934 2018 IN MEMORIAM MICHAEL A COIRO SR IN MEMORIAM 1934 2018 MICHAEL A COIRO SR 1934 2018 It is with profound sadness that we announce the passing of our founder our leader our father and our friend Michael A Coiro Sr More than 50 years ago Michael started Allentown from literally nothing creating a company dedicated to the values he practiced in his everyday life hard work loyalty service and humility From his example came not just a company whose products have contributed to innumerable advances in medicine but a powerful force for what It is and withwhat profound sadness we announce the passing of our founder our leader our father is good is right in ourthat industry and our friend Michael A Coiro Sr More than 50 years ago Michael started Allentown from nothing creating company dedicated the values practiced his everyday life Itliterally is with profound sadnessathat we announce theto passing of ourhe founder ourin leader our father In his own special way service Michael brightened bettered the ofnot allstarted who him teaching hard loyalty and humility From hisyears example came just knew a Allentown company whose and ourwork friend Michael A Coiro Sr More and than 50 ago lives Michael from by literally example that is a personal journey whose beginning you and whose products havelife contributed to innumerable advances medicine butchange a powerful force for ending what nothing creating a company dedicated to thein values hecan t practiced in his everyday life youhard can t know so you had better enjoy yourself along the way is good andloyalty what isservice right in our work and industry humility From his example came not just a company whose products have contributed to innumerable advances in medicine but a powerful force for what isIn good and what is way right in our of industry his special brightened bettered of most all who knew him teaching Toown paraphrase the Michael lyrics his favoriteand artist fromthe onelives of his famous songs by example that life is a personal journey whose beginning you can t change and whose ending know so youMichael had better enjoy yourself along the Inyou his can t own special way and bettered theway lives of all who knew him teaching He lived a life thatbrightened was full He travelled each and every highway by example that life is a personal journey whose beginning you can t change and whose ending But more much more than this He did it his way you can tTo know so you had betterofenjoy yourselfartist alongfrom the way paraphrase the lyrics his favorite one of his most famous songs Mass of Christian Burialthe willlyrics be celebrated 11am on from Friday June 1st at St John ssongs Church 1282 To paraphrase of his favorite one of his most famous He lived a life that was full Heartist travelled each and every highway Yardville Allentown Rd Allentown Interment will follow in the Parish Cemetery A viewing will But more much more than this He did it his way be held Thursday May 31st from 5 9pm at the Peppler Funeral Home 114 S Main St He lived a life that was full He travelled each and every highway Allentown Memorial contributions may be made in Michael s name to the American Cancer Butwill more much more11am than on this He did it his way Mass be Roman celebrated Friday June 1st at St John s Church 1282 Society oroftoChristian St JohnBurial the Baptist Catholic Church Yardville Allentown Rd Allentown Interment will follow in the Parish Cemetery A viewing will be held Thursday Maywill31st from 5 9pm at on theFriday Peppler Funeral Home 114 S Main St Mass of Christian Burial be celebrated 11am June 1st at St John s Church 1282 Allentown Memorial may be made in Michael s nameCemetery to the American Cancer Yardville Allentown Rd contributions Allentown Interment will follow in the Parish A viewing will Society or to St John the31st Baptist Roman Catholic be held Thursday May from 5 9pm at theChurch Peppler Funeral Home 114 S Main St Allentown Memorial contributions may be made in Michael s name to the American Cancer Society or to St John the Baptist Roman Catholic Church

18 July 2018 Bulletin Zebrafish Husbandry Day in Snowy Sheffield Karen Dunford The Bateson Centre Zebrafish Facility in Sheffield hosted the Zebrafish Husbandry Day on February 28th 2018 The staff in Sheffield did a fantastic job putting together an informative day catering to a wide array of interests within the field The Siberian blast of snow that covered much of the UK also made an appearance adding to the atmosphere of the gathering

Bulletin July 2018 19 There were a total of five presentations all covering different topics Michael Thomas from Sheffield discussed the diets used at their facility Nicola Goodwin from the Laboratory of Molecular Biology Cambridge discussed the standardisation of welfare terms Carole Wilson from the UCL Zebrafish Facility presented ideas on how we should approach environmental enrichment the veterinarian JP Mocho presented information on the different possible anaesthetics and analgesics available for fish and Claire Allen also from Sheffield and organiser of the day examined how the 3Rs have been implemented in protected work Photo Greg Paull The first talk presented covered the specific protocols at Sheffield particularly the larval rearing diets and their reasons for choosing them Previously the larval rearing protocol included keeping the animals in static water until 17 days post fertilisation dpf and the dry feed was moistened prior to use they found that this resulted in undesirable survival rates and looked to improve their protocols An alternative was found and trialled on wild types in which the time spent in static water was reduced dry food was fed to the larvae dry and artemia cysts were extracted using a magnetic cyst remover They found that the new protocol lowered mortality rates for larvae thus they adopted these changes into their husbandry practices albeit they have since been refined further

20 July 2018 Bulletin Nicola Goodwin spoke on her work standardising welfare terms used to describe zebrafish stages of health Whilst constantly being added to the ZFIN database online has all of the terms that have currently been defined https wiki zfin org display ZHWG Zebrafish Health and Welfare Glossary H ome The advantages of using these standardised terms are improved record keeping help move towards the use of standardised GA passports and expansion on our knowledge of phenotypes Although the project already has an extensive collection of terms Nicola projected that in future this would include videos information on causes and possible prevention advice During the lunch break people caught up with old colleagues chatted with new ones discussed what had just been presented but many watched their phones and the snow out of the window worrying about travels home Once back the presentations moved into how the zebrafish community is defining environmental enrichment with Carole Wilson Carole pointed out the laboratory zebrafish environments are a far cry from the natural environment in which they are found even more so than their mammalian counterparts Carole spoke on the difference between the more common structural enrichment available such as gravel and plants Exeter fish team having fun in the snow and non structural enrichment like social interaction and promoting Photo Greg Paull natural behaviours through live feed as well as the potential negative effects of both such as increased aggression and potential biosecurity risks The presentation furthered these ideas towards what should be considered and how the zebrafish community may wish to move forward with the concept Interestingly a talking point that Carole ended with was that the zebrafish community may wish to consider a novel approach called agency which would allow zebrafish to make choices The next presentation by JP Mocho was quite the hot topic anaesthesia and analgesia for fish JP discussed reasons for their use and then covered the main anaesthetics available tricaine MS 222 lidocaine benzocaine and

Bulletin July 2018 21 metomidate 2 Phenoxyethanol and clove oil JP pointed out both the pros and cons of each ones I found particularly interesting was that repeated use of Tricaine may lead to mortality and that metomidate may be the least aversive agent but it may inhibit breeding hormones Photo Claire Allen Last was about the 3Rs and protected work from Claire Allen Claire gave very interesting examples of how refinements have been made or investigated in procedural work Some of these examples were complex and would require training such as larval genotyping instead of adult genotyping a refinement that keeps popping up in zebrafish discussions Another example given and related to JP s presentation was the change to benzocaine for prolonged periods of anaesthetic intubation benzocaine does not block Ca ion channels in the body A rather interesting one was the investigation of leaching of white marbles that were used in the facility they found that leaching was present but negligible However it left me wondering if the plastics commonly used in facilities leach at all as some plastics are more likely to do so than glass After the very interesting presentations and more frantic train cancellation checks whilst either admiring or hating the snow falling outside Claire and Michael gave the attendees a tour of their facility This was a great chance to see another facility and how they function I found this particularly interesting as their system is completely different to ours and I had ample opportunity to ask questions and learn about their system The Zebrafish Husbandry Day was both interesting and varied the different topics led to multiple discussions that continued in the pub and into the organised dinner I would like to thank the Bateson Centre for organising such a day

24 July 2018 Bulletin World Aquaculture Society Conference Las Vegas I n 2017 I along with my colleagues published a paper in the Zebrafish Journal validating the use of swabbing non anaesthetised fish titled A Low Cost Method of Skin Swabbing for the Collection of DNA Samples from Small Laboratory Fish At a previous conference I was approached by the then president of the Zebrafish Husbandry Association who asked if I could attend the workshop held by the Zebrafish Husbandry Association and give an oral presentation on the paper This was a great chance to promote the alternative method of genotyping that has now been used at University of Leicester since around 2012 with great success with both zebrafish and sticklebacks Further work is currently ongoing with a successful award from the NC3Rs to the Norton Laboratory Leicester to assess the stress levels of skin swabbing vs fin clipping and also whether we could improve post operative care for both as currently there is little literature on this topic and what is available mainly focusses on the use of lidocaine On the day of the presentation I was feeling a little nervous as there were members of some of the biggest and most respected zebrafish laboratories in the USA present and as with most new techniques the biggest challenge is convincing users to try it as most of the time people do not like change Just before I was due to present my friend from the USA who is also a facility manager and research assistant approached me and said we tried the swabbing at this point I was like anddddddd We loved it was her response They found comparable levels of DNA with fin clips and they also managed to run PCRs on tricky lines All nerves disappeared and confidence was regained I started the presentation on how we came across this method and moved on to its use to identify male sticklebacks before nuptial developments appeared which results in a reduction of breeding and numbers of fish exposed to the parasite infection then I moved on to if it is possible to use this method on non anaesthetised zebrafish At the end of the presentation there were several questions Some I could not answer due to not having attempted the specific procedures within our facility which is small and resources are limited however it turned out there were a few people other than Carrie who had trialled this method and were very supportive of it and able to answer these questions There was also a great discussion about this and at the end of it everyone from research technicians to principal

Bulletin July 2018 25 investigators were excited to try this method I have recently been in touch to gather more information as we have refined the extraction protocol which enables it to be much faster I stated in the presentation that with good communication between the laboratory and or core facility if the samples are collected in the morning they can be identified by mid afternoon and fish returned the same day This helps laboratories and facilities as fish are not taking up space running costs are less and single housed fish is kept to a minimum time Having worked on this for some time to make it perfect it was time to move on to my next presentation on environmental enrichment for zebrafish I was invited by the NC3Rs to present this at their workshop at Congress Easter couldn t come quick enough Many thanks to the AS ET committee for the bursary and continued support Reporter Carl Breacker DATES FOR YOUR DIARY AS ET Annual Lecture Professor Claudio Stern Two for the price of one twinning the ultimate regeneration 17th October 2018 at 5 00pm Venue London Free admittance interested friends and family welcome To be followed by a wine reception Watch this space for further announcements

Bulletin July 2018 AS ET AS ET Annual Annual Lecture Lecture London London 27 Two for the price of one Two for the price of one one Two for the price of the ultimate twinning twinning the ultimate twinning the ultimate regeneration UHJHQHUDWLRQ UHJHQHUDWLRQ Professor Professor Claudio Claudio Stern Stern BSc BSc PhD PhD DSc DSc FRSB FRSB FRMedSci FRMedSci FRS FRS J JZ Z Young Young Professor Professor of of Anatomy Anatomy University College London University College London ____________________________________________________________ ____________________________________________________________ 4 30pm Wednesday Wednesday 18th October October 2018 5 00pm 2018 4 30pm Wednesday 17th 18th October 2018 Central London London Central Central London Thelecture lecture will be be followed by wine The by a winereception reception The lecturewill will be followed followed by aa wine reception ________________ ________________ Admission Free Admission Free Order Tickets from Order Tickets from http www animalsinscienceeducationtrust org uk annuallecture http www animalsinscienceeducationtrust org uk annuallecture Sponsored by Sponsored by

28 July 2018 Bulletin Patron Professor Lord Naren Patel KT Chair of Trustees Professor Sir Richard Gardner FIAT Hon FRS Secretary to the Trust Ken Applebee OBE FIAT FRSB Trustees Stephen Barnett MSc Hon FRSB Jasmine Barley MSc FIAT Karen J Gardner When Irish eyes are smiling Registered Charity Number 113319 Registered Office 5 South Parade Summertown Oxford OX2 7JL There is a song called When Irish Eyes are Smiling which continues When Irish hearts are happy all the world seems bright and gay and that certainly seemed the case at the IAT Irish Branch Symposium and Trade Day AS ET was delighted to be invited to attend the event again and we shared a stand with the IAT so both organisations were doubly represented Sylvia Mehigan one of the organisers of the day informed us that this was the 7th symposium the branch had arranged and as before it was extremely well attended giving AS ET the opportunity to meet with friends of long standing and make some new ones As well as the trip across the water AS ET also made the trip to Manchester in April for the IAT North West Branch s Symposium and the Steve Moore Poster Competition As always we were made very welcome and it was good to have the opportunity to meet with our supporters in Manchester again The selection of posters was impressive and speakers at the symposium were very thought provoking There has also been a fund raising meeting in recent weeks and there are several events in the pipeline The Special Lecture in October see dates for your diary is free this year although we hope that people will be able to donate to help fund our work There is also The Animals in Science Education Trust 4th bursary competition to attend Congress 2019 Please encourage your more junior staff to enter as it s a wonderful opportunity for someone to attend Congress who may not normally be eligible to attend If you have any doubts about the value of our bursaries this Bulletin issue includes a report from Carl Breacker on his trip to attend a conference to promulgate a refinement for genotyping zebrafish Thanks to all our supporters both corporate and individual who make these bursaries possible

AS ET is a charity set up to advance education and promote excelle Bulletin May 2014 15 the care Bulletin and welfare of animals used in science July 2018 29 To see how you can apply for a bursary visit our website www as et ET isisaacharity up advance education and promote excellence ET charityset set uptoto advance education and promote excellence The companies listed below sponsor AS ET If you would like to join care and welfare of animals used in science e find careout andhow welfare of animals used in science to support us by looking at our website ee how you can for visit website www as et org see how canapply apply for abursary bursary visitour our website www as et org AS ET charity set up up toaadvance advance education excellence in AS ET isis you aa charity set to education and promote AS ET is charity set up to advance education and promote excellence in the care and of animals usededucation ineducation science AS ET ischarity awelfare charity set up to advance advance education and promote excellence in the care and animals used in science AS ET aawelfare charity set up and promote excellence AS ET is ais setof up to to advance and promote excellence in in the care and welfare of animals used in science listed below AS ET If would like totojoin them the care and welfare ofsponsor animals used in science science the care and welfare animals used the care welfare of of animals used in in science ecompanies companies listed sponsor AS ET Ifyou you would join AS ET is aand charity to below advance education and promote excellence inlike the care andin the AS ET is a charity set up to advance education and promote excellence To see how you canused apply for bursary visit how to support usinof by looking atvisit our website To see how you can apply for aa bursary our website www as et org uk welfare of animals science dout out how to support by looking our the care and welfare animals usedat in science To see how you can us apply for a bursary visit website our website www as et org uk To see how you can apply for bursary visit our website www as et org uk how apply aa bursary visit website www as et org uk To To seesee how youyou cancan apply forfor a bursary visit ourour website www as et org uk The companies listed below sponsor AS ET Ifour you would like them Sponsors of AS ET are listed below and to find out more please www as et org uk visit to thejoin website The companies listed below sponsor AS ET If you would like to join them To see how you can apply for asponsor bursary visit website The companies listed below AS ET If you would like to join them The companies listed below sponsor AS ET If you would like to join them The companies listed below sponsor AS ET If you would like to join them www as et org uk The companies listed below sponsor AS ET If you would like to join them find out out how how to to support support us us by by looking looking at at our our website website find find out how to support us by looking at our website find out how to support us by looking at our website find how support looking website find outout how to to support us us by by looking at at ourour The companies listed below sponsor AS ET Ifwebsite you would like to join them find out how to support us by looking at our website

Bulletin July 2018 31 We would like you to review Karen Dunford Readers of the Bulletin will have read the reviews of published papers that appear in some of the editions We would like to have more in the Bulletin but we need you to write some We would like people from any job description in an animal lab whether a technician research assistant NACWO or manager all are welcome to submit a paper review for publication in the Bulletin But for some writing a paper review may seem daunting so here are some tips for those looking for some guidance 1 Picking the right paper There are no set criteria for what sort of paper should be reviewed for the Bulletin but it should relate to animal welfare It may be easier to choose a paper about either a species you are familiar with or a topic that you are familiar with such as a particular procedure or research field This will help to be critical later on in your review But you can always choose something that you are not familiar with either you might learn something To pick out the central ideas use a highlighter during a first read By Guido random Alvarez CC BY 2 0 https creativecommons org licenses by 2 0 via Wikimedia Commons 2 Choose how many authors will be reviewers A review can be done solo or with other colleagues Having multiple reviewers may be useful especially if it is your first time Two heads can be better than one If you choose to have multiple authors have a clear outline of who will cover what portion of the paper to review or decide how to organise your review will the reviews be combined or kept separate If combining two reviews make sure that the final copy is well edited to make it flow

32 July 2018 Bulletin 4 Writing the introduction It may be useful to have a quick introduction talking about the topic this can include the reference to the paper that will be reviewed Tell the audience why either the topic in general matters or why the specific paper matters The paper itself may have this sort of information in its own introduction but you as a reviewer may wish to add your own level of expertise here For example in a paper review about cow lameness I pointed out that animal research does not always take place in a lab and does not always benefit lab animals the cows in question were livestock and the paper mattered in my eyes as it was an example of animal welfare applications in a non scientific setting 3 Writing the summary Any paper review should include a summary of the article remember your audience may not have read it But be concise You will need to briefly summarise the following G G G G the aim or hypothesis the methods used the most important results how the results were interpreted These four points need to be short and should not be taken verbatim from the paper It can be difficult to sift through all of the information presented and distil them down a good rule of thumb is that each above point should be paraphrased into one or two sentences so think carefully about what is the most important piece in that section of the paper A good tip here is to highlight the central points of the paper and then use those in your summary The methods for example can include what was measured without details of equipment or omit specifics of numbers and quantification Remember a review does not need to be as detailed as the original paper the audience can seek out the source material and gather these details themselves The results section can be quite lengthy in the original paper but as a reviewer choose the ones that are the most important to share with your audience particularly those that you wish to discuss in your writing Avoid adding in all of the numerical results and focus instead on the overall statement of the results For example use phrases such as not all of the animals performed the task instead of the exact quantification used by the original authors Follow the same rule for the interpretation of the results and stick to the ones you wish to discuss Overall these points should be no more than a paragraph or two 4 Your review The next part should be your response to the paper and should be based on

Bulletin July 2018 33 the information already included in your summary This portion can be informed by your area of expertise and experience as well as any questions that were raised while you were reading the paper Quick points to look for should include G G G missing information in the paper unclear aspects of the experiment experimental design flaws A review is typically critical Perhaps there was missing information in the paper such as sex or age of the animals which can have an impact on the experiment Or perhaps as an experienced animal technician you noticed an unusual aspect of the husbandry techniques or parameters of the experimental design that could have been impacted by husbandry that were not noted by the original authors Ask if there was anything missing or if there was anything you disagree with or if something wasn t particularly clear in the paper Additionally ask if you agree with the interpretations and conclusions made if you don t make a suggestion on how to repeat the experiment to avoid ambiguity For example an environmental enrichment experiment used food and interpreted their results positively can we interpret food in enrichment studies since it is a requirement for survival An alternative could be a toy But a review does not have to be entirely negative be positive as well As a reviewer be sure to point out aspects of the paper that were interesting especially if you found them enlightening or an exciting new approach to animal welfare As you are reading the paper ask if you are learning anything new or anything applicable to your own work Additionally if possible explain how this paper is ethical sound such as how it fits in with the 3Rs this is not always made explicit by the authors and may take some interpretation 5 Conclusion Conclude your review succinctly restating why the paper is important and what the take away points are This does not have to be copied exactly from what was already said above but can be stated briefly in a sentence or two Hopefully this guide will help and inspire some budding writers to review a paper for the Bulletin We want to hear from you Please contact the editor bulletineditor iat org uk or assistant editor bulletinassistant iat org uk if you wish to submit your review We look forward to reading them

34 July 2018 Bulletin The Photo Competition 2018 This year we had 36 great entries to the photo competition and on the front cover we have the winning picture voted for the Congress delegates a beautiful image of a kingfisher by Ken Lovelock Well done Ken that s a fabulous photo and I m jealous I didn t take it myself Worthy second and third places went to Michala Nightingale and Rachel Dearing respectively Michela s raccoon shot is wonderful I love the posture and the human expression that has been captured in this image Rachel s toad is equally enchanting and I think that s a fabulous piece of photographic composition

Bulletin July 2018 Elsewhere we had a wide range of different animal subject matter From the exotic to the more common place from cats to birds monkeys to insects The exotic Angela Kerton s chameleon and Michala Nightingale s kookaburra The more common place Cats George Stainforth s leopard I m not sure you d want to see that in your back garden Sarah Read s black and white cat perhaps a more acceptable back garden occupant 35

36 July 2018 Bulletin Birds Linda Horan s geese in the park Michala s crow and mallard duck Monkeys Carla Foley s squirrel monkey and Norman Mortell s macaque I think that s the breed

Bulletin July 2018 And insects Ken s butterfly Michala s dragonfly Rachel s bee I think you will all agree with me that these images are all fantastic and the photographers should all be congratulated If you think you would like to see your photos displayed in the Bulletin and they are of suitable subjects please send them along and I will create a gallery feature I look forward to seeing them Carole Wilson 37

Cartoon Competition Winner by Lucy Whitfield Bulletin July 2018 39

40 July 2018 Bulletin Large Animal Research Network LARN meeting Tuesday 15th May 2018 Established four years ago the Large Animal Research network LARN provides support and information to those who use large animals in biomedical veterinary and or agricultural research In 2017 LARN was incorporated into LASA and it is hoped that with their support this network will grow in strength and breadth This year s annual meeting was hosted by University of Bristol who provided a fantastic overview of Bristol s facilities including their 3 Tesla MRI imaging in large animal research With the support of sponsors and LASA the meeting included over 40 researchers animal technicians and facility managers from various UK establishments Bristol s Translational Biomedical Research Centre TBRC is a translational research and educational facility unique in the UK and EU for the benefit of humans animals and the environment The facility includes a research hospital with induction room surgical facilities state of the art imaging and postoperative care for large animals Darren Roper of Langford veterinary services gave an excellent presentation on MRI imaging at TBRC Following on from this Kristina Baker the specialist TBRC technician and facility coordinator gave a fascinating presentation outlining her role within the facility and the responsibilities around arranging and supporting surgical and MRI studies During the refreshment break TBRC arranged a well coordinated schedule of tours around their large animal facilities In between the tours attendees were able to enjoy coffee and pastries whilst browsing the sponsors trade stands Following the break Dr Andrew Broadbent from The Pirbright Institute gave a presentation on his work on a chicken primary B cell culture model to study the pathogenesis and improve the control of immunosuppressive viruses of poultry Whilst poultry may not be initially considered to be large animals it s been agreed that they should be included within LARN as this is a better fit than the groups supporting more traditional laboratory animal species Dr Atticus Hainsworth a Neuroscientist and expert in stroke dementia and Vascular Cognitive Impairment at St George s University of London gave a really interesting presentation on using large animals to model human brain diseases

Bulletin July 2018 41 During the lunchbreak TBRC arranged for groups of attendees to tour their surgical and MRI facilities which received excellent feedback The tour groups were then welcomed back with a delicious lunch and a further opportunity to network with colleagues and trade sponsors Dr Sarah Watts from DSTL Porton Down then gave a really insightful presentation on trauma research projects This is an area of research which so many people don t fully appreciate in terms of what has been achieved to date and which so many have false ideas of what it entails Dr Thomas Krieg from the Department of Medicine at the University of Cambridge gave a detailed presentation on the translation of a novel drug through the use of large animals Following on from this Dr Belinda Farnfield the NVS at DSTL Porton Down gave a presentation on some of the issues and concerns around using pigs and other large animal species in biomedical research projects From the ethical considerations outlined it was really interesting that so many attendees had clearly been presented with the same dilemmas to address and that there was some general consensus on the approaches taken Dr Jordi Lopez Tremoleda from the Centre for Trauma Sciences at the Queen Mary University of London gave an engaging talk about the impact of imaging on animal welfare and scientific validity Professor Michael Lee a ruminant nutritionist at Rothamsted Research University of Bristol gave an absorbing presentation on sustainable ruminant livestock research From the number of questions at the end of his presentation it s fair to say that this was of riveting interest to so many in the room

42 July 2018 Bulletin Given the level of interest in all of the tours and talks time was tight by the end of the meeting such that the final scheduled presentation on the FELASA Working Group for Farm Animals was therefore only briefly outlined This Working Group which includes members from the UK Germany Switzerland Norway France Greece and Italy aims to formulate farm animal health screening housing and procurement considerations including reviewing current FELASA documents e g FELASA recommendations for the health monitoring of experimental units of calves sheep goats and pigs The aim is to present the outcome of the Working Group at the FELASA 14th Congress in June 2019 If you d like to find out more please email the UK contact Dr Ryan Waters Ryan Waters pirbright ac uk The LARN section convenors Rob Bond and Lauren Cresser would like to extend sincere thanks to TBRC for preparing and hosting the meeting the diverse group of speakers who did a fantastic job of sharing their expertise the sponsors who enabled the meeting to take place and to LASA who provided so much support in the lead up to the meeting and on the day Thank you all Networking events like these are important opportunities to share good practice and new ideas with those working in similar organisations LARN is relevant to scientists technicians a wide range of support staff and interested groups who are either actively involved in research using large animals or who may in the future have a requirement to understand more about large animal models It is by assembling a diverse range of interests backgrounds and skills relating to large animals that LARN is able to progress and further develop in the future With this in mind the 2018 LASA annual meeting will include presentations and workshops on large animals Please contact LASA info lasa co uk if you wish to be included in email correspondence about future meetings and progress Reporters Lauren Cresser and Robert Bond

Bulletin July 2018 Branch Innovation and Networking Group BING Tuesday 15th May 2018 Another day for the IAT Youth Reps It was a lovely warm day spent in London catching up previous minutes from the last meeting in September 2017 We welcomed everyone including a new representative Unfortunately some were unable to attend today There was a discussion about the previous meeting regarding congress i e competitions ice breakers and talks For those that are not aware there is an IAT Facebook page that you can join and get involved on some of the action such as Waggy Wednesdays and Throwback Thursdays to have some fun and get in touch with other members of the community from other branches Once the meeting was concluded we all went to a nearby pub to unwind and enjoy ourselves before heading home For anyone interested to become a member and to come along to these meetings then please get in touch with your branch representative We are always looking for new innovative members to join that can bring new ideas and insights to the team Reporter Melissa Vaughan 43

44 July 2018 Bulletin

Bulletin July 2018 45 NIO Forum N amed Information Officers are required to find and provide information for those who request it As the role is a relatively new addition to British laboratory animal facilities some NIOs may find it challenging to uncover requested information expeditiously Below are some resources that NIOs may find useful in their investigations The 3Rs and Knowledge Sharing A JRC Science for Policy Report has been published now available as a pdf and was compiled in response to a public initiative to stop the use of animals in science This report entitled Accelerating progress in the Replacement Reduction and Refinement of animal testing through better knowledge sharing is the result of a large scale survey of how scientists find and share new information within the industry referred to as knowledge sharing specifically 3Rs related and suggests how this can be improved through various means The authors state that they will be making a publically available inventory of knowledge sharing sources that will ideally be used as a research tool in the future so watch for further updates This may be of interest to NIOs but also to scientists and trainers who are looking to promote their work and to see what methods old and new are most effective The report can be found online at https ec europa eu jrc en publication eur scientific and technicalresearch reports accelerating progress replacement reduction andrefinement animal testing through better

Bulletin July 2018 DIARY Dates 16 and 17 July NACWO workshop Central London Details info learningcurvedevelopment co uk 30 October RSPCA UFAW Rodent and Rabbit Welfare Meeting Central London Details research animals rspca org uk 10 September Congress 2019 Online registration opens See page 13 2 November The Andrew Blake Tribute Award closing date Details on page 15 15 September IAT Inter branch Fishing Match Wellingborough Details on page 44 16 November Congress 2019 Closing date for Paper and Workshop submissions See pages 12 and 14 21 September RSPCA Lay Members Forum Edinburgh Details research animals rspca org uk 17 October AS ET Annual Lecture London Details on page 27 28 October 1st November AALAS National Meeting Details www aalas org nationalmeeting Front cover Kingfisher by Ken Lovelock 27 29 November LASA Conference Birmingham Details http www lasa co uk meetings in fo lasa co uk 47