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A Chemo-Sensitivity Assay   Personalized Cancer Therapy and Drug Discovery
Background     Current State of Technology    HTCA  Human Tumor Clonogenic Assay  - 1st assay used to predict chemo effect...
Background   Market    Precision Therapeutics, Pittsburgh PA, www.precisiontherapeutics.com    ChemoFx   - plate based ass...
Scenarios For Chemo-Sensitivity Assay Biopsy  MD  Pharma   Academia  Sample Clinical Laboratory Black Box     Hardware Sam...
Optimized Cell Based Sample Prep  1 - Cell Plating   e.g. Biopsy  clinical  immortalized  drug discovery   Day 0   2     T...
Distillation of Data LEVEL 0 LEVEL 1 LEVEL 2 LEVEL 3 LEVEL 4 LEVEL 5  Raw Data Collection Individual Cell Profiling Cellul...
Automated Data Process  cont     LEVEL ZERO  LEVEL ONE  Raw Data  Individual Cell Profiling      p-ATM signal SA c,t,d,dd ...
Distillation Process Level Two Cellular Data Integration  Single well   at every time point, drug and drug dose  Threshold...
Distillation Process Level Three  Extraction of Time Dependence  Cell Numbers   H2AX     Sad,dd  t  NH d,dd t  Nd,dd t   T...
Distillation Process Level Four  Extraction of Dose   Drug Dependence  Cell Number  dd2  dd3  Cell Number  dd1  ddoptimal ...
Distillate Level Five     Personalized Dosing        ddoptimal x correction Factor  e.g. BMI   A  p-ATM, H    H2AX, S  Sig...
Distillation of Data LEVEL 0 LEVEL 1 LEVEL 2 LEVEL 3 LEVEL 4 LEVEL 5  Raw Data Collection Individual Cell Profiling Cellul...
Level 3 Distillation of Taxol Treated MCF7 Cells gH2AX positive cells  fraction positive cells  1.2     Maximum appears to...
Level 3 Distillation of Taxol Treated MCF7 Cells     5uM signal reaches low at  4h, no change    All others similar     Co...
Level 4 Distillation of Taxol Treated MCF7 Cells Nd1 t,dd      All dd kill     Titration dependent Kill level  toptimal   ...
Level 3 Distillation of Etoposide Treated HeLa Cells    Good separation  SA     No Decrease in  SA   Etoposide  0.08uM Eto...
Summary MCF7   HeLa    MCF7    No information prior to Taxol treatment    Dose, treatment, timing unknown    loss viabilit...
Drug Discovery   Clinical Application    Rapid assessment of unknown drug efficacy    Within 2 days  48h     Drug Discover...